LOCUS       sequence-352046-        4249 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transposon allowing the expression of the fluorescent protein 
            mEos3.2 in multiple mycoplasma species, under the control of the 
            synmyco promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Rideau F, Villa A, Belzanne P, Verdier E, Hosy E, Arfi Y
  TITLE     Imaging Minimal Bacteria at the Nanoscale: a Reliable and Versatile 
            Process to Perform Single-Molecule Localization Microscopy in 
            Mycoplasmas.
  JOURNAL   Microbiol Spectr. 2022 Jun 29;10(3):e0064522. doi: 
            10.1128/spectrum.00645-22. Epub 2022 May 31.
  PUBMED    35638916
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol 
            Spectr."; date: "2022-06-29"; volume: "10(3):e0064522. doi"; pages: 
            " 10.1128/spectrum.00645-22. Epub 2022 May 31"
COMMENT     Sequence Label: pMT85_Psynmyco-mEos3.2_GentaR
FEATURES             Location/Qualifiers
     source          1..4249
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1981..2569
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2470..2489
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2723..2740
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aaatggtaaa aacattgtat agcattttac acaggagtct ggacttgact gagtttatgg
       61 aagaagtttt aattgatgat aatatggttt ttgatattga taatttaaaa ggatttctta
      121 atgataccag ttcatttggg tttatagcta aagaaaataa taaaattata ggatttgcat
      181 attgctatac acttttaaga cctgatggaa aaacaatgtt ttatttacac tcaataggaa
      241 tgttacctaa ctatcaagac aaaggttatg gttcaaaatt attatctttt attaaggaat
      301 attctaaaga gattggttgt tctgaaatgt ttttaataac tgataaaggt aatcctagag
      361 cttgccatgt atatgaaaaa ttaggtggta aaaatgatta taaagatgaa atagtatatg
      421 tatatgatta tgaaaaaggt gataaataaa tgaatatagt tgaaaatgaa atatgtataa
      481 gaactttaat agatgatgat tttcctttga tgttaaaatg gttaactgat gaaagagtat
      541 tagaatttta tggtggtaga gataaaaaat atacattaga atcattaaaa aaacattata
      601 cagagccttg ggaagatgaa gtttttagag taattattga atataacaat gttcctattg
      661 gatatggaca aatatataaa atgtatgatg agttatatac tgattatcat tatccaaaaa
      721 ctgatgagat agtctatggt atggatcaat ttataggaga gccaaattat tggagtaaag
      781 gaattggtac aagatatatt aaattgattt ttgaattttt gaaaaaagaa agaaatgcta
      841 atgcagttat tttagaccct cataaaaata atccaagagc aataagggca taccaaaaat
      901 ctggttttag aattattgaa gatttgccag aacatgaatt acacgagggc aaaaaagaag
      961 attgttattt aatggaatat agatatgatg ataatgccac aaatgttaag gcaatgaaat
     1021 atttaattga gcattacttt gataatttca aagtagatag tattgaaata atcggtagtg
     1081 gttatgatag tgtggcatat ttagttaata atgaatacat ttttaaaaca aaatttagta
     1141 ctaataagaa aaaaggttat gcaaaagaaa aagcaatata taatttttta aatacaaatt
     1201 tagaaactaa tgtaaaaatt cctaatattg aatattcgta tattagtgat gaattatcta
     1261 tactaggtta taaagaaatt aaaggaactt ttttaacacc agaaatttat tctactatgt
     1321 cagaagaaga acaaaatttg ttaaaacgag atattgccag ttttttaaga caaatgcacg
     1381 gtttagatta tacagatatt agtgaatgta ctattgataa taaacaaaat gtattagaag
     1441 agtatatatt gttgcgtgaa actatttata atgatttaac tgatatagaa aaagattata
     1501 tagaaagttt tatggaaaga ctaaatgcaa caacagtttt tgagggtaaa aagtgtttat
     1561 gccataatga ttttagttgt aatcatctat tgttagatgg caataataga ttaactggaa
     1621 taattgattt tggagattct ggaattatag atgaatattg tgattttata tacttacttg
     1681 aagatagtga agaagaaata ggaacaaatt ttggagaaga tatattaaga atgtatggaa
     1741 atatagatat tgagaaagca aaagaatatc aagatatagt tgaagaatat tatcctattg
     1801 aaactattgt ttatggaatt aaaaatatta aacaggaatt tatcgaaaat ggtagaaaag
     1861 aaatttataa aaggacttat aaagattaat aaagatctac gaaggcatga ccaaaatccc
     1921 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     1981 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     2041 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     2101 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     2161 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     2221 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     2281 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     2341 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     2401 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     2461 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     2521 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     2581 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     2641 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     2701 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     2761 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcacgctag cggatctcat
     2821 aaaaatgtat cctaaatcaa atatcggaca agcagtgtct gttataacaa aaaatcgatt
     2881 taatagacac attaacagca ctgtttttat gtgtgcgata atttataata tttcggacgg
     2941 ttgcggtacc cttttacaca attatacgga ctttatcctg caggggccca attgtgtaaa
     3001 agtaaaaagg ccatataaca gtccttttac ggtacaatgt ttttaacgac aaaaacatac
     3061 ccaggaggac ttttacatga cccaagtaca ttttacactg aaaagcgaag agattcaaag
     3121 cattattgaa tattctgtaa aggatgacgt ttctaaaaat attttaacaa cggtatttaa
     3181 tcaactaatg gaaaatcaac gaacagaata tattcaagca aaagaatatg aacgaacaga
     3241 aaaccgacaa agtcaacgaa atggctatta tgagcgcagc tttacgacac gtgtaggcac
     3301 gctagaatta aaagtaccca gaacacgtga tggccatttt tcacccacag tgtttgaacg
     3361 ttatcaacga aacgaaaaag ccctcatggc ttcaatgttg gaaatgtatg tatcaggcgt
     3421 ttcaactcgt aaagtatcaa aaattgtgga agaactttgt ggtaaatccg tctctaagtc
     3481 cttcgtttct agcttaacag aacagctaga acctatggtt aacgagtgac agaatcgttt
     3541 attatcagaa aaaaattatc cttacttaat gaccgatgta ctctatataa aagtacgaga
     3601 agaaaatcga gtactctcaa aaagctgtca tatagcgatt ggaataacca aagatggcga
     3661 ccgtgaaatt atcggcttca tgattcaaag tggcgaaagc gaagagacct ggacaacatt
     3721 ttttgaatac ctaaaagaac gcggtttaca aggtacggaa ctcgttattt ctgatgcgca
     3781 caaaggatta gtctctgcca ttagaaaatc cttcaccaac gtaagttggc aaagatgcca
     3841 agttcacttc ctaagaaata tctttaccac cattcctaaa aaaaattcaa aatctttcag
     3901 agaagctgtt aaaggaattt ttaagttcac agatattaac ttagcgcgtg aggctaaaaa
     3961 tcgattgatt catgattata tcgatcaacc aaaatattca aaagcttgcg catcattgga
     4021 tgatggattc gaagacgcct ttcaatatac cgtacaagga aattcccaca atcgactaaa
     4081 gagtaccaat ctaattgaac gactgaatca agaagtacgc agaagagaaa agattattcg
     4141 catcttcccc aatcaaacat cagccaatcg cttaattgga gccgttctta tggacctaca
     4201 tgatgaatgg atttattctt caagaaaata catcaatttt gataagtag
//
