LOCUS       sequence-352159-        3717 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC30A3_STOP
FEATURES             Location/Qualifiers
     source          1..3717
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1274..1373)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1390..1409)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1391..1409)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1414..1430)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1414..1430)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1532..1551)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1543..2352
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1610..1629)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2499..3087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2988..3007
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3241..3258
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3417..3444)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3536..3622)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3671..3693
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3685..3702
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3686..3702
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgga gccttcccca
      121 gcagcaggag gactggagac cacaaggctg gtgtctccaa gggacagggg aggagcagga
      181 ggatccctgc gcctgaagtc tctgtttaca gagccctctg agcccctgcc tgaggagagc
      241 aagcctgtgg agatgccatt ccaccactgc cacagggatc cactgccacc tccaggactg
      301 acccctgaga gactgcacgc acggagacag ctgtacgcag catgcgccgt gtgcttcgtg
      361 tttatggcag gagaggtggt gggaggatat ctggcacact ccctggccat catgacagac
      421 gcagcacacc tgctggcaga tgtgggatct atgatgggct ccctgttttc tctgtggctg
      481 agcaccaggc cagcaaccag aacaatgacc ttcggctggc acaggtctga gacactgggc
      541 gccctggcaa gcgtggtgtc cctgtggatg gtgaccggca tcctgctgta cctggccttt
      601 gtgcgcctgc tgcacagcga ctatcacatc gagggaggag caatgctgct gacagcatcc
      661 atcgccgtgt gcgccaacct gctgatggcc ttcgtgctgc accaggcagg accacctcac
      721 tctcacggaa gcaggggagc agagtacgca ccactggagg agggccctga ggagccactg
      781 cccctgggca ataccagcgt gagagcagcc ttcgtgcacg tgctgggcga cctgctgcag
      841 tcctttggcg tgctggccgc ctctatcctg atctacttca agccacagta taaggccgcc
      901 gatcccatca gcacattcct gttttccatc tgtgcactgg gatctacagc acccaccctg
      961 agggacgtgc tgaggatcct gatggagggc acccctagga acgtgggctt tgagccagtg
     1021 cgcgatacac tgctgagcgt gcctggcgtg cgggcaaccc acgagctgca cctgtgggcc
     1081 ctgacactga cctatcacgt ggcaagcgcc cacctggcaa tcgactccac agcagatcct
     1141 gaggccgtgc tggcagaggc aagctccagg ctgtactcca gattcggctt ttctagctgt
     1201 accctgcagg tggagcagta tcagccagag atggcacagt gcctgaggtg tcaggagcca
     1261 ccccaggcct aagacccagc tttcttgtac aaagttggca ttataagaaa gcattgctta
     1321 tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca
     1381 gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg
     1441 cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca
     1501 ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg
     1561 gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg
     1621 gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat
     1681 gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag
     1741 atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc
     1801 cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag
     1861 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg
     1921 cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt
     1981 ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac
     2041 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc
     2101 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag
     2161 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat
     2221 cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt
     2281 caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat
     2341 gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact
     2401 tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac
     2461 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     2521 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     2581 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     2641 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     2701 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     2761 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     2821 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     2881 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     2941 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     3001 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     3061 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     3121 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     3181 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     3241 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     3301 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     3361 gagcgcaacg caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa
     3421 ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc
     3481 ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg
     3541 tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg
     3601 actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca
     3661 tggatgtttt cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggccc
//
