LOCUS       sequence-35214-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  TITLE     LIC N-terminal fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET His6 GST TEV LIC cloning vector (1G)
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..38)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(104..124)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(134..787)
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(154..176)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             complement(797..814)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc cgcaagcttg
       61 tcgacggcgc tcgaattcgg atccgttatc cacttccaat attggattgg aagtacaggt
      121 tttcctcgat cccttttgga ggatggtcgc caccaccaaa cgtggcttgc cagccctgca
      181 aaggccatgc tatatacttg ctggatttca agtacttatc aatttgtggg atagcttcaa
      241 tacgtttttt aaaacaaact aattttggga acgcatccag gcacattggg tccatgtata
      301 aaacaacatc aagagcgtca tacaacatga agtcaggatg ggttacatga tcaccattta
      361 aatatgtttt atgacataaa cgatcttcga acattttcag catttcaggt agcttgctaa
      421 gaaaatcaac tttgagagtt tcaaagtctt tactatatgc aattctcgaa acaccgtatc
      481 taatatccaa aaccgctcct tcaagcattg aaatctctgc acgctctttt ggacaaccac
      541 ccaacatgtt gtgcttgtca gctatataac gtatgatggc catagactgt gttaatttaa
      601 catcaccatc aatataataa ggaagattgg gaaactccaa acccaattca aactttttgt
      661 ttcgccattt atcaccttca tcgcgctcat acaaatgctc ttcatatttt tcttcaagat
      721 attccaaaag aagtcgagtg ggttgcacaa ggcccttaat tttccaataa cctagtatag
      781 gggacataga agaaccatgg tgatggtgat ggtgagaaga acccatggta tatctccttc
      841 taagtaacaa
//
