LOCUS       sequence-352091-        6609 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sars-CoV-2 protein mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6609)
  AUTHORS   Liu X, Huuskonen S, Laitinen T, Redchuk T, Bogacheva M, Salokas K, 
            Pohner I, Ohman T, Tonduru AK, Hassinen A, Gawriyski L, Keskitalo S,
            Vartiainen MK, Pietiainen V, Poso A, Varjosalo M
  TITLE     SARS-CoV-2-host proteome interactions for antiviral drug discovery.
  JOURNAL   Mol Syst Biol. 2021 Nov;17(11):e10396. doi: 10.15252/msb.202110396.
  PUBMED    34709727
REFERENCE   2  (bases 1 to 6609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2021-11"; volume: "17(11):e10396. doi"; pages: " 
            10.15252/msb.202110396"
COMMENT     Sequence Label: pDON223 Spike T1027I
FEATURES             Location/Qualifiers
     source          1..6609
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     690..706
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     690..706
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     711..730
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        711..729
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    747..846
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4666..4765)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4781..4798)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4781..4797)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4790..4812)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      4861..4947
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5039..5066
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(5225..5242)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5396..5984)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5476..5495)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6077..6609,1..259))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ctacagcgcg gagaatctcg ctctctccag gggaagccga agtttccaaa aggtcgttga
       61 tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat
      121 cactgtgtgg cttcaggccg ccatccactg cggagccgta caaatgtacg gccagcaacg
      181 tcggttcgag atggcgctcg atgacgccaa ctacctctga tagttgagtc gatacttcgg
      241 cgatcaccgc ttccctcata atgtttaact ttgttttagg gcgactgccc tgctgcgtaa
      301 catcgttgct gctccataac atcaaacatc gacccacggc gtaacgcgct tgctgcttgg
      361 atgcccgagg catagactgt accccaaaaa aacagtcata acaagccatg aaaaccgcca
      421 ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac
      481 gctacttgca ttacagctta cgaaccgaac aggcttatgt ccactgggtt cgtgccttca
      541 tccgtttcca cggtgtgcgt cacccggcaa ccttgggtag cagcgaagtc gaggcatttc
      601 tgtcctggct ggtctagagg aggcatgatg atatattttt atcttgtgca atgtaacatc
      661 agagattttg agacacgggc cagagctgcc aggaaacagc tatgaccatg taatacgact
      721 cactataggg gatatcagct ggatggcaaa taatgatttt attttgactg atagtgacct
      781 gttcgttgca acaaattgat aagcaatgct ttcttataat gccaactttg tacaagaaag
      841 ttgggtaggt gtagtgcagc ttcacaccct tcagcacagg ctcggagtcg tcctcgtcga
      901 acttgcagca agatccgcaa gagcagcatc ccttcaggca agagcagcaa gaggtcatgc
      961 agcacagcat gatggtcacc atcacgatag cgatcagacc agcgatgaaa cccagccaga
     1021 tgtaccaggg ccacttgatg tactgctcgt actttcccag ttcctgcagg tcgatcagag
     1081 attcgttcag gttcttggcc acctcgttca gtctgtcgat ttccttctgg atgttcacca
     1141 cagaggcgtt gattccggag atgtctccca ggtccacgtc tggagaggtg tggttcttga
     1201 agtacttgtc cagttcctcc ttgaaagagt ccagttctgg ctgcagtggg tcgtacacgg
     1261 tgttgttcac gattccgatc accacgtcgc agtttccaga cacgaaggtg ttgtcggtgg
     1321 tgatgatctg tggctcgtag aagttcctct gggtcacgaa ccagtgggtt ccgttagaca
     1381 cgaacactcc ctccctgggg aagtgagcct ttccgtcgtg gcagatagct ggggcggtgg
     1441 tgaagttctt ttcctgggcg ggcacgtagg tcacgtgcag gaacaccact ccgtgtggag
     1501 cagactgtgg gaaggacatc aggtggtatc cctttccgca gaagtccacc ctcttagact
     1561 ggcccagcac gcattcggac atcttgatgg cagccaggtt ggcagaagct ctgatttcag
     1621 cggccctgat cagctgctgg gtcacgtagg tctgcagaga ctgcagtctt ccggtgatca
     1681 gtctgtcgat ctgcacctcg gcttccacct tgtccagcct agacaggatg tcgttcagca
     1741 cagaggagat ggctccgaag ttagaagaca gctgcttcac cagggtgttc agggcctggg
     1801 cgttctggtt caccacgtcc tgcagctttc ccagggcaga agcggtagag gacagggagt
     1861 cctggatctt tccgatagca gagttgaact ggttagcgat cagcttctgg ttttcgtaca
     1921 gcacgttctg ggtcactccg ataccgttga aacggtaggc catctgcata gcgaatggga
     1981 tctgcagggc ggcgccagct ccgaaggtcc aaccagaggt gatggttcca gccagcaggg
     2041 cagaggtgta ctgggcgatc atctcgtcgg tcagcagtgg tggcagcacg gtcagaccgt
     2101 tgaacttctg ggcgcagatc aggtctctag cggcgatgtc tcccaggcag tcaccgtact
     2161 gcttgatgaa accggcgtcg gccagggtca ccttgttgaa cagcaggtct tcgatgaaag
     2221 acctcttaga gggcttagat gggtcgggca ggatctgaga gaagttgaat cctccgaagt
     2281 ccttgatagg tggggtcttg tatatctgct tcacctgagc gaacacttcc tgggtgttct
     2341 tgtcctgctc cacagcgatt ccggtcagag cgcggttcag ctgggtgcag aagctgccgt
     2401 actgcagcag caggttagag cattcggtag agtctccgca gatgtacatg gtgcagtcca
     2461 cgctggtctt ggtcatgctc actggcagga tttcggtggt cacagagatg gtgaagttgg
     2521 ttgggatggc gatagagttg ttgctgtaag ccacgctgtt ttcagcgccc aggctcatgg
     2581 tgtaggcgat gatgctctga gaggccacgg atctggccct tcttgggctg ttggtctggg
     2641 tctggtaaga agcgcagatg ccagctccga ttgggatgtc gcattcgtag gagttgttca
     2701 cgtgctcggc tccgatcagg cagccggcac gggtctggaa cacgttagat ccggtgctgt
     2761 acactctcca ggttggggtc agctggtcag cgtggatggc cactggcact tcggtgcagt
     2821 tcacgtcctg gtacagcacg gccacctggt tggaggtgtt ggttcctggg gtgatcacgc
     2881 tcactcctcc gaaagagcag ggggtgatgt ccaggatttc cagggtctgt gggtctctca
     2941 cagcgtcggt ggtgtcggcg atgtctctac cgaactgctg gaaaggcagg aacttcttgt
     3001 tggattcggt cagcactccg gttccggtca gtccgttgaa gttgaagttc acgcacttgt
     3061 tcttcaccag gttggtagac ttctttggtc cgcacacggt agcaggagcg tgcagcagct
     3121 cgaaagacag caccaccact ctgtatggct ggtatcccac tccgttggtt ggctggaatc
     3181 cgtaagactg cagagggaag tagcagttga atccttccac tccgttgcat ggggtagatc
     3241 cagcctggta gatttcggtg ctgatgtccc tttcgaaagg cttcaggttg ctcttgcgga
     3301 acagcctgta caggtagttg tagttgcctc ccaccttaga gtccaggttg ttagagttcc
     3361 aagcgatcac gcaaccggtg aagtcgtctg gcagcttgta gttgtagtcg gcgatctttc
     3421 cggtctgacc gggagcgatc tgtctcactt cgtctcctct gatcacgaag gagtcagcgt
     3481 acacgttggt gaagcacagg tcgttcagct tggttgggga cacgccgtag cacttgaagg
     3541 tggagaagga ggcagagttg tacagcacag agtagtcggc cacgcagttg ctgattctct
     3601 tcctgttcca agcgtacacg ctggcgaacc tggtagcgtt gaacacttct ccgaatgggc
     3661 acaggttggt gatgtttggg aatctcacga tagactcggt gggctgcacg cggaagttgc
     3721 tggtctggta gattcccttt tccacggtga aagacttcag ggtgcacttg gtctcagaca
     3781 gtgggtccag agcgcagtcc acggcgtcgg tgatggtgcc gttttcgttg tacttcagca
     3841 ggaaggtcct tggctgcagg tatcccacgt agtaagcagc ggcgccggcg gtccatccag
     3901 agctgctgtc gcctggggtc aggtaagacc tgtgcagagc cagcagggtc tggaacctgg
     3961 tgatgttgat gccgattggc aggtccacca ggggctccag agcagagaaa ccctgtggca
     4021 ggtccctcac caggttgatt ggggtgtgct tgctgtatat cttgaagtaa ccgtcgatgt
     4081 tcttgaacac gaactctctc aggttcttga agtttccctg ctttccttcc aggtccatca
     4141 ggaaaggctg agacacgtac tcgaaggtgc agttgttagc agagctgtac accctgaact
     4201 cgctttccat ccaagacttg ttgttcttgt ggtagtacac tcccaggaat gggtcgttgc
     4261 agaactggaa ctcgcacacc ttgatcacca cgttggtagc gttgttcacg atcagcagag
     4321 actgggtctt agagtccagg gtggttccga agatccaacc cctgatgatg ttagacttct
     4381 cggtagaagc gaagtacacg ccgtcgttga atggcagcac tgggttgtcg aatctcttgg
     4441 tgccgttggt tccagacacg tggatggcgt ggaaccaggt cacgttagag aagaatggca
     4501 ggaacaggtc ctgggtagag tgcagcacgc tggatctgaa caccttgtct gggtagtaca
     4561 cgcccctggt gaaggagttg gtgtaggcag gtggcagctg ggttctggtg gtcaggttca
     4621 cgcactggga gctcaccagt ggcagcagca ccaggaacac gaacatgcca acttttttgt
     4681 acaaagttgg cattataaaa aagcattgct catcaatttg ttgcaacgaa caggtcacta
     4741 tcagtcaaaa taaaatcatt atttggggcc cgagcttaag actggccgtc gttttacaac
     4801 gtcgtgactg ggaaaacatc catgctagcg ttaacgcgag agtagggaac tgccaggcat
     4861 caaataaaac gaaaggctca gtcggaagac tgggcctttc gttttatctg ttgtttgtcg
     4921 gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgtgaagcaa
     4981 cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggcatca aactaagcag
     5041 aaggccatcc tgacggatgg cctttttgcg tttctacaaa ctcttcctgg ctagcggtac
     5101 gcgtattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     5161 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     5221 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     5281 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     5341 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     5401 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     5461 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     5521 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     5581 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     5641 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     5701 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     5761 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     5821 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     5881 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     5941 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     6001 tttctacggg gtctgacgct cagtggaacg acgcgtaact cacgttaagg gattttggtc
     6061 atgggtggct cgagggttat ttgccgacta ccttggtgat ctcgcctttc acgtagtgga
     6121 caaattcttc caactgatct gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag
     6181 cctgtctagc ttcaagtatg acgggctgat actgggccgg caggcgctcc attgcccagt
     6241 cggcagcgac atccttcggc gcgattttgc cggttactgc gctgtaccaa atgcgggaca
     6301 acgtaagcac tacatttcgc tcatcgccag cccagtcggg cggcgagttc catagcgtta
     6361 aggtttcatt tagcgcctca aatagatcct gttcaggaac cggatcaaag agttcctccg
     6421 ccgctggacc taccaaggca acgctatgtt ctcttgcttt tgtcagcaag atagccagat
     6481 caatgtcgat cgtggctggc tcgaagatac ctgcaagaat gtcattgcgc tgccattctc
     6541 caaattgcag ttcgcgctta gctggataac gccacggaat gatgtcgtcg tgcacaacaa
     6601 tggtgactt
//
