LOCUS       sequence-35208-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Takaki K, Davis JM, Winglee K, Ramakrishnan L
  TITLE     Evaluation of the pathogenesis and treatment of Mycobacterium 
            marinum infection in zebrafish.
  JOURNAL   Nat Protoc. 2013 Jun;8(6):1114-24. doi: 10.1038/nprot.2013.068. Epub
            2013 May 16.
  PUBMED    23680983
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2013.068"; journalName: "Nat Protoc"; date: 
            "2013-06"; volume: "8"; issue: "6"; pages: "1114-24"
COMMENT     Sequence Label: pTEC27
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(537..553)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(538..554)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(741..759)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 tggcagtcag agcgaccagg ccgaccgata cgaggcggat tttgaacagg ttcactcgaa
       61 gcctcccgct gggttcggag catcgtcgat cacatgacgg cgtgctgaca ccgattcgac
      121 tatgcacagt agtaggtagc cgccgcggac ggtagcgcag gcggcctcgg cgccgtcgtg
      181 gcgtcgcgat ggttggcgcc agacccgcct gatcggcgtc ccgaccaggg ccgccggggt
      241 ggtgagcctc ggcttggtcc agccgtagtg agacgggatg tcttcccggt tgtcgggttt
      301 gggtgggcgg tcacatgttc gctggctgtg ggcgtgtcgg gggtcgtcga agcggccgcg
      361 aacagggctg gacgatgcac gtcttgcacc tcctgtcaac ccccaatgtt gagccgcgat
      421 gcccctgacc tgcaccgttg gaccgcagtc gttagggggg cgtgttagca tggagtgacg
      481 aaaggggcct aaatcagatg atcccccggg ctgcaggaat tcgatatcaa gcttatcgat
      541 accgtcgacc tcgagggggg gcccggtacc agatctttaa atctagattt aagaaggaga
      601 tatacatatg gtgagcaagg gcgaggaggt catcaaagag ttcatgcgct tcaaggtgcg
      661 catggagggc tccatgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
      721 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc
      781 ctgggacatc ctgtcccccc agttcatgta cggctccaa
//
