LOCUS       sequence-352085-        6609 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sars-CoV-2 protein mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6609)
  AUTHORS   Liu X, Huuskonen S, Laitinen T, Redchuk T, Bogacheva M, Salokas K, 
            Pohner I, Ohman T, Tonduru AK, Hassinen A, Gawriyski L, Keskitalo S,
            Vartiainen MK, Pietiainen V, Poso A, Varjosalo M
  TITLE     SARS-CoV-2-host proteome interactions for antiviral drug discovery.
  JOURNAL   Mol Syst Biol. 2021 Nov;17(11):e10396. doi: 10.15252/msb.202110396.
  PUBMED    34709727
REFERENCE   2  (bases 1 to 6609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2021-11"; volume: "17(11):e10396. doi"; pages: " 
            10.15252/msb.202110396"
COMMENT     Sequence Label: pDON223 Spike A701V
FEATURES             Location/Qualifiers
     source          1..6609
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(130..157)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(249..335)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     384..406
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     398..415
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     399..415
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    431..530
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4350..4449)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4466..4485)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4467..4485)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4490..4506)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4490..4506)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4937..5728
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5821..6409
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6310..6329
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6563..6580
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
       61 gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc gctagccagg aagagtttgt
      121 agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt
      181 tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct
      241 cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag
      301 gcccagtctt ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc
      361 gttaacgcta gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa
      421 gctcgggccc caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat
      481 tgatgagcaa tgctttttta taatgccaac tttgtacaaa aaagttggca tgttcgtgtt
      541 cctggtgctg ctgccactgg tgagctccca gtgcgtgaac ctgaccacca gaacccagct
      601 gccacctgcc tacaccaact ccttcaccag gggcgtgtac tacccagaca aggtgttcag
      661 atccagcgtg ctgcactcta cccaggacct gttcctgcca ttcttctcta acgtgacctg
      721 gttccacgcc atccacgtgt ctggaaccaa cggcaccaag agattcgaca acccagtgct
      781 gccattcaac gacggcgtgt acttcgcttc taccgagaag tctaacatca tcaggggttg
      841 gatcttcgga accaccctgg actctaagac ccagtctctg ctgatcgtga acaacgctac
      901 caacgtggtg atcaaggtgt gcgagttcca gttctgcaac gacccattcc tgggagtgta
      961 ctaccacaag aacaacaagt cttggatgga aagcgagttc agggtgtaca gctctgctaa
     1021 caactgcacc ttcgagtacg tgtctcagcc tttcctgatg gacctggaag gaaagcaggg
     1081 aaacttcaag aacctgagag agttcgtgtt caagaacatc gacggttact tcaagatata
     1141 cagcaagcac accccaatca acctggtgag ggacctgcca cagggtttct ctgctctgga
     1201 gcccctggtg gacctgccaa tcggcatcaa catcaccagg ttccagaccc tgctggctct
     1261 gcacaggtct tacctgaccc caggcgacag cagctctgga tggaccgccg gcgccgctgc
     1321 ttactacgtg ggatacctgc agccaaggac cttcctgctg aagtacaacg aaaacggcac
     1381 catcaccgac gccgtggact gcgctctgga cccactgtct gagaccaagt gcaccctgaa
     1441 gtctttcacc gtggaaaagg gaatctacca gaccagcaac ttccgcgtgc agcccaccga
     1501 gtctatcgtg agattcccaa acatcaccaa cctgtgccca ttcggagaag tgttcaacgc
     1561 taccaggttc gccagcgtgt acgcttggaa caggaagaga atcagcaact gcgtggccga
     1621 ctactctgtg ctgtacaact ctgcctcctt ctccaccttc aagtgctacg gcgtgtcccc
     1681 aaccaagctg aacgacctgt gcttcaccaa cgtgtacgct gactccttcg tgatcagagg
     1741 agacgaagtg agacagatcg ctcccggtca gaccggaaag atcgccgact acaactacaa
     1801 gctgccagac gacttcaccg gttgcgtgat cgcttggaac tctaacaacc tggactctaa
     1861 ggtgggaggc aactacaact acctgtacag gctgttccgc aagagcaacc tgaagccttt
     1921 cgaaagggac atcagcaccg aaatctacca ggctggatct accccatgca acggagtgga
     1981 aggattcaac tgctacttcc ctctgcagtc ttacggattc cagccaacca acggagtggg
     2041 ataccagcca tacagagtgg tggtgctgtc tttcgagctg ctgcacgctc ctgctaccgt
     2101 gtgcggacca aagaagtcta ccaacctggt gaagaacaag tgcgtgaact tcaacttcaa
     2161 cggactgacc ggaaccggag tgctgaccga atccaacaag aagttcctgc ctttccagca
     2221 gttcggtaga gacatcgccg acaccaccga cgctgtgaga gacccacaga ccctggaaat
     2281 cctggacatc accccctgct ctttcggagg agtgagcgtg atcaccccag gaaccaacac
     2341 ctccaaccag gtggccgtgc tgtaccagga cgtgaactgc accgaagtgc cagtggccat
     2401 ccacgctgac cagctgaccc caacctggag agtgtacagc accggatcta acgtgttcca
     2461 gacccgtgcc ggctgcctga tcggagccga gcacgtgaac aactcctacg aatgcgacat
     2521 cccaatcgga gctggcatct gcgcttctta ccagacccag accaacagcc caagaagggc
     2581 cagatccgtg gcctctcaga gcatcatcgc ctacaccatg agcctgggcg ttgaaaacag
     2641 cgtggcttac agcaacaact ctatcgccat cccaaccaac ttcaccatct ctgtgaccac
     2701 cgaaatcctg ccagtgagca tgaccaagac cagcgtggac tgcaccatgt acatctgcgg
     2761 agactctacc gaatgctcta acctgctgct gcagtacggc agcttctgca cccagctgaa
     2821 ccgcgctctg accggaatcg ctgtggagca ggacaagaac acccaggaag tgttcgctca
     2881 ggtgaagcag atatacaaga ccccacctat caaggacttc ggaggattca acttctctca
     2941 gatcctgccc gacccatcta agccctctaa gaggtctttc atcgaagacc tgctgttcaa
     3001 caaggtgacc ctggccgacg ccggtttcat caagcagtac ggtgactgcc tgggagacat
     3061 cgccgctaga gacctgatct gcgcccagaa gttcaacggt ctgaccgtgc tgccaccact
     3121 gctgaccgac gagatgatcg cccagtacac ctctgccctg ctggctggaa ccatcacctc
     3181 tggttggacc ttcggagctg gcgccgccct gcagatccca ttcgctatgc agatggccta
     3241 ccgtttcaac ggtatcggag tgacccagaa cgtgctgtac gaaaaccaga agctgatcgc
     3301 taaccagttc aactctgcta tcggaaagat ccaggactcc ctgtcctcta ccgcttctgc
     3361 cctgggaaag ctgcaggacg tggtgaacca gaacgcccag gccctgaaca ccctggtgaa
     3421 gcagctgtct tctaacttcg gagccatctc ctctgtgctg aacgacatcc tgtctaggct
     3481 ggacaaggtg gaagccgagg tgcagatcga cagactgatc accggaagac tgcagtctct
     3541 gcagacctac gtgacccagc agctgatcag ggccgctgaa atcagagctt ctgccaacct
     3601 ggctgccacc aagatgtccg aatgcgtgct gggccagtct aagagggtgg acttctgcgg
     3661 aaagggatac cacctgatgt ccttcccaca gtctgctcca cacggagtgg tgttcctgca
     3721 cgtgacctac gtgcccgccc aggaaaagaa cttcaccacc gccccagcta tctgccacga
     3781 cggaaaggct cacttcccca gggagggagt gttcgtgtct aacggaaccc actggttcgt
     3841 gacccagagg aacttctacg agccacagat catcaccacc gacaacacct tcgtgtctgg
     3901 aaactgcgac gtggtgatcg gaatcgtgaa caacaccgtg tacgacccac tgcagccaga
     3961 actggactct ttcaaggagg aactggacaa gtacttcaag aaccacacct ctccagacgt
     4021 ggacctggga gacatctccg gaatcaacgc ctctgtggtg aacatccaga aggaaatcga
     4081 cagactgaac gaggtggcca agaacctgaa cgaatctctg atcgacctgc aggaactggg
     4141 aaagtacgag cagtacatca agtggccctg gtacatctgg ctgggtttca tcgctggtct
     4201 gatcgctatc gtgatggtga ccatcatgct gtgctgcatg acctcttgct gctcttgcct
     4261 gaagggatgc tgctcttgcg gatcttgctg caagttcgac gaggacgact ccgagcctgt
     4321 gctgaagggt gtgaagctgc actacaccta cccaactttc ttgtacaaag ttggcattat
     4381 aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
     4441 cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt
     4501 ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
     4561 tatatcatca tgcctcctct agaccagcca ggacagaaat gcctcgactt cgctgctacc
     4621 caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc agtggacata
     4681 agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag
     4741 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
     4801 ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt
     4861 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa
     4921 aacaaagtta aacattatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga
     4981 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg
     5041 ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac
     5101 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc
     5161 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga
     5221 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa
     5281 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct
     5341 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga
     5401 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc
     5461 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta
     5521 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg
     5581 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga
     5641 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg
     5701 cgagatcacc aaggtagtcg gcaaataacc ctcgagccac ccatgaccaa aatcccttaa
     5761 cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     5821 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     5881 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     5941 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     6001 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     6061 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     6121 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     6181 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     6241 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     6301 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     6361 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     6421 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     6481 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     6541 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     6601 acgcaaacc
//
