LOCUS       sequence-352088-        6609 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sars-CoV-2 protein mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6609)
  AUTHORS   Liu X, Huuskonen S, Laitinen T, Redchuk T, Bogacheva M, Salokas K, 
            Pohner I, Ohman T, Tonduru AK, Hassinen A, Gawriyski L, Keskitalo S,
            Vartiainen MK, Pietiainen V, Poso A, Varjosalo M
  TITLE     SARS-CoV-2-host proteome interactions for antiviral drug discovery.
  JOURNAL   Mol Syst Biol. 2021 Nov;17(11):e10396. doi: 10.15252/msb.202110396.
  PUBMED    34709727
REFERENCE   2  (bases 1 to 6609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2021-11"; volume: "17(11):e10396. doi"; pages: " 
            10.15252/msb.202110396"
COMMENT     Sequence Label: pDON223 Spike K417N
FEATURES             Location/Qualifiers
     source          1..6609
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     123..142
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     376..393
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(552..579)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(671..757)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     806..828
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     820..837
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     821..837
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    853..952
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4772..4871)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4888..4907)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4889..4907)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4912..4928)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4912..4928)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             5359..6150
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(6243..6609,1..222)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
       61 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
      121 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
      181 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
      241 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
      301 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
      361 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
      421 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
      481 tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt
      541 gtagaaacgc aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag
      601 tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg
      661 ctcccggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa
      721 aggcccagtc ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc
      781 gcgttaacgc tagcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt
      841 aagctcgggc cccaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa
      901 attgatgagc aatgcttttt tataatgcca actttgtaca aaaaagttgg catgttcgtg
      961 ttcctggtgc tgctgccact ggtgagctcc cagtgcgtga acctgaccac cagaacccag
     1021 ctgccacctg cctacaccaa ctccttcacc aggggcgtgt actacccaga caaggtgttc
     1081 agatccagcg tgctgcactc tacccaggac ctgttcctgc cattcttctc taacgtgacc
     1141 tggttccacg ccatccacgt gtctggaacc aacggcacca agagattcga caacccagtg
     1201 ctgccattca acgacggcgt gtacttcgct tctaccgaga agtctaacat catcaggggt
     1261 tggatcttcg gaaccaccct ggactctaag acccagtctc tgctgatcgt gaacaacgct
     1321 accaacgtgg tgatcaaggt gtgcgagttc cagttctgca acgacccatt cctgggagtg
     1381 tactaccaca agaacaacaa gtcttggatg gaaagcgagt tcagggtgta cagctctgct
     1441 aacaactgca ccttcgagta cgtgtctcag cctttcctga tggacctgga aggaaagcag
     1501 ggaaacttca agaacctgag agagttcgtg ttcaagaaca tcgacggtta cttcaagata
     1561 tacagcaagc acaccccaat caacctggtg agggacctgc cacagggttt ctctgctctg
     1621 gagcccctgg tggacctgcc aatcggcatc aacatcacca ggttccagac cctgctggct
     1681 ctgcacaggt cttacctgac cccaggcgac agcagctctg gatggaccgc cggcgccgct
     1741 gcttactacg tgggatacct gcagccaagg accttcctgc tgaagtacaa cgaaaacggc
     1801 accatcaccg acgccgtgga ctgcgctctg gacccactgt ctgagaccaa gtgcaccctg
     1861 aagtctttca ccgtggaaaa gggaatctac cagaccagca acttccgcgt gcagcccacc
     1921 gagtctatcg tgagattccc aaacatcacc aacctgtgcc cattcggaga agtgttcaac
     1981 gctaccaggt tcgccagcgt gtacgcttgg aacaggaaga gaatcagcaa ctgcgtggcc
     2041 gactactctg tgctgtacaa ctctgcctcc ttctccacct tcaagtgcta cggcgtgtcc
     2101 ccaaccaagc tgaacgacct gtgcttcacc aacgtgtacg ctgactcctt cgtgatcaga
     2161 ggagacgaag tgagacagat cgctcccggt cagaccggaa atatcgccga ctacaactac
     2221 aagctgccag acgacttcac cggttgcgtg atcgcttgga actctaacaa cctggactct
     2281 aaggtgggag gcaactacaa ctacctgtac aggctgttcc gcaagagcaa cctgaagcct
     2341 ttcgaaaggg acatcagcac cgaaatctac caggctggat ctaccccatg caacggagtg
     2401 gaaggattca actgctactt ccctctgcag tcttacggat tccagccaac caacggagtg
     2461 ggataccagc catacagagt ggtggtgctg tctttcgagc tgctgcacgc tcctgctacc
     2521 gtgtgcggac caaagaagtc taccaacctg gtgaagaaca agtgcgtgaa cttcaacttc
     2581 aacggactga ccggaaccgg agtgctgacc gaatccaaca agaagttcct gcctttccag
     2641 cagttcggta gagacatcgc cgacaccacc gacgctgtga gagacccaca gaccctggaa
     2701 atcctggaca tcaccccctg ctctttcgga ggagtgagcg tgatcacccc aggaaccaac
     2761 acctccaacc aggtggccgt gctgtaccag gacgtgaact gcaccgaagt gccagtggcc
     2821 atccacgctg accagctgac cccaacctgg agagtgtaca gcaccggatc taacgtgttc
     2881 cagacccgtg ccggctgcct gatcggagcc gagcacgtga acaactccta cgaatgcgac
     2941 atcccaatcg gagctggcat ctgcgcttct taccagaccc agaccaacag cccaagaagg
     3001 gccagatccg tggcctctca gagcatcatc gcctacacca tgagcctggg cgctgaaaac
     3061 agcgtggctt acagcaacaa ctctatcgcc atcccaacca acttcaccat ctctgtgacc
     3121 accgaaatcc tgccagtgag catgaccaag accagcgtgg actgcaccat gtacatctgc
     3181 ggagactcta ccgaatgctc taacctgctg ctgcagtacg gcagcttctg cacccagctg
     3241 aaccgcgctc tgaccggaat cgctgtggag caggacaaga acacccagga agtgttcgct
     3301 caggtgaagc agatatacaa gaccccacct atcaaggact tcggaggatt caacttctct
     3361 cagatcctgc ccgacccatc taagccctct aagaggtctt tcatcgaaga cctgctgttc
     3421 aacaaggtga ccctggccga cgccggtttc atcaagcagt acggtgactg cctgggagac
     3481 atcgccgcta gagacctgat ctgcgcccag aagttcaacg gtctgaccgt gctgccacca
     3541 ctgctgaccg acgagatgat cgcccagtac acctctgccc tgctggctgg aaccatcacc
     3601 tctggttgga ccttcggagc tggcgccgcc ctgcagatcc cattcgctat gcagatggcc
     3661 taccgtttca acggtatcgg agtgacccag aacgtgctgt acgaaaacca gaagctgatc
     3721 gctaaccagt tcaactctgc tatcggaaag atccaggact ccctgtcctc taccgcttct
     3781 gccctgggaa agctgcagga cgtggtgaac cagaacgccc aggccctgaa caccctggtg
     3841 aagcagctgt cttctaactt cggagccatc tcctctgtgc tgaacgacat cctgtctagg
     3901 ctggacaagg tggaagccga ggtgcagatc gacagactga tcaccggaag actgcagtct
     3961 ctgcagacct acgtgaccca gcagctgatc agggccgctg aaatcagagc ttctgccaac
     4021 ctggctgcca ccaagatgtc cgaatgcgtg ctgggccagt ctaagagggt ggacttctgc
     4081 ggaaagggat accacctgat gtccttccca cagtctgctc cacacggagt ggtgttcctg
     4141 cacgtgacct acgtgcccgc ccaggaaaag aacttcacca ccgccccagc tatctgccac
     4201 gacggaaagg ctcacttccc cagggaggga gtgttcgtgt ctaacggaac ccactggttc
     4261 gtgacccaga ggaacttcta cgagccacag atcatcacca ccgacaacac cttcgtgtct
     4321 ggaaactgcg acgtggtgat cggaatcgtg aacaacaccg tgtacgaccc actgcagcca
     4381 gaactggact ctttcaagga ggaactggac aagtacttca agaaccacac ctctccagac
     4441 gtggacctgg gagacatctc cggaatcaac gcctctgtgg tgaacatcca gaaggaaatc
     4501 gacagactga acgaggtggc caagaacctg aacgaatctc tgatcgacct gcaggaactg
     4561 ggaaagtacg agcagtacat caagtggccc tggtacatct ggctgggttt catcgctggt
     4621 ctgatcgcta tcgtgatggt gaccatcatg ctgtgctgca tgacctcttg ctgctcttgc
     4681 ctgaagggat gctgctcttg cggatcttgc tgcaagttcg acgaggacga ctccgagcct
     4741 gtgctgaagg gtgtgaagct gcactacacc tacccaactt tcttgtacaa agttggcatt
     4801 ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
     4861 atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct
     4921 gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa
     4981 aatatatcat catgcctcct ctagaccagc caggacagaa atgcctcgac ttcgctgcta
     5041 cccaaggttg ccgggtgacg cacaccgtgg aaacggatga aggcacgaac ccagtggaca
     5101 taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc
     5161 agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat
     5221 gactgttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc
     5281 gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct
     5341 aaaacaaagt taaacattat gagggaagcg gtgatcgccg aagtatcgac tcaactatca
     5401 gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac
     5461 ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg
     5521 accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg
     5581 gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac
     5641 gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc
     5701 aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg
     5761 ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt
     5821 gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac
     5881 tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg
     5941 tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag
     6001 cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa
     6061 gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa
     6121 ggcgagatca ccaaggtagt cggcaaataa ccctcgagcc acccatgacc aaaatccctt
     6181 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     6241 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     6301 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     6361 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     6421 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     6481 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6541 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     6601 acctacacc
//
