LOCUS       sequence-352161-        4689 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4689)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLCO1C1_STOP
FEATURES             Location/Qualifiers
     source          1..4689
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2246..2345)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2362..2381)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2363..2381)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2386..2402)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2386..2402)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2504..2523)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2515..3324
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2582..2601)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3471..4059
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3960..3979
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4213..4230
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4389..4416)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4508..4594)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4643..4665
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4657..4674
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4658..4674
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgga taccagctcc
      121 aaggagaaca tccagctgtt ctgcaagaca agcgtgcagc cagtgggccg cccttccttt
      181 aagaccgagt acccctctag cgaggagaag cagccttgct gtggcgagct gaaggtgttc
      241 ctgtgcgccc tgagcttcgt gtactttgcc aaggccctgg ccgagggcta tctgaagtcc
      301 accatcacac agatcgagcg gagatttgac atcccatcct ctctggtggg cgtgatcgat
      361 ggctctttcg agatcggcaa tctgctggtc atcaccttcg tgagctattt tggcgccaag
      421 ctgcacaggc caaagatcat cggagcagga tgcgtgatca tgggagtggg cacactgctg
      481 atcgccatgc ctcagttctt tatggagcag tacaagtatg agcggtacag ccctagctcc
      541 aactccaccc tgagcatctc cccatgcctg ctggagtcta gctcccagct gcccgtgtct
      601 gtgatggaga agtctaagag caagatcagc aatgagtgtg aggtggatac atctagctcc
      661 atgtggatct acgtgttcct gggcaacctg ctgagaggca tcggcgagac cccaatccag
      721 cccctgggca tcgcctatct ggacgatttc gccagcgagg acaatgccgc cttttacatc
      781 ggctgcgtgc agaccgtggc catcatcggc cccatcttcg gctttctgct gggctccctg
      841 tgcgccaagc tgtacgtgga catcggcttt gtgaacctgg atcacatcac catcacacct
      901 aaggacccac agtgggtggg agcatggtgg ctgggctatc tgatcgcagg aatcatctcc
      961 ctgctggcag cagtgccctt ttggtacctg ccaaagtctc tgccccggtc ccagtctaga
     1021 gaggactcca attctagctc cgagaagtct aagttcatca tcgacgatca caccgattat
     1081 cagacacctc agggcgagaa cgccaagatc atggagatgg ccagggactt tctgccaagc
     1141 ctgaagaacc tgttcggcaa tcccgtgtac tttctgtatc tgtgcacctc tacagtgcag
     1201 ttcaatagcc tgtttggcat ggtgacctac aagccaaagt atatcgagca gcagtacggc
     1261 cagtctagct cccgcgccaa tttcgtgatc ggcctgatca acatcccagc agtggccctg
     1321 ggaatcttct ccggcggcat cgtgatgaag aagtttcgga tcagcgtgtg cggagcagca
     1381 aagctgtacc tgggatctag cgtgttcggc tatctgctgt tcctgtccct gtttgccctg
     1441 ggctgtgaga actctgatgt ggcaggactg accgtgagct accagggaac aaagcccgtg
     1501 agctatcacg agagagccct gtttagcgac tgcaattcca ggtgcaagtg tagcgagaca
     1561 aagtgggagc caatgtgcgg cgagaacggc atcacctacg tgagcgcctg cctggcagga
     1621 tgtcagacat ctaacagaag cggcaagaat atcatcttct acaactgcac ctgcgtggga
     1681 atcgcagcaa gcaagtccgg caattcctct ggcatcgtgg gcaggtgcca gaaggataac
     1741 ggctgtcctc agatgttcct gtacttcctg gtcatctctg tgatcaccag ctatacactg
     1801 tccctgggcg gcatccctgg ctacatcctg ctgctgagat gcatcaagcc acagctgaag
     1861 agcttcgccc tgggcatcta tacactggcc atcagggtgc tggcaggaat cccagcaccc
     1921 gtgtacttcg gcgtgctgat cgataccagc tgtctgaagt ggggctttaa gaggtgcgga
     1981 agcaggggat cttgtaggct gtacgactcc aacgtgttcc ggcacatcta tctgggcctg
     2041 accgtgatcc tgggcacagt gtctatcctg ctgagcatcg ccgtgctgtt catcctgaag
     2101 aagaactacg tgtccaagca ccgctctttt atcacaaaga gggagcgcac catggtgagc
     2161 acacggttcc agaaggagaa ttataccaca tccgaccacc tgctgcagcc taactactgg
     2221 ccaggcaagg agacccagct gtaagaccca gctttcttgt acaaagttgg cattataaga
     2281 aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt
     2341 atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc
     2401 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
     2461 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc
     2521 catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat
     2581 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat
     2641 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat
     2701 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc
     2761 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa
     2821 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg
     2881 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat
     2941 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt
     3001 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa
     3061 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt
     3121 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac
     3181 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag
     3241 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat
     3301 ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc
     3361 attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac
     3421 gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     3481 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     3541 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     3601 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     3661 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     3721 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3781 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     3841 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     3901 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     3961 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     4021 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     4081 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     4141 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     4201 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     4261 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     4321 aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta
     4381 gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt
     4441 atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc
     4501 ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg
     4561 cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg
     4621 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaag
     4681 ctcgggccc
//
