LOCUS       sequence-35213-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  TITLE     LIC N-terminal fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET His6 MBP Asn10 TEV LIC cloning vector (1C)
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(6..23)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(89..109)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(167..190)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
ORIGIN
        1 tctcagtggt ggtggtggtg gtgctcgagt gcggccgcaa gcttgtcgac ggcgctcgaa
       61 ttcggatccg ttatccactt ccaatattgg attggaagta caggttttcc tcgatcccga
      121 ggttgttgtt attgttattg ttgttgttgt tcgagctcga attagtctgc gcgtctttca
      181 gggcttcatc gacagtctga cgaccgctgg cggcgttgat caccgcagta cgcacggcat
      241 accagaaagc ggacatctgc gggatgttcg gcatgatttc acctttctgg gcgttttcca
      301 tagtggcggc aatacgtgga tctttcgcca actcttcctc gtaagacttc agcgctacgg
      361 cacccagcgg tttgtcttta ttaaccgctt ccagaccttc atcagtcagc agatagtttt
      421 cgaggaactc ttttgccagc tctttgttcg gactggcggc gttaatacct gcgctcagca
      481 cgccaacgaa cggtttggat ggttgaccct tgaaggtcgg cagtaccgtt acaccataat
      541 tcactttgct ggtgtcgatg ttggaccatg cccacgggcc gttgatggtc atcgctgttt
      601 cgcctttatt aaaggcagct tctgcgatgg agtaatcggt gtctgcattc atgtgtttgt
      661 ttttaatcag gtcaaccagg aaggtcagac ccgctttcgc gccagcgtta tccacgccca
      721 cgtctttaat gtcgtacttg ccgttttcat acttgaacgc ataacccccg tcagcagcaa
      781 tcagcggcca ggtgaagtac ggttcttgca ggttgaacat cagcgcgctc ttaccttt
//
