LOCUS       sequence-352057-        9323 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible expression of Human NMI in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9323)
  AUTHORS   Rosa FF, Pires CF, Kurochkin I, Halitzki E, Zahan T, Arh N, 
            Zimmermannova O, Ferreira AG, Li H, Karlsson S, Scheding S, Pereira 
            CF
  TITLE     Single-cell transcriptional profiling informs efficient 
            reprogramming of human somatic cells to cross-presenting dendritic 
            cells.
  JOURNAL   Sci Immunol. 2022 Mar 4;7(69):eabg5539. doi: 
            10.1126/sciimmunol.abg5539. Epub 2022 Mar 4.
  PUBMED    35245086
REFERENCE   2  (bases 1 to 9323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2022-03-4"; volume: "7(69):eabg5539. doi"; pages: 
            " 10.1126/sciimmunol.abg5539. Epub 2022 Mar 4"
COMMENT     Sequence Label: pFUW-TetO-NMI
FEATURES             Location/Qualifiers
     source          1..9323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    join(20..38,39..61,62..80,81..103,104..122,123..145,
                     146..164,165..187,188..206,207..229,230..248,249..271,
                     272..290)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        323..361
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     358..382
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    1421..2009
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1474..1494)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1892..1903)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2012..2028)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2013..2029)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2534..2714
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2740..2757)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2746..2970
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3016..3444
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3103..3122)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3313..3334
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3453..3473)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3458..3787
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3638..3773
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3700..3719
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3835..3882
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3901..4275
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4405..4526
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4442..4461)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4496..4515
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4575..4591)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4575..4591)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4588..4610)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4599..4615
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4623..4629,4630..4647,4648..4653))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4668..4689
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4806..4823)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4977..5565)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5057..5076)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5736..6527,6528..6596))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6359..6378
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6597..6701)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6776..6795)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6967..7346
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7348..7546
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             7564..7744
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7791..7916
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8409..8642
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8827..8871
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9169..9286
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tagatctcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
       61 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
      121 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
      181 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
      241 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
      301 tcgagctcgg tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc
      361 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
      421 aagacaccgg gaccgatcca gcctccgcgg ccccgaattc accggttcta gaatggaagc
      481 tgataaagat gacacacaac aaattcttaa ggagcattcg ccagatgaat ttataaaaga
      541 tgaacaaaat aagggactaa ttgatgaaat tacaaagaaa aatattcagc taaggaagga
      601 gatccaaaag cttgaaacgg agttacaaga ggctaccaaa gaattccaga ttaaagagga
      661 tattcctgaa acaaagatga aattcttatc agttgaaact cctgagaatg acagccagtt
      721 gtcaaatatc tcctgttcgt ttcaagtgag ctcgaaagtt ccttatgaga tacaaaaagg
      781 acaagcactt atcacctttg aaaaagaaga agttgctcaa aatgtggtaa gcatgagtaa
      841 acatcatgta cagataaaag atgtaaatct ggaggttacg gccaagccag ttccattaaa
      901 ttcaggagtc agattccagg tttatgtaga agtttctaaa atgaaaatca atgttactga
      961 aattcctgac acactgcgtg aagatcaaat gagagacaaa ctagagctga gcttttcaaa
     1021 gtcccgaaat ggaggcggag aggtggaccg cgtggactat gacagacagt ccgggagtgc
     1081 agtcatcacg tttgtggaga ttggagtggc tgacaagatt ttgaaaaaga aagaataccc
     1141 tctttatata aatcaaactt gccatagagt tactgtttct ccatacacag aaatacactt
     1201 gaaaaagtat cagatatttt caggaacatc taagaggaca gtgcttctga caggaatgga
     1261 aggcattcaa atggatgaag aaattgtgga ggatttaatt aacattcact ttcaacgggc
     1321 aaagaatgga ggtggagaag tagatgtggt caagtgttct ctaggtcaac ctcacatagc
     1381 atactttgaa gaatagttcg aagatatcaa gcttatcgat aatcaacctc tggattacaa
     1441 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     1501 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     1561 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     1621 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     1681 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     1741 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     1801 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     1861 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     1921 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     1981 tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag acctagaaaa
     2041 acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga
     2101 agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat
     2161 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     2221 gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg
     2281 ctacttccct gattggcaga actacacacc agggccaggg atcagatatc cactgacctt
     2341 tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg
     2401 agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga
     2461 agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca
     2521 tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     2581 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     2641 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     2701 gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     2761 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     2821 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     2881 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     2941 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     3001 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     3061 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     3121 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     3181 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     3241 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     3301 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     3361 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     3421 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     3481 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     3541 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     3601 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     3661 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     3721 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     3781 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac
     3841 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
     3901 atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc
     3961 gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt
     4021 gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac
     4081 aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag
     4141 gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag
     4201 ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc
     4261 gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg
     4321 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     4381 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     4441 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     4501 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     4561 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     4621 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     4681 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     4741 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     4801 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     4861 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     4921 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     4981 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     5041 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     5101 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     5161 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     5221 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     5281 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     5341 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     5401 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     5461 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     5521 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     5581 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     5641 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     5701 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     5761 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     5821 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     5881 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     5941 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     6001 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     6061 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     6121 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     6181 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     6241 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     6301 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     6361 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     6421 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     6481 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     6541 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     6601 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     6661 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     6721 ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
     6781 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
     6841 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
     6901 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
     6961 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
     7021 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     7081 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     7141 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     7201 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     7261 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     7321 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     7381 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     7441 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     7501 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
     7561 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     7621 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     7681 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     7741 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     7801 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     7861 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     7921 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     7981 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     8041 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     8101 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     8161 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     8221 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     8281 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     8341 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     8401 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     8461 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     8521 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     8581 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     8641 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     8701 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     8761 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     8821 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     8881 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     8941 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     9001 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     9061 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
     9121 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
     9181 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     9241 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     9301 ggacagcaga gatccagttt ggt
//
