LOCUS       sequence-352128-        3441 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sars-CoV-2 protein mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Liu X, Huuskonen S, Laitinen T, Redchuk T, Bogacheva M, Salokas K, 
            Pohner I, Ohman T, Tonduru AK, Hassinen A, Gawriyski L, Keskitalo S,
            Vartiainen MK, Pietiainen V, Poso A, Varjosalo M
  TITLE     SARS-CoV-2-host proteome interactions for antiviral drug discovery.
  JOURNAL   Mol Syst Biol. 2021 Nov;17(11):e10396. doi: 10.15252/msb.202110396.
  PUBMED    34709727
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2021-11"; volume: "17(11):e10396. doi"; pages: " 
            10.15252/msb.202110396"
COMMENT     Sequence Label: pDON223 N S235F
FEATURES             Location/Qualifiers
     source          1..3441
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(69..96)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     155..177
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     169..186
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     170..186
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    202..301
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(490..501)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(1121..1220)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1237..1256)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1238..1256)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1261..1277)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1261..1277)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1708..2499
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2592..3180
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3081..3100
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3334..3351
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta
       61 gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttc
      121 cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg
      181 gccagtctta agctcgggcc ccaaataatg attttatttt gactgatagt gacctgttcg
      241 ttgcaacaaa ttgatgagca atgctttttt ataatgccaa ctttgtacaa aaaagttggc
      301 atgtccgaca acggtcccca gaaccagcgt aacgctccaa ggatcacctt cggtggacct
      361 agcgactcca ccggcagcaa ccagaacgga gaacgctccg gagccagaag caagcagcgt
      421 aggccacagg gcctgcctaa caacaccgcc agctggttca ccgctctgac ccagcacgga
      481 aaggaagacc tgaagttccc aaggggacag ggcgtgccaa tcaacaccaa cagctctcca
      541 gacgaccaga tcggatacta caggcgtgcc accaggcgca tcagaggagg cgacggaaag
      601 atgaaggacc tgtctcctag gtggtacttc tactacctgg gcaccggacc agaggctggt
      661 ctgccatacg gcgccaacaa ggacggcatc atctgggtgg ctaccgaggg agctctgaac
      721 accccaaagg accacatcgg aacccgtaac cctgccaaca acgctgccat cgtgctgcag
      781 ctgccacagg gaaccaccct gcccaaggga ttctacgctg agggctccag gggtggaagc
      841 caggctagct ctcgttcttc cagccgttct aggaacagct cccgcaactc taccccagga
      901 agctccagag gcaccagccc agctcgtatg gccggcaacg gaggtgacgc cgctctggct
      961 ctgctgctgc tggaccgtct gaaccagctg gaaagcaaga tgttcggaaa gggtcagcag
     1021 cagcagggcc agaccgtgac cctgctgcca gctgctgacc tggacgactt ctccaagcag
     1081 ctgcagcagt ctatgagctc tgctgactcc acccaggctt acccaacttt cttgtacaaa
     1141 gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
     1201 caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg
     1261 gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca
     1321 caagataaaa atatatcatc atgcctcctc tagaccagcc aggacagaaa tgcctcgact
     1381 tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc
     1441 cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc
     1501 aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg
     1561 gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg
     1621 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc
     1681 agtcgcccta aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact
     1741 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
     1801 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg
     1861 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
     1921 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
     1981 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
     2041 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
     2101 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
     2161 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
     2221 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
     2281 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
     2341 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
     2401 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac
     2461 tacgtgaaag gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca
     2521 aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc
     2581 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2641 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2701 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     2761 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2821 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     2881 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     2941 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3001 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3061 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3121 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3181 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3241 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3301 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     3361 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     3421 cacgacaggt ttcccgactg g
//
