LOCUS       sequence-352092-        6609 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sars-CoV-2 protein mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6609)
  AUTHORS   Liu X, Huuskonen S, Laitinen T, Redchuk T, Bogacheva M, Salokas K, 
            Pohner I, Ohman T, Tonduru AK, Hassinen A, Gawriyski L, Keskitalo S,
            Vartiainen MK, Pietiainen V, Poso A, Varjosalo M
  TITLE     SARS-CoV-2-host proteome interactions for antiviral drug discovery.
  JOURNAL   Mol Syst Biol. 2021 Nov;17(11):e10396. doi: 10.15252/msb.202110396.
  PUBMED    34709727
REFERENCE   2  (bases 1 to 6609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2021-11"; volume: "17(11):e10396. doi"; pages: " 
            10.15252/msb.202110396"
COMMENT     Sequence Label: pDON223 Spike S982A
FEATURES             Location/Qualifiers
     source          1..6609
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     83..102
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     336..353
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(512..539)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(631..717)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     766..788
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     780..797
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     781..797
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    813..912
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4732..4831)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4848..4867)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4849..4867)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4872..4888)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4872..4888)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             5319..6110
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(6203..6609,1..182)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
       61 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      121 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      181 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      241 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      301 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      361 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
      421 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta
      481 ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg
      541 cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg
      601 cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga
      661 caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt ttatttgatg
      721 cctggcagtt ccctactctc gcgttaacgc tagcatggat gttttcccag tcacgacgtt
      781 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
      841 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
      901 aaaaagttgg catgttcgtg ttcctggtgc tgctgccact ggtgagctcc cagtgcgtga
      961 acctgaccac cagaacccag ctgccacctg cctacaccaa ctccttcacc aggggcgtgt
     1021 actacccaga caaggtgttc agatccagcg tgctgcactc tacccaggac ctgttcctgc
     1081 cattcttctc taacgtgacc tggttccacg ccatccacgt gtctggaacc aacggcacca
     1141 agagattcga caacccagtg ctgccattca acgacggcgt gtacttcgct tctaccgaga
     1201 agtctaacat catcaggggt tggatcttcg gaaccaccct ggactctaag acccagtctc
     1261 tgctgatcgt gaacaacgct accaacgtgg tgatcaaggt gtgcgagttc cagttctgca
     1321 acgacccatt cctgggagtg tactaccaca agaacaacaa gtcttggatg gaaagcgagt
     1381 tcagggtgta cagctctgct aacaactgca ccttcgagta cgtgtctcag cctttcctga
     1441 tggacctgga aggaaagcag ggaaacttca agaacctgag agagttcgtg ttcaagaaca
     1501 tcgacggtta cttcaagata tacagcaagc acaccccaat caacctggtg agggacctgc
     1561 cacagggttt ctctgctctg gagcccctgg tggacctgcc aatcggcatc aacatcacca
     1621 ggttccagac cctgctggct ctgcacaggt cttacctgac cccaggcgac agcagctctg
     1681 gatggaccgc cggcgccgct gcttactacg tgggatacct gcagccaagg accttcctgc
     1741 tgaagtacaa cgaaaacggc accatcaccg acgccgtgga ctgcgctctg gacccactgt
     1801 ctgagaccaa gtgcaccctg aagtctttca ccgtggaaaa gggaatctac cagaccagca
     1861 acttccgcgt gcagcccacc gagtctatcg tgagattccc aaacatcacc aacctgtgcc
     1921 cattcggaga agtgttcaac gctaccaggt tcgccagcgt gtacgcttgg aacaggaaga
     1981 gaatcagcaa ctgcgtggcc gactactctg tgctgtacaa ctctgcctcc ttctccacct
     2041 tcaagtgcta cggcgtgtcc ccaaccaagc tgaacgacct gtgcttcacc aacgtgtacg
     2101 ctgactcctt cgtgatcaga ggagacgaag tgagacagat cgctcccggt cagaccggaa
     2161 agatcgccga ctacaactac aagctgccag acgacttcac cggttgcgtg atcgcttgga
     2221 actctaacaa cctggactct aaggtgggag gcaactacaa ctacctgtac aggctgttcc
     2281 gcaagagcaa cctgaagcct ttcgaaaggg acatcagcac cgaaatctac caggctggat
     2341 ctaccccatg caacggagtg gaaggattca actgctactt ccctctgcag tcttacggat
     2401 tccagccaac caacggagtg ggataccagc catacagagt ggtggtgctg tctttcgagc
     2461 tgctgcacgc tcctgctacc gtgtgcggac caaagaagtc taccaacctg gtgaagaaca
     2521 agtgcgtgaa cttcaacttc aacggactga ccggaaccgg agtgctgacc gaatccaaca
     2581 agaagttcct gcctttccag cagttcggta gagacatcgc cgacaccacc gacgctgtga
     2641 gagacccaca gaccctggaa atcctggaca tcaccccctg ctctttcgga ggagtgagcg
     2701 tgatcacccc aggaaccaac acctccaacc aggtggccgt gctgtaccag gacgtgaact
     2761 gcaccgaagt gccagtggcc atccacgctg accagctgac cccaacctgg agagtgtaca
     2821 gcaccggatc taacgtgttc cagacccgtg ccggctgcct gatcggagcc gagcacgtga
     2881 acaactccta cgaatgcgac atcccaatcg gagctggcat ctgcgcttct taccagaccc
     2941 agaccaacag cccaagaagg gccagatccg tggcctctca gagcatcatc gcctacacca
     3001 tgagcctggg cgctgaaaac agcgtggctt acagcaacaa ctctatcgcc atcccaacca
     3061 acttcaccat ctctgtgacc accgaaatcc tgccagtgag catgaccaag accagcgtgg
     3121 actgcaccat gtacatctgc ggagactcta ccgaatgctc taacctgctg ctgcagtacg
     3181 gcagcttctg cacccagctg aaccgcgctc tgaccggaat cgctgtggag caggacaaga
     3241 acacccagga agtgttcgct caggtgaagc agatatacaa gaccccacct atcaaggact
     3301 tcggaggatt caacttctct cagatcctgc ccgacccatc taagccctct aagaggtctt
     3361 tcatcgaaga cctgctgttc aacaaggtga ccctggccga cgccggtttc atcaagcagt
     3421 acggtgactg cctgggagac atcgccgcta gagacctgat ctgcgcccag aagttcaacg
     3481 gtctgaccgt gctgccacca ctgctgaccg acgagatgat cgcccagtac acctctgccc
     3541 tgctggctgg aaccatcacc tctggttgga ccttcggagc tggcgccgcc ctgcagatcc
     3601 cattcgctat gcagatggcc taccgtttca acggtatcgg agtgacccag aacgtgctgt
     3661 acgaaaacca gaagctgatc gctaaccagt tcaactctgc tatcggaaag atccaggact
     3721 ccctgtcctc taccgcttct gccctgggaa agctgcagga cgtggtgaac cagaacgccc
     3781 aggccctgaa caccctggtg aagcagctgt cttctaactt cggagccatc tcctctgtgc
     3841 tgaacgacat cctggctagg ctggacaagg tggaagccga ggtgcagatc gacagactga
     3901 tcaccggaag actgcagtct ctgcagacct acgtgaccca gcagctgatc agggccgctg
     3961 aaatcagagc ttctgccaac ctggctgcca ccaagatgtc cgaatgcgtg ctgggccagt
     4021 ctaagagggt ggacttctgc ggaaagggat accacctgat gtccttccca cagtctgctc
     4081 cacacggagt ggtgttcctg cacgtgacct acgtgcccgc ccaggaaaag aacttcacca
     4141 ccgccccagc tatctgccac gacggaaagg ctcacttccc cagggaggga gtgttcgtgt
     4201 ctaacggaac ccactggttc gtgacccaga ggaacttcta cgagccacag atcatcacca
     4261 ccgacaacac cttcgtgtct ggaaactgcg acgtggtgat cggaatcgtg aacaacaccg
     4321 tgtacgaccc actgcagcca gaactggact ctttcaagga ggaactggac aagtacttca
     4381 agaaccacac ctctccagac gtggacctgg gagacatctc cggaatcaac gcctctgtgg
     4441 tgaacatcca gaaggaaatc gacagactga acgaggtggc caagaacctg aacgaatctc
     4501 tgatcgacct gcaggaactg ggaaagtacg agcagtacat caagtggccc tggtacatct
     4561 ggctgggttt catcgctggt ctgatcgcta tcgtgatggt gaccatcatg ctgtgctgca
     4621 tgacctcttg ctgctcttgc ctgaagggat gctgctcttg cggatcttgc tgcaagttcg
     4681 acgaggacga ctccgagcct gtgctgaagg gtgtgaagct gcactacacc tacccaactt
     4741 tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg
     4801 tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc tatagtgagt
     4861 cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat
     4921 gttacattgc acaagataaa aatatatcat catgcctcct ctagaccagc caggacagaa
     4981 atgcctcgac ttcgctgcta cccaaggttg ccgggtgacg cacaccgtgg aaacggatga
     5041 aggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta
     5101 tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg
     5161 cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct cgggcatcca
     5221 agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta
     5281 cgcagcaggg cagtcgccct aaaacaaagt taaacattat gagggaagcg gtgatcgccg
     5341 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt
     5401 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata
     5461 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca
     5521 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag
     5581 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc
     5641 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg
     5701 acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc
     5761 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg
     5821 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc
     5881 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg
     5941 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta
     6001 gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag
     6061 aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa ccctcgagcc
     6121 acccatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg
     6181 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6241 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6301 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     6361 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6421 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6481 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6541 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6601 aaagcgcca
//
