LOCUS       sequence-35216-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  TITLE     LIC N-terminal fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET His6 NusA TEV LIC cloning vector (1N)
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(112..132)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg
       61 caagcttgtc gacggcgctc gaattcggat ccgttatcca cttccaatat tggattggaa
      121 gtacaggttt tcctcgatcc ccgcttcgtc accgaaccag caaatgttac gggcagccat
      181 aatcagtgct ccggcttttt cgtcggtcaa cccttcgata tcagccagat catcaatgcc
      241 ctgttcggcg agatcttcca gcgtacaaac gccacgggcg gccagtttga atgccaaatc
      301 acgatctacc ccttcaaggt tcagcagatc gtcagccggt ttgttatcac cgaggctttc
      361 ttcctgggcc tgtgcaatgg tggccagtgc atttttagca cgctcgcgca gtgcttcaac
      421 ggtcggctca tcaaggcctt cgatttccaa cagctctttc atcggcacat aggccaattc
      481 ttccagcgtc gagaagcctt cttctaccag aacagtcgcg aagtcttcgt cgatgtcgag
      541 atatttggtg aaggtgtcga tcgctgcgtg cgcttccgcc tgatgcttag cctgcaggtc
      601 gtcaacggtc atcacgttga gttcccaacc gctcagctgc gaagccagac gcacgttctg
      661 accgttacgg ccaatcgcct gcgccaggtt accggcttca acggcgatat ccatggtgtg
      721 tttatcttca tccaccacga tagaagcaac gtctgccggt gccattgcgt taatcacgaa
      781 ctgcgccggg ttatcatccc acaggacgat atcgatacgc tcgccaccca gttcagtaga
      841 caccgcctga acacgcgcgc cacgcatacc tacgcaagca cctaccggat cgatacg
//
