LOCUS       sequence-352095-        6609 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sars-CoV-2 protein mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6609)
  AUTHORS   Liu X, Huuskonen S, Laitinen T, Redchuk T, Bogacheva M, Salokas K, 
            Pohner I, Ohman T, Tonduru AK, Hassinen A, Gawriyski L, Keskitalo S,
            Vartiainen MK, Pietiainen V, Poso A, Varjosalo M
  TITLE     SARS-CoV-2-host proteome interactions for antiviral drug discovery.
  JOURNAL   Mol Syst Biol. 2021 Nov;17(11):e10396. doi: 10.15252/msb.202110396.
  PUBMED    34709727
REFERENCE   2  (bases 1 to 6609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2021-11"; volume: "17(11):e10396. doi"; pages: " 
            10.15252/msb.202110396"
COMMENT     Sequence Label: pDON223 Spike H655Y
FEATURES             Location/Qualifiers
     source          1..6609
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(114..141)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(233..319)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     368..390
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     382..399
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     383..399
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    415..514
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4334..4433)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4450..4469)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4451..4469)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4474..4490)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4474..4490)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4921..5712
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5805..6393
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6294..6313
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6547..6564
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
       61 cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc
      121 catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg
      181 cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc
      241 tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact
      301 gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg
      361 atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccccaaata
      421 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt
      481 tttataatgc caactttgta caaaaaagtt ggcatgttcg tgttcctggt gctgctgcca
      541 ctggtgagct cccagtgcgt gaacctgacc accagaaccc agctgccacc tgcctacacc
      601 aactccttca ccaggggcgt gtactaccca gacaaggtgt tcagatccag cgtgctgcac
      661 tctacccagg acctgttcct gccattcttc tctaacgtga cctggttcca cgccatccac
      721 gtgtctggaa ccaacggcac caagagattc gacaacccag tgctgccatt caacgacggc
      781 gtgtacttcg cttctaccga gaagtctaac atcatcaggg gttggatctt cggaaccacc
      841 ctggactcta agacccagtc tctgctgatc gtgaacaacg ctaccaacgt ggtgatcaag
      901 gtgtgcgagt tccagttctg caacgaccca ttcctgggag tgtactacca caagaacaac
      961 aagtcttgga tggaaagcga gttcagggtg tacagctctg ctaacaactg caccttcgag
     1021 tacgtgtctc agcctttcct gatggacctg gaaggaaagc agggaaactt caagaacctg
     1081 agagagttcg tgttcaagaa catcgacggt tacttcaaga tatacagcaa gcacacccca
     1141 atcaacctgg tgagggacct gccacagggt ttctctgctc tggagcccct ggtggacctg
     1201 ccaatcggca tcaacatcac caggttccag accctgctgg ctctgcacag gtcttacctg
     1261 accccaggcg acagcagctc tggatggacc gccggcgccg ctgcttacta cgtgggatac
     1321 ctgcagccaa ggaccttcct gctgaagtac aacgaaaacg gcaccatcac cgacgccgtg
     1381 gactgcgctc tggacccact gtctgagacc aagtgcaccc tgaagtcttt caccgtggaa
     1441 aagggaatct accagaccag caacttccgc gtgcagccca ccgagtctat cgtgagattc
     1501 ccaaacatca ccaacctgtg cccattcgga gaagtgttca acgctaccag gttcgccagc
     1561 gtgtacgctt ggaacaggaa gagaatcagc aactgcgtgg ccgactactc tgtgctgtac
     1621 aactctgcct ccttctccac cttcaagtgc tacggcgtgt ccccaaccaa gctgaacgac
     1681 ctgtgcttca ccaacgtgta cgctgactcc ttcgtgatca gaggagacga agtgagacag
     1741 atcgctcccg gtcagaccgg aaagatcgcc gactacaact acaagctgcc agacgacttc
     1801 accggttgcg tgatcgcttg gaactctaac aacctggact ctaaggtggg aggcaactac
     1861 aactacctgt acaggctgtt ccgcaagagc aacctgaagc ctttcgaaag ggacatcagc
     1921 accgaaatct accaggctgg atctacccca tgcaacggag tggaaggatt caactgctac
     1981 ttccctctgc agtcttacgg attccagcca accaacggag tgggatacca gccatacaga
     2041 gtggtggtgc tgtctttcga gctgctgcac gctcctgcta ccgtgtgcgg accaaagaag
     2101 tctaccaacc tggtgaagaa caagtgcgtg aacttcaact tcaacggact gaccggaacc
     2161 ggagtgctga ccgaatccaa caagaagttc ctgcctttcc agcagttcgg tagagacatc
     2221 gccgacacca ccgacgctgt gagagaccca cagaccctgg aaatcctgga catcaccccc
     2281 tgctctttcg gaggagtgag cgtgatcacc ccaggaacca acacctccaa ccaggtggcc
     2341 gtgctgtacc aggacgtgaa ctgcaccgaa gtgccagtgg ccatccacgc tgaccagctg
     2401 accccaacct ggagagtgta cagcaccgga tctaacgtgt tccagacccg tgccggctgc
     2461 ctgatcggag ccgagtacgt gaacaactcc tacgaatgcg acatcccaat cggagctggc
     2521 atctgcgctt cttaccagac ccagaccaac agcccaagaa gggccagatc cgtggcctct
     2581 cagagcatca tcgcctacac catgagcctg ggcgctgaaa acagcgtggc ttacagcaac
     2641 aactctatcg ccatcccaac caacttcacc atctctgtga ccaccgaaat cctgccagtg
     2701 agcatgacca agaccagcgt ggactgcacc atgtacatct gcggagactc taccgaatgc
     2761 tctaacctgc tgctgcagta cggcagcttc tgcacccagc tgaaccgcgc tctgaccgga
     2821 atcgctgtgg agcaggacaa gaacacccag gaagtgttcg ctcaggtgaa gcagatatac
     2881 aagaccccac ctatcaagga cttcggagga ttcaacttct ctcagatcct gcccgaccca
     2941 tctaagccct ctaagaggtc tttcatcgaa gacctgctgt tcaacaaggt gaccctggcc
     3001 gacgccggtt tcatcaagca gtacggtgac tgcctgggag acatcgccgc tagagacctg
     3061 atctgcgccc agaagttcaa cggtctgacc gtgctgccac cactgctgac cgacgagatg
     3121 atcgcccagt acacctctgc cctgctggct ggaaccatca cctctggttg gaccttcgga
     3181 gctggcgccg ccctgcagat cccattcgct atgcagatgg cctaccgttt caacggtatc
     3241 ggagtgaccc agaacgtgct gtacgaaaac cagaagctga tcgctaacca gttcaactct
     3301 gctatcggaa agatccagga ctccctgtcc tctaccgctt ctgccctggg aaagctgcag
     3361 gacgtggtga accagaacgc ccaggccctg aacaccctgg tgaagcagct gtcttctaac
     3421 ttcggagcca tctcctctgt gctgaacgac atcctgtcta ggctggacaa ggtggaagcc
     3481 gaggtgcaga tcgacagact gatcaccgga agactgcagt ctctgcagac ctacgtgacc
     3541 cagcagctga tcagggccgc tgaaatcaga gcttctgcca acctggctgc caccaagatg
     3601 tccgaatgcg tgctgggcca gtctaagagg gtggacttct gcggaaaggg ataccacctg
     3661 atgtccttcc cacagtctgc tccacacgga gtggtgttcc tgcacgtgac ctacgtgccc
     3721 gcccaggaaa agaacttcac caccgcccca gctatctgcc acgacggaaa ggctcacttc
     3781 cccagggagg gagtgttcgt gtctaacgga acccactggt tcgtgaccca gaggaacttc
     3841 tacgagccac agatcatcac caccgacaac accttcgtgt ctggaaactg cgacgtggtg
     3901 atcggaatcg tgaacaacac cgtgtacgac ccactgcagc cagaactgga ctctttcaag
     3961 gaggaactgg acaagtactt caagaaccac acctctccag acgtggacct gggagacatc
     4021 tccggaatca acgcctctgt ggtgaacatc cagaaggaaa tcgacagact gaacgaggtg
     4081 gccaagaacc tgaacgaatc tctgatcgac ctgcaggaac tgggaaagta cgagcagtac
     4141 atcaagtggc cctggtacat ctggctgggt ttcatcgctg gtctgatcgc tatcgtgatg
     4201 gtgaccatca tgctgtgctg catgacctct tgctgctctt gcctgaaggg atgctgctct
     4261 tgcggatctt gctgcaagtt cgacgaggac gactccgagc ctgtgctgaa gggtgtgaag
     4321 ctgcactaca cctacccaac tttcttgtac aaagttggca ttataagaaa gcattgctta
     4381 tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca
     4441 gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg
     4501 cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgcctc
     4561 ctctagacca gccaggacag aaatgcctcg acttcgctgc tacccaaggt tgccgggtga
     4621 cgcacaccgt ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt
     4681 aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc
     4741 agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta
     4801 cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg
     4861 ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatt
     4921 atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc
     4981 gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc
     5041 ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa
     5101 acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc
     5161 gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt
     5221 tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt
     5281 atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa
     5341 catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag
     5401 gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct
     5461 ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc
     5521 aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat
     5581 cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc
     5641 tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta
     5701 gtcggcaaat aaccctcgag ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg
     5761 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     5821 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     5881 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     5941 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6001 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6061 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6121 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6181 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     6241 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     6301 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     6361 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     6421 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     6481 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     6541 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     6601 cccgcgcgt
//
