LOCUS       sequence-352068-        9458 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible expression of Human PLEK in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9458)
  AUTHORS   Rosa FF, Pires CF, Kurochkin I, Halitzki E, Zahan T, Arh N, 
            Zimmermannova O, Ferreira AG, Li H, Karlsson S, Scheding S, Pereira 
            CF
  TITLE     Single-cell transcriptional profiling informs efficient 
            reprogramming of human somatic cells to cross-presenting dendritic 
            cells.
  JOURNAL   Sci Immunol. 2022 Mar 4;7(69):eabg5539. doi: 
            10.1126/sciimmunol.abg5539. Epub 2022 Mar 4.
  PUBMED    35245086
REFERENCE   2  (bases 1 to 9458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2022-03-4"; volume: "7(69):eabg5539. doi"; pages: 
            " 10.1126/sciimmunol.abg5539. Epub 2022 Mar 4"
COMMENT     Sequence Label: pFUW-TetO-PLEK
FEATURES             Location/Qualifiers
     source          1..9458
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    join(20..38,39..61,62..80,81..103,104..122,123..145,
                     146..164,165..187,188..206,207..229,230..248,249..271,
                     272..290)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        323..361
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     358..382
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    1556..2144
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1609..1629)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2027..2038)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2147..2163)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2148..2164)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2669..2849
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2875..2892)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2881..3105
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3151..3579
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3238..3257)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3448..3469
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3588..3608)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3593..3922
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3773..3908
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3835..3854
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3970..4017
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4036..4410
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4540..4661
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4577..4596)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4631..4650
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4710..4726)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4710..4726)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4723..4745)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4734..4750
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4758..4764,4765..4782,4783..4788))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4803..4824
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4941..4958)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5112..5700)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5192..5211)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5871..6662,6663..6731))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6494..6513
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6732..6836)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6911..6930)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7102..7481
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7483..7681
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             7699..7879
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7926..8051
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8544..8777
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8962..9006
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9304..9421
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tagatctcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
       61 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
      121 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
      181 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
      241 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
      301 tcgagctcgg tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc
      361 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
      421 aagacaccgg gaccgatcca gcctccgcgg ccccgaattc accggttcta gagctagcat
      481 ggaaccaaag cggatcagag agggctacct tgtgaagaag gggagcgtgt tcaatacgtg
      541 gaaacccatg tgggttgtat tgttagaaga tggaattgaa ttctataaga agaaaagtga
      601 caacagcccc aaaggaatga tcccgctgaa agggagcact ctgactagcc cttgtcaaga
      661 ctttggcaaa aggatgtttg tgtttaagat cactacgacc aaacagcagg accacttctt
      721 ccaggcagcc ttcctggagg agagagatgc ctgggttcgg gatatcaaga aggccattaa
      781 atgcattgaa ggaggccaga aatttgccag gaaatctacc aggaggtcca ttcgactgcc
      841 agaaaccatt gacttaggtg ccttatattt gtccatgaaa gacactgaaa aaggaataaa
      901 agaactgaat ctagagaagg acaagaagat ttttaatcac tgcttcacag gtaactgcgt
      961 cattgattgg ctggtatcca accagtctgt taggaatcgc caggaaggcc tcatgattgc
     1021 ttcatcgctg ctcaatgagg ggtatctgca gcctgctgga gacatgtcca agagtgcagt
     1081 ggatggaact gctgaaaacc ctttcctgga caaccctgat gccttctact actttccaga
     1141 cagtgggttc ttctgtgaag agaattccag tgatgatgat gtgattctga aagaagaatt
     1201 cagaggggtc attatcaagc agggatgttt actgaagcag gggcatagaa ggaaaaactg
     1261 gaaagtgagg aagttcatct tgagagaaga ccctgcctac ctgcactact atgaccctgc
     1321 tggggcagaa gatcccctgg gagcaattca cttgagaggc tgtgtggtga cttcagtgga
     1381 gagcaactca aatggcagga agagtgagga agagaacctt tttgagatca tcacagcaga
     1441 tgaagtgcac tatttcttgc aagcagccac ccccaaggag cgcacagagt ggatcaaagc
     1501 catccagatg gcctcccgaa ctgggaagta attcgaagat atcaagctta tcgataatca
     1561 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     1621 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     1681 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     1741 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     1801 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     1861 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     1921 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     1981 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     2041 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     2101 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc
     2161 tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga
     2221 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt
     2281 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     2341 ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat
     2401 ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc cagggatcag
     2461 atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga
     2521 agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga
     2581 tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat
     2641 ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg
     2701 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     2761 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     2821 cttttagtca gtgtggaaaa tctctagcag ggcccgttta aacccgctga tcagcctcga
     2881 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     2941 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     3001 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     3061 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     3121 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     3181 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     3241 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     3301 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     3361 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     3421 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     3481 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     3541 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     3601 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     3661 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     3721 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     3781 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     3841 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     3901 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     3961 tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag
     4021 gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac
     4081 gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag
     4141 gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac
     4201 caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac
     4261 gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc
     4321 gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc
     4381 gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc
     4441 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     4501 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     4561 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     4621 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     4681 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     4741 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     4801 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     4861 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     4921 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     4981 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     5041 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     5101 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     5161 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     5221 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     5281 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     5341 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     5401 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     5461 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     5521 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     5581 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     5641 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     5701 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     5761 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     5821 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     5881 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     5941 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     6001 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     6061 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     6121 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     6181 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     6241 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     6301 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     6361 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     6421 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     6481 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     6541 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     6601 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     6661 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     6721 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     6781 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     6841 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
     6901 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     6961 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     7021 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
     7081 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
     7141 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     7201 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     7261 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     7321 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     7381 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     7441 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     7501 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     7561 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
     7621 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     7681 gcgcgttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     7741 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     7801 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     7861 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     7921 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     7981 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     8041 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     8101 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     8161 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     8221 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     8281 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     8341 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
     8401 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     8461 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     8521 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     8581 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     8641 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     8701 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     8761 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     8821 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     8881 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     8941 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     9001 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     9061 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     9121 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     9181 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga
     9241 ttagtgaacg gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca
     9301 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     9361 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     9421 tcgggtttat tacagggaca gcagagatcc agtttggt
//
