LOCUS       sequence-352134-        3375 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sars-CoV-2 protein mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Liu X, Huuskonen S, Laitinen T, Redchuk T, Bogacheva M, Salokas K, 
            Pohner I, Ohman T, Tonduru AK, Hassinen A, Gawriyski L, Keskitalo S,
            Vartiainen MK, Pietiainen V, Poso A, Varjosalo M
  TITLE     SARS-CoV-2-host proteome interactions for antiviral drug discovery.
  JOURNAL   Mol Syst Biol. 2021 Nov;17(11):e10396. doi: 10.15252/msb.202110396.
  PUBMED    34709727
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2021-11"; volume: "17(11):e10396. doi"; pages: " 
            10.15252/msb.202110396"
COMMENT     Sequence Label: pDON221 ORF3a Q57H
FEATURES             Location/Qualifiers
     source          1..3375
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     106..125
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     359..376
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(535..562)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(654..740)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     789..811
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     803..820
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     804..820
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    836..935
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1767..1866)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1883..1902)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1884..1902)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1907..1923)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1907..1923)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2025..2044)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2036..2845
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2103..2122)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      join(2992..3375,1..205)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
       61 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
      121 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
      181 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
      241 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
      301 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
      361 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
      421 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
      481 gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg
      541 ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct
      601 gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc
      661 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac
      721 tgagcctttc gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg
      781 gatgttttcc cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccccaaat
      841 aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgatg agcaatgctt
      901 ttttataatg ccaactttgt acaaaaaagc aggctccacc atggaccttt ttatgagaat
      961 ctttaccatc gggactgtca cactgaaaca gggagaaatt aaggacgcga ccccgagtga
     1021 ctttgtgagg gccactgcta caattcccat ccaagcctct cttccattcg gctggctcat
     1081 agtgggtgtg gcactgctcg cggtattcca ctcagcgtct aagatcataa cgctgaagaa
     1141 gagatggcag ttggctctca gcaaaggtgt tcatttcgtc tgtaacctgc ttcttctttt
     1201 cgtcactgtc tattctcact tgctcttggt tgcagccggg ctggaagccc cgtttctgta
     1261 tctgtacgcg ctcgtttact tcctccagag cataaatttc gtacgaatta ttatgagact
     1321 ctggttgtgc tggaagtgtc ggagcaagaa tcctctcttg tatgacgcca attacttcct
     1381 ttgttggcac actaattgtt atgattactg cataccttac aatagcgtta cctcatccat
     1441 agtaatcacc tccggtgacg gaacaacgtc accaatctcc gagcatgatt accagattgg
     1501 aggctatacg gaaaaatggg agagtggtgt caaggactgt gtggtgctgc atagctattt
     1561 cacaagcgat tattatcaac tgtacagtac acagttgtcc acggataccg gggtcgagca
     1621 tgtcacattt tttatttata acaagatcgt tgatgagcca gaggagcatg tgcagatcca
     1681 cacgatagat ggaagctccg gtgtagtgaa tccagtcatg gaaccgatct atgacgagcc
     1741 caccacgacc acatctgtgc ccctggaccc agctttcttg tacaaagttg gcattataag
     1801 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
     1861 tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc
     1921 ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
     1981 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag
     2041 ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata
     2101 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta
     2161 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga
     2221 tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat
     2281 caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa
     2341 aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct
     2401 ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga
     2461 tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag
     2521 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa
     2581 acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct
     2641 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga
     2701 ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca
     2761 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca
     2821 tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag
     2881 cattacgctg acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta
     2941 cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3001 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3061 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     3121 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     3181 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     3241 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     3301 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     3361 actgagatac ctaca
//
