LOCUS       sequence-352050-        2550 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MPRA v3 empty backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Tewhey R, Kotliar D, Park DS, Liu B, Winnicki S, Reilly SK, Andersen
            KG, Mikkelsen TS, Lander ES, Schaffner SF, Sabeti PC
  TITLE     Direct Identification of Hundreds of Expression-Modulating Variants 
            using a Multiplexed Reporter Assay.
  JOURNAL   Cell. 2016 Jun 2;165(6):1519-29. doi: 10.1016/j.cell.2016.04.027.
  PUBMED    27259153
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.04"; journalName: "Cell"; date: "2016-06-2- 2";
            volume: "165"; issue: "6"; pages: "1519-29"
COMMENT     Sequence Label: pMPRAv3:∆luc:∆xbaI
FEATURES             Location/Qualifiers
     source          1..2550
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(142..730)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(222..241)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(930..1721,1722..1790))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    1895..1943
                     /note="synthetic polyadenylation signal"
     misc_feature    1957..2048
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     1997..2016
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    2152..2273
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2163..2182)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2217..2236
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2521..2538)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
       61 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
      121 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
      181 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
      241 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
      301 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
      361 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
      421 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
      481 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
      541 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
      601 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
      661 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
      721 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
      781 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
      841 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
      901 cggccgcaaa tgctaaacca ctgcagtggt taccagtgct tgatcagtga ggcaccgatc
      961 tcagcgatct gcctatttcg ttcgtccata gtggcctgac tccccgtcgt gtagatcact
     1021 acgattcgtg agggcttacc atcaggcccc agcgcagcaa tgatgccgcg agagccgcgt
     1081 tcaccggccc ccgatttgtc agcaatgaac cagccagcag ggagggccga gcgaagaagt
     1141 ggtcctgcta ctttgtccgc ctccatccag tctatgagct gctgtcgtga tgctagagta
     1201 agaagttcgc cagtgagtag tttccgaaga gttgtggcca ttgctactgg catcgtggta
     1261 tcacgctcgt cgttcggtat ggcttcgttc aactctggtt cccagcggtc aagccgggtc
     1321 acatgatcac ccatattatg aagaaatgca gtcagctcct tagggcctcc gatcgttgtc
     1381 agaagtaagt tggccgcggt gttgtcgctc atggtaatgg cagcactaca caattctctt
     1441 accgtcatgc catccgtaag atgcttttcc gtgaccggcg agtactcaac caagtcgttt
     1501 tgtgagtagt gtatacggcg accaagctgc tcttgcccgg cgtctatacg ggacaacacc
     1561 gcgccacata gcagtacttt gaaagtgctc atcatcggga atcgttcttc ggggcggaaa
     1621 gactcaagga tcttgccgct attgagatcc agttcgatat agcccactct tgcacccagt
     1681 tgatcttcag catcttttac tttcaccagc gtttcggggt gtgcaaaaac aggcaagcaa
     1741 aatgccgcaa agaagggaat gagtgcgaca cgaaaatgtt ggatgctcat actcgtcctt
     1801 tttcaatatt attgaagcat ttatcagggt tactagtacg tctctcaagg ataagtaagt
     1861 aatattaagg tacgggaggt attggacagg ccgcaataaa atatctttat tttcattaca
     1921 tctgtgtgtt ggttttttgt gtgaatcgat agtactaaca tacgctctcc atcaaaacaa
     1981 aacgaaacaa aacaaactag caaaataggc tgtccccagt gcaagtgcag gtgccagaac
     2041 atttctctgg cctaactggc cggtacctga gctcgctagc ctcgaggata tcaagatctg
     2101 gcctcggcgg ccaagctagt cggggcggcc ggccgcttcg agcagacatg ataagataca
     2161 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     2221 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     2281 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
     2341 agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc gtcgaccgat gcccttgaga
     2401 gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt
     2461 atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct cttccgcttc
     2521 ctcgctcact gactcgctgc gctcggtcgt
//
