LOCUS       sequence-352065-       10637 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible expression of Human ZNF267 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10637)
  AUTHORS   Rosa FF, Pires CF, Kurochkin I, Halitzki E, Zahan T, Arh N, 
            Zimmermannova O, Ferreira AG, Li H, Karlsson S, Scheding S, Pereira 
            CF
  TITLE     Single-cell transcriptional profiling informs efficient 
            reprogramming of human somatic cells to cross-presenting dendritic 
            cells.
  JOURNAL   Sci Immunol. 2022 Mar 4;7(69):eabg5539. doi: 
            10.1126/sciimmunol.abg5539. Epub 2022 Mar 4.
  PUBMED    35245086
REFERENCE   2  (bases 1 to 10637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2022-03-4"; volume: "7(69):eabg5539. doi"; pages: 
            " 10.1126/sciimmunol.abg5539. Epub 2022 Mar 4"
COMMENT     Sequence Label: pFUW-TetO-ZNF267
FEATURES             Location/Qualifiers
     source          1..10637
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    join(20..38,39..61,62..80,81..103,104..122,123..145,
                     146..164,165..187,188..206,207..229,230..248,249..271,
                     272..290)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        323..361
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     358..382
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    2735..3323
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2788..2808)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3206..3217)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3326..3342)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3327..3343)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3848..4028
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4054..4071)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4060..4284
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4330..4758
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4417..4436)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4627..4648
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4767..4787)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4772..5101
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4952..5087
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5014..5033
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5149..5196
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5215..5589
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5719..5840
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5756..5775)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5810..5829
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5889..5905)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5889..5905)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5902..5924)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5913..5929
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5937..5943,5944..5961,5962..5967))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5982..6003
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6120..6137)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6291..6879)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6371..6390)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7050..7841,7842..7910))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7673..7692
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7911..8015)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8090..8109)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8281..8660
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8662..8860
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             8878..9058
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9105..9230
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9723..9956
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10141..10185
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10483..10600
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tagatctcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
       61 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
      121 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
      181 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
      241 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
      301 tcgagctcgg tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc
      361 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
      421 aagacaccgg gaccgatcca gcctccgcgg ccccgaattc accggttcta gagctagcat
      481 gggactgttg acattcaggg atgtggccgt agaattctct ttggaggagt gggaacacct
      541 ggaaccagct cagaagaatt tgtatcagaa tgtgatgtta gaaaactaca gaaacctggt
      601 ctctctgggt cttgttgtct ctaagccgga cctgatcacc tttttggaac aaaggaaaga
      661 gccttggaat gtgaagagtg aggagacagt agccatccag ccagatgtgt tttcgcatta
      721 taacaaggac ctgttgacag agcactgcac agaagcttca ttccaaaaag tgatatcgag
      781 gagacatggg agctgtgatc ttgagaattt acatttaaga aaaaggtgga aaagggagga
      841 gtgtgaaggg cacaatggat gttatgatga aaagactttt aaatatgatc aatttgatga
      901 atcctctgtt gaaagtttgt ttcaccagca aatactttct tcttgtgcca aaagctataa
      961 ctttgatcaa tataggaagg tctttactca ttcatcattg cttaatcaac aagaggaaat
     1021 agatatttgg ggaaaacatc acatatatga taaaacttca gtgttattta ggcaggtctc
     1081 tactctaaat agttaccgaa atgtttttat tggagagaaa aattatcatt gcaataattc
     1141 tgaaaaaacc ttgaaccaaa gctcaagccc taaaaatcat caggaaaatt attttctaga
     1201 aaaacaatac aaatgtaaag aatttgagga agtctttctt cagagtatgc atgggcaaga
     1261 gaaacaagaa cagtcttaca aatgtaataa atgtgtagaa gtttgtaccc agtcattaaa
     1321 acatattcaa catcagacca tccatatcag agaaaactca tatagctata acaaatatga
     1381 taaagatctt agtcagtcat caaatcttag aaagcagata atccataatg aagagaaacc
     1441 atacaaatgt gaaaaatgtg gggatagctt aaaccatagt ttgcacctta ctcaacatca
     1501 gatcattcct accgaagaga aaccctataa atggaaagaa tgtggcaagg tctttaacct
     1561 taactgtagt ttatacctta ctaaacagca gcaaattgat actggagaaa acctttacaa
     1621 atgtaaagca tgtagcaaat cttttactcg ttcctccaat cttattgtgc atcagagaat
     1681 tcacactgga gagaaaccat acaaatgtaa agaatgtggc aaagcctttc gctgtagttc
     1741 ataccttact aaacataagc gaattcatac tggagagaaa ccttataaat gtaaagaatg
     1801 tggaaaagct tttaaccgta gttcatgcct tactcaacat cagacaactc atacaggaga
     1861 aaaactttac aaatgtaaag tatgtagcaa atcttatgct cgttcttcaa atcttattat
     1921 gcatcagaga gttcatactg gagagaagcc ttataaatgt aaagaatgtg gcaaagtctt
     1981 tagccgtagt tcttgcctta ctcaacatcg gaaaattcat actggagaaa atctttacaa
     2041 atgcaaagta tgtgctaaac cttttacttg tttctcaaat cttattgtgc atgagagaat
     2101 tcatactgga gagaaaccct ataaatgtaa agaatgtggc aaagcctttc cttatagttc
     2161 acaccttatt cgacatcatc gaattcatac tggagaaaaa ccatacaaat gtaaagcatg
     2221 tagcaaatct tttagtgact cctcaggtct tactgtgcat cggcgaactc atactggaga
     2281 gaaaccctat acatgtaaag aatgtggcaa agcctttagt tatagttcag atgttattca
     2341 gcatcggaga attcatactg gccagagacc ctacaaatgt gaagaatgtg gcaaagcctt
     2401 caactatagg tcatacctca ctacacatca gagaagtcat actggagaga gaccctacaa
     2461 atgtgaagaa tgtggcaaag ccttcaactc taggtcatac ctcactacac atcggagaag
     2521 acatactgga gagagaccct acaaatgtga tgaatgtggt aaagccttca gctataggtc
     2581 atacctcact acacatcgga gaagtcatag tggagagaga ccctacaaat gtgaagaatg
     2641 tggcaaagcc tttaactcta ggtcatacct cattgcacat cagagaagtc atactagaga
     2701 aaaactttaa ttcgaagata tcaagcttat cgataatcaa cctctggatt acaaaatttg
     2761 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     2821 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     2881 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     2941 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     3001 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     3061 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     3121 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     3181 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     3241 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     3301 ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagacctag aaaaacatgg
     3361 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca
     3421 agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta
     3481 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     3541 tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt
     3601 ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga cctttggatg
     3661 gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa
     3721 cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt
     3781 agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga
     3841 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     3901 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     3961 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     4021 ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     4081 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     4141 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     4201 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     4261 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     4321 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     4381 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     4441 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     4501 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     4561 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     4621 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     4681 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     4741 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     4801 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     4861 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     4921 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     4981 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     5041 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     5101 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa
     5161 tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc
     5221 aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc
     5281 tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc
     5341 cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc
     5401 ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg
     5461 tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg
     5521 gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag
     5581 caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     5641 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     5701 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     5761 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     5821 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     5881 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     5941 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     6001 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     6061 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     6121 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     6181 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     6241 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6301 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     6361 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     6421 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     6481 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     6541 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     6601 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     6661 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     6721 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     6781 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     6841 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     6901 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     6961 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7021 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     7081 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     7141 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     7201 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     7261 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     7321 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     7381 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     7441 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     7501 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     7561 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     7621 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     7681 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     7741 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     7801 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     7861 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     7921 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     7981 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     8041 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
     8101 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     8161 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     8221 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
     8281 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     8341 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     8401 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     8461 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     8521 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     8581 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     8641 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     8701 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     8761 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     8821 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
     8881 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     8941 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     9001 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     9061 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     9121 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     9181 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     9241 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     9301 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     9361 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     9421 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     9481 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     9541 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     9601 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     9661 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     9721 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     9781 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     9841 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     9901 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     9961 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
    10021 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
    10081 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
    10141 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
    10201 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
    10261 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
    10321 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
    10381 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
    10441 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
    10501 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
    10561 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
    10621 cagagatcca gtttggt
//
