LOCUS       sequence-352129-        6609 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sars-CoV-2 protein mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6609)
  AUTHORS   Liu X, Huuskonen S, Laitinen T, Redchuk T, Bogacheva M, Salokas K, 
            Pohner I, Ohman T, Tonduru AK, Hassinen A, Gawriyski L, Keskitalo S,
            Vartiainen MK, Pietiainen V, Poso A, Varjosalo M
  TITLE     SARS-CoV-2-host proteome interactions for antiviral drug discovery.
  JOURNAL   Mol Syst Biol. 2021 Nov;17(11):e10396. doi: 10.15252/msb.202110396.
  PUBMED    34709727
REFERENCE   2  (bases 1 to 6609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2021-11"; volume: "17(11):e10396. doi"; pages: " 
            10.15252/msb.202110396"
COMMENT     Sequence Label: pDON223 Spike D138Y
FEATURES             Location/Qualifiers
     source          1..6609
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     114..133
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     367..384
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(543..570)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(662..748)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     797..819
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     811..828
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     812..828
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    844..943
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4763..4862)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4879..4898)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4880..4898)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4903..4919)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4903..4919)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             5350..6141
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(6234..6609,1..213)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
       61 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
      121 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
      181 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
      241 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
      301 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
      361 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
      421 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
      481 ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg
      541 caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg
      601 ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg
      661 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
      721 cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg
      781 ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg
      841 ccccaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgatgag
      901 caatgctttt ttataatgcc aactttgtac aaaaaagttg gcatgttcgt gttcctggtg
      961 ctgctgccac tggtgagctc ccagtgcgtg aacctgacca ccagaaccca gctgccacct
     1021 gcctacacca actccttcac caggggcgtg tactacccag acaaggtgtt cagatccagc
     1081 gtgctgcact ctacccagga cctgttcctg ccattcttct ctaacgtgac ctggttccac
     1141 gccatccacg tgtctggaac caacggcacc aagagattcg acaacccagt gctgccattc
     1201 aacgacggcg tgtacttcgc ttctaccgag aagtctaaca tcatcagggg ttggatcttc
     1261 ggaaccaccc tggactctaa gacccagtct ctgctgatcg tgaacaacgc taccaacgtg
     1321 gtgatcaagg tgtgcgagtt ccagttctgc aactacccat tcctgggagt gtactaccac
     1381 aagaacaaca agtcttggat ggaaagcgag ttcagggtgt acagctctgc taacaactgc
     1441 accttcgagt acgtgtctca gcctttcctg atggacctgg aaggaaagca gggaaacttc
     1501 aagaacctga gagagttcgt gttcaagaac atcgacggtt acttcaagat atacagcaag
     1561 cacaccccaa tcaacctggt gagggacctg ccacagggtt tctctgctct ggagcccctg
     1621 gtggacctgc caatcggcat caacatcacc aggttccaga ccctgctggc tctgcacagg
     1681 tcttacctga ccccaggcga cagcagctct ggatggaccg ccggcgccgc tgcttactac
     1741 gtgggatacc tgcagccaag gaccttcctg ctgaagtaca acgaaaacgg caccatcacc
     1801 gacgccgtgg actgcgctct ggacccactg tctgagacca agtgcaccct gaagtctttc
     1861 accgtggaaa agggaatcta ccagaccagc aacttccgcg tgcagcccac cgagtctatc
     1921 gtgagattcc caaacatcac caacctgtgc ccattcggag aagtgttcaa cgctaccagg
     1981 ttcgccagcg tgtacgcttg gaacaggaag agaatcagca actgcgtggc cgactactct
     2041 gtgctgtaca actctgcctc cttctccacc ttcaagtgct acggcgtgtc cccaaccaag
     2101 ctgaacgacc tgtgcttcac caacgtgtac gctgactcct tcgtgatcag aggagacgaa
     2161 gtgagacaga tcgctcccgg tcagaccgga aagatcgccg actacaacta caagctgcca
     2221 gacgacttca ccggttgcgt gatcgcttgg aactctaaca acctggactc taaggtggga
     2281 ggcaactaca actacctgta caggctgttc cgcaagagca acctgaagcc tttcgaaagg
     2341 gacatcagca ccgaaatcta ccaggctgga tctaccccat gcaacggagt ggaaggattc
     2401 aactgctact tccctctgca gtcttacgga ttccagccaa ccaacggagt gggataccag
     2461 ccatacagag tggtggtgct gtctttcgag ctgctgcacg ctcctgctac cgtgtgcgga
     2521 ccaaagaagt ctaccaacct ggtgaagaac aagtgcgtga acttcaactt caacggactg
     2581 accggaaccg gagtgctgac cgaatccaac aagaagttcc tgcctttcca gcagttcggt
     2641 agagacatcg ccgacaccac cgacgctgtg agagacccac agaccctgga aatcctggac
     2701 atcaccccct gctctttcgg aggagtgagc gtgatcaccc caggaaccaa cacctccaac
     2761 caggtggccg tgctgtacca ggacgtgaac tgcaccgaag tgccagtggc catccacgct
     2821 gaccagctga ccccaacctg gagagtgtac agcaccggat ctaacgtgtt ccagacccgt
     2881 gccggctgcc tgatcggagc cgagcacgtg aacaactcct acgaatgcga catcccaatc
     2941 ggagctggca tctgcgcttc ttaccagacc cagaccaaca gcccaagaag ggccagatcc
     3001 gtggcctctc agagcatcat cgcctacacc atgagcctgg gcgctgaaaa cagcgtggct
     3061 tacagcaaca actctatcgc catcccaacc aacttcacca tctctgtgac caccgaaatc
     3121 ctgccagtga gcatgaccaa gaccagcgtg gactgcacca tgtacatctg cggagactct
     3181 accgaatgct ctaacctgct gctgcagtac ggcagcttct gcacccagct gaaccgcgct
     3241 ctgaccggaa tcgctgtgga gcaggacaag aacacccagg aagtgttcgc tcaggtgaag
     3301 cagatataca agaccccacc tatcaaggac ttcggaggat tcaacttctc tcagatcctg
     3361 cccgacccat ctaagccctc taagaggtct ttcatcgaag acctgctgtt caacaaggtg
     3421 accctggccg acgccggttt catcaagcag tacggtgact gcctgggaga catcgccgct
     3481 agagacctga tctgcgccca gaagttcaac ggtctgaccg tgctgccacc actgctgacc
     3541 gacgagatga tcgcccagta cacctctgcc ctgctggctg gaaccatcac ctctggttgg
     3601 accttcggag ctggcgccgc cctgcagatc ccattcgcta tgcagatggc ctaccgtttc
     3661 aacggtatcg gagtgaccca gaacgtgctg tacgaaaacc agaagctgat cgctaaccag
     3721 ttcaactctg ctatcggaaa gatccaggac tccctgtcct ctaccgcttc tgccctggga
     3781 aagctgcagg acgtggtgaa ccagaacgcc caggccctga acaccctggt gaagcagctg
     3841 tcttctaact tcggagccat ctcctctgtg ctgaacgaca tcctgtctag gctggacaag
     3901 gtggaagccg aggtgcagat cgacagactg atcaccggaa gactgcagtc tctgcagacc
     3961 tacgtgaccc agcagctgat cagggccgct gaaatcagag cttctgccaa cctggctgcc
     4021 accaagatgt ccgaatgcgt gctgggccag tctaagaggg tggacttctg cggaaaggga
     4081 taccacctga tgtccttccc acagtctgct ccacacggag tggtgttcct gcacgtgacc
     4141 tacgtgcccg cccaggaaaa gaacttcacc accgccccag ctatctgcca cgacggaaag
     4201 gctcacttcc ccagggaggg agtgttcgtg tctaacggaa cccactggtt cgtgacccag
     4261 aggaacttct acgagccaca gatcatcacc accgacaaca ccttcgtgtc tggaaactgc
     4321 gacgtggtga tcggaatcgt gaacaacacc gtgtacgacc cactgcagcc agaactggac
     4381 tctttcaagg aggaactgga caagtacttc aagaaccaca cctctccaga cgtggacctg
     4441 ggagacatct ccggaatcaa cgcctctgtg gtgaacatcc agaaggaaat cgacagactg
     4501 aacgaggtgg ccaagaacct gaacgaatct ctgatcgacc tgcaggaact gggaaagtac
     4561 gagcagtaca tcaagtggcc ctggtacatc tggctgggtt tcatcgctgg tctgatcgct
     4621 atcgtgatgg tgaccatcat gctgtgctgc atgacctctt gctgctcttg cctgaaggga
     4681 tgctgctctt gcggatcttg ctgcaagttc gacgaggacg actccgagcc tgtgctgaag
     4741 ggtgtgaagc tgcactacac ctacccaact ttcttgtaca aagttggcat tataagaaag
     4801 cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt
     4861 tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg
     4921 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca
     4981 tcatgcctcc tctagaccag ccaggacaga aatgcctcga cttcgctgct acccaaggtt
     5041 gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt
     5101 tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg
     5161 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt
     5221 tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga
     5281 tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag
     5341 ttaaacatta tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt
     5401 ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca
     5461 gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg
     5521 cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct
     5581 ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt
     5641 ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt
     5701 cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa
     5761 gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt
     5821 cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc
     5881 gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca
     5941 gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg
     6001 gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat
     6061 cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc
     6121 accaaggtag tcggcaaata accctcgagc cacccatgac caaaatccct taacgtgagt
     6181 tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     6241 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     6301 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     6361 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     6421 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     6481 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     6541 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     6601 gaactgaga
//
