LOCUS       sequence-352024-        6044 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transposon allowing the expression of the fluorescent protein 
            mEos3.2 in multiple mycoplasma species, under the control of the 
            P438 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6044)
  AUTHORS   Rideau F, Villa A, Belzanne P, Verdier E, Hosy E, Arfi Y
  TITLE     Imaging Minimal Bacteria at the Nanoscale: a Reliable and Versatile 
            Process to Perform Single-Molecule Localization Microscopy in 
            Mycoplasmas.
  JOURNAL   Microbiol Spectr. 2022 Jun 29;10(3):e0064522. doi: 
            10.1128/spectrum.00645-22. Epub 2022 May 31.
  PUBMED    35638916
REFERENCE   2  (bases 1 to 6044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol 
            Spectr."; date: "2022-06-29"; volume: "10(3):e0064522. doi"; pages: 
            " 10.1128/spectrum.00645-22. Epub 2022 May 31"
COMMENT     Sequence Label: pMT85_P438-mEOS3.2_GentaR
FEATURES             Location/Qualifiers
     source          1..6044
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1098..1114
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1103..1125
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1122..1138
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1122..1138
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1217..1239)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3666..4254
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4155..4174
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4408..4425
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gataaagtcc gtataattgt gtaaaaaccc atagctttgg acacacacta gtttagtatt
       61 tagaattaat aaagtatgtc agctattaaa ccagatatga aaattaaatt aagaatggaa
      121 ggaaatgtaa atggacatca ttttgtaata gatggagatg gtactggtaa accatttgaa
      181 ggtaaacaat caatggattt agaagttaaa gaaggtggtc cattaccatt tgcttttgat
      241 attttaacta ctgcttttca ttatggtaat agagtttttg ctaaatatcc agataatatt
      301 caagattatt ttaaacaatc atttccaaaa ggttattcat gagaaagatc attaactttt
      361 gaagatggtg gtatttgtaa tgctagaaat gatattacta tggaaggtga tactttttat
      421 aataaagtta gattttatgg tactaatttt ccagctaatg gtccagttat gcaaaaaaaa
      481 actttaaaat gagaaccatc aactgaaaaa atgtatgtta gagatggtgt tttaactggt
      541 gatattgaaa tggctttatt attagaaggt aatgctcatt atagatgtga ttttagaact
      601 acttataaag ctaaagaaaa aggtgttaaa ttaccaggtg ctcattttgt tgatcattgt
      661 attgaaattt tatcacatga taaagattat aataaagtta aattatatga acatgctgtt
      721 gctcattcag gtttaccaga taatgctaga agatagctta agaaattaaa gttggttcat
      781 tcaaagttac aattcgttta aaaagtgaat aattaattat taattaattt attaaaaaac
      841 aaccaaaagg ttgtttttta ttttttaaat ctagtggatc tcataaaaat gtatcctaaa
      901 tcaaatatcg gacaagcagt gtctgatata acaaaaaatc gatttaatag acacattaac
      961 agcactgttt ttatgtgtgc gataatttat aatatttcgg acggttgcgg atccacccgc
     1021 aattactgtg agttagctca ctcattaggc accccaggct ttacacttta tacttccggc
     1081 tcgtatattg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacct
     1141 tgattacgga attcacggcc gggggggcca ccccaccaat tgacgcggcc gcaactctag
     1201 aggattcatc ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     1261 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     1321 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc
     1381 cggcaccaga agcggtgccg gaaagctggc tggagtgcga tcttcctgag gccgatactg
     1441 tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc gcccatctac accaacgtga
     1501 cctatcccat tacggtcaat ccgccgtttg ttcccacgga gaatccgacg ggttgttact
     1561 cgctcacatt taatgttgat gaaagctggc tacaggaagg ccagacgcga attatttttg
     1621 atggctagaa gctttaggat gaatggattt attcttcaag aaaatacatc aattttgata
     1681 agtagaaatg gtaaaaacat tgtatagcat tttacacagg agtctggact tgactgagtt
     1741 tatggaagaa gttttaattg atgataatat ggtttttgat attgataatt taaaaggatt
     1801 tcttaatgat accagttcat ttgggtttat agctaaagaa aataataaaa ttataggatt
     1861 tgcatattgc tatacacttt taagacctga tggaaaaaca atgttttatt tacactcaat
     1921 aggaatgtta cctaactatc aagacaaagg ttatggttca aaattattat cttttattaa
     1981 ggaatattct aaagagattg gttgttctga aatgttttta ataactgata aaggtaatcc
     2041 tagagcttgc catgtatatg aaaaattagg tggtaaaaat gattataaag atgaaatagt
     2101 atatgtatat gattatgaaa aaggtgataa ataaatgaat atagttgaaa atgaaatatg
     2161 tataagaact ttaatagatg atgattttcc tttgatgtta aaatggttaa ctgatgaaag
     2221 agtattagaa ttttatggtg gtagagataa aaaatataca ttagaatcat taaaaaaaca
     2281 ttatacagag ccttgggaag atgaagtttt tagagtaatt attgaatata acaatgttcc
     2341 tattggatat ggacaaatat ataaaatgta tgatgagtta tatactgatt atcattatcc
     2401 aaaaactgat gagatagtct atggtatgga tcaatttata ggagagccaa attattggag
     2461 taaaggaatt ggtacaagat atattaaatt gatttttgaa tttttgaaaa aagaaagaaa
     2521 tgctaatgca gttattttag accctcataa aaataatcca agagcaataa gggcatacca
     2581 aaaatctggt tttagaatta ttgaagattt gccagaacat gaattacacg agggcaaaaa
     2641 agaagattgt tatttaatgg aatatagata tgatgataat gccacaaatg ttaaggcaat
     2701 gaaatattta attgagcatt actttgataa tttcaaagta gatagtattg aaataatcgg
     2761 tagtggttat gatagtgtgg catatttagt taataatgaa tacattttta aaacaaaatt
     2821 tagtactaat aagaaaaaag gttatgcaaa agaaaaagca atatataatt ttttaaatac
     2881 aaatttagaa actaatgtaa aaattcctaa tattgaatat tcgtatatta gtgatgaatt
     2941 atctatacta ggttataaag aaattaaagg aactttttta acaccagaaa tttattctac
     3001 tatgtcagaa gaagaacaaa atttgttaaa acgagatatt gccagttttt taagacaaat
     3061 gcacggttta gattatacag atattagtga atgtactatt gataataaac aaaatgtatt
     3121 agaagagtat atattgttgc gtgaaactat ttataatgat ttaactgata tagaaaaaga
     3181 ttatatagaa agttttatgg aaagactaaa tgcaacaaca gtttttgagg gtaaaaagtg
     3241 tttatgccat aatgatttta gttgtaatca tctattgtta gatggcaata atagattaac
     3301 tggaataatt gattttggag attctggaat tatagatgaa tattgtgatt ttatatactt
     3361 acttgaagat agtgaagaag aaataggaac aaattttgga gaagatatat taagaatgta
     3421 tggaaatata gatattgaga aagcaaaaga atatcaagat atagttgaag aatattatcc
     3481 tattgaaact attgtttatg gaattaaaaa tattaaacag gaatttatcg aaaatggtag
     3541 aaaagaaatt tataaaagga cttataaaga ttaataaaga tctacgaagg catgaccaaa
     3601 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     3661 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     3721 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3781 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     3841 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3901 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3961 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     4021 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     4081 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     4141 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     4201 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     4261 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     4321 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     4381 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     4441 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcac gctagcggat
     4501 ctcataaaaa tgtatcctaa atcaaatatc ggacaagcag tgtctgttat aacaaaaaat
     4561 cgatttaata gacacattaa cagcactgtt tttatgtgtg cgataattta taatatttcg
     4621 gacggttgcg gtaccctttt acacaattat acggacttta tcctgcaggg gcccaattgt
     4681 gtaaaagtaa aaaggccata taacagtcct tttacggtac aatgttttta acgacaaaaa
     4741 catacccagg aggactttta catgacccaa gtacatttta cactgaaaag cgaagagatt
     4801 caaagcatta ttgaatattc tgtaaaggat gacgtttcta aaaatatttt aacaacggta
     4861 tttaatcaac taatggaaaa tcaacgaaca gaatatattc aagcaaaaga atatgaacga
     4921 acagaaaacc gacaaagtca acgaaatggc tattatgagc gcagctttac gacacgtgta
     4981 ggcacgctag aattaaaagt acccagaaca cgtgatggcc atttttcacc cacagtgttt
     5041 gaacgttatc aacgaaacga aaaagccctc atggcttcaa tgttggaaat gtatgtatca
     5101 ggcgtttcaa ctcgtaaagt atcaaaaatt gtggaagaac tttgtggtaa atccgtctct
     5161 aagtccttcg tttctagctt aacagaacag ctagaaccta tggttaacga gtgacagaat
     5221 cgtttattat cagaaaaaaa ttatccttac ttaatgaccg atgtactcta tataaaagta
     5281 cgagaagaaa atcgagtact ctcaaaaagc tgtcatatag cgattggaat aaccaaagat
     5341 ggcgaccgtg aaattatcgg cttcatgatt caaagtggcg aaagcgaaga gacctggaca
     5401 acattttttg aatacctaaa agaacgcggt ttacaaggta cggaactcgt tatttctgat
     5461 gcgcacaaag gattagtctc tgccattaga aaatccttca ccaacgtaag ttggcaaaga
     5521 tgccaagttc acttcctaag aaatatcttt accaccattc ctaaaaaaaa ttcaaaatct
     5581 ttcagagaag ctgttaaagg aatttttaag ttcacagata ttaacttagc gcgtgaggct
     5641 aaaaatcgat tgattcatga ttatatcgat caaccaaaat attcaaaagc ttgcgcatca
     5701 ttggatgatg gattcgaaga cgcctttcaa tataccgtac aaggaaattc ccacaatcga
     5761 ctaaagagta ccaatctaat tgaacgactg aatcaagaag tacgcagaag agaaaagatt
     5821 attcgcatct tccccaatca aacatcagcc aatcgcttaa ttggagccgt tcttatggac
     5881 ctacatgatg aatggattta ttcttcaaga aaatacatca attttgataa gtagaaatgg
     5941 taaaaacatt gtatagcatt ttacacagga gtctggactt gactcacttc ctttattatt
     6001 tttcattttt ttgacctcga gggggggccc accatacagc tgac
//
