LOCUS       sequence-352163-        6889 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6889)
  TITLE     pBS-p85alpha
REFERENCE   2  (bases 1 to 6889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Myr-(iSH2-p85)-p110alpha-Myc
FEATURES             Location/Qualifiers
     source          1..6889
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..330,331..345,346..614)
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      181..316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             346..614
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     5'UTR           627..666
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             687..728
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             4239..4268
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    4276..4410
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4300..4319)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4354..4373
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4553..4575)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4716..4735
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4834..4851)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5005..5593)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5085..5104)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5764..6555,6556..6624))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6387..6406
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6625..6729)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6797..6815
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(join(6885..6889,1..16))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
       61 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
      121 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
      181 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
      241 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
      301 ggcttttttg gaggcctagg cttttgcaaa aagctcctcg atcgaggggc tcgcatctct
      361 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
      421 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
      481 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
      541 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
      601 cgccgttaca gatccaagct cctcgagatc ttggtggcgt gaaactcccg cacctcttcg
      661 gccagcgcct tgtagaagcg cgtaccatgg ggagcagcaa gagcaagccc aaggacccca
      721 gccagcgcgg gggacatatg ttatatgagg agtacacccg tacttcccag gaaatccaaa
      781 tgaaaagaac ggctatcgaa gcatttaatg aaaccataaa aatatttgaa gaacaatgcc
      841 aaacccagga gcggtacagc aaagaataca tagggaagtt taaacgcgaa ggcaacgaga
      901 aagaaattca aaggattatg cataaccatg ataagctgaa gtcgcgtatc agtgagatca
      961 ttgacagtag gaggaggttg gaagaagact tgaagaagca ggcagctgag taccgagaga
     1021 tcgacaaacg catgaacagt attaagcccg ggggaatttc cggtggtggt ggtggaatta
     1081 tggtactagt ggaatgttta ctccccaatg gaatgatagt gactttagaa tgcctccgtg
     1141 aggccacact cgtcaccatc aaacatgaac tgttcagaga ggccaggaaa taccctctcc
     1201 atcagcttct gcaagacgaa acttcttaca ttttcgtaag tgtcacccaa gaagcagaaa
     1261 gggaagaatt ttttgatgaa acaagacgac tttgtgacct tcggcttttt caaccctttt
     1321 taaaagttat tgaaccagta ggcaaccgtg aagaaaagat cctcaatcga gaaattggtt
     1381 ttgttattgg catgccagtg tgtgaatttg atatggttaa agatccagaa gtccaagact
     1441 ttcgaaggaa cattctgaat gtttgcaaag aagctgtgga cctgcgggat ctcaactcgc
     1501 ctcatagcag agcaatgtat gtctaccctc caaatgtcga gtcttcccca gaactgccaa
     1561 agcacatcta caacaagtta gataaaggac aaatcatagt ggtgatttgg gtaatagtct
     1621 ctccaaacaa cgacaagcag aagtacactc tgaagatcaa tcatgactgt gtgccagagc
     1681 aagtcattgc tgaagccatc aggaaaaaga ctcggagcat gttgttgtcc tctgagcagc
     1741 tgaaactctg tgtcttagaa tatcagggca agtatattct gaaagtgtgt ggctgtgacg
     1801 aatacttcct ggaaaagtac cctctgagtc agtacaagta cataagaagc tgtataatgc
     1861 tggggaggat gcccaacttg atgctgatgg ccaaagaaag cctatactct cagctgccga
     1921 ttgatagctt caccatgccg tcatactcca ggcgcatctc cacagccaca ccctacatga
     1981 atggagagac atctacgaaa tccctctggg tcataaatag tgcgctcaga ataaaaattc
     2041 tttgtgcaac ctatgtaaat gtaaatattc gagacattga taagatctat gttcgaacag
     2101 gtatctacca tggaggagaa cccttatgtg acaatgtgaa cactcaaaga gtaccttgtt
     2161 ccaatcctag gtggaatgaa tggctgaatt atgatatata cattcctgat cttcctcgtg
     2221 ctgcgcgcct ttgcctttca atctgctctg ttaaaggccg aaagggtgct aaggaggagc
     2281 actgtccgtt ggcctgggga aacataaact tgtttgatta tacagacacc ctagtgtccg
     2341 ggaaaatggc tttgaatctc tggcctgtac cgcatgggtt agaagatctg ctgaacccta
     2401 ttggtgttac tgggtcaaat ccaaataaag aaactccatg cttagagttg gagtttgatt
     2461 ggttcagcag tgtggtgaag tttccagaca tgtctgtgat cgaagaacat gccaattggt
     2521 ccgtgtcccg agaagctgga ttcagttact cccatacagg actgagtaac agactagcca
     2581 gagacaatga gttaagagaa aatgacaagg aacagctccg agcactttgc acccgggacc
     2641 cactatctga aatcactgaa caagagaaag acttcctatg gagccacaga cactactgcg
     2701 taactattcc tgaaatccta cccaaattgc ttctgtctgt caagtggaat tccagagacg
     2761 aagtggccca gatgtactgc ttagtaaaag attggcctcc aatcaaacca gagcaagcca
     2821 tggaactcct ggactgtaac tatccagatc ctatggttcg gagttttgct gttcggtgct
     2881 tagaaaaata tttaacagat gacaaacttt ctcagtacct cattcaactt gtacaggtct
     2941 taaaatatga acagtatttg gataacctgc ttgtgagatt tttactcaag aaagcattga
     3001 caaatcaaag gattggccat tttttctttt ggcatttaaa atctgagatg cacaataaga
     3061 ctgtcagtca gaggtttggc ctgctattgg agtcctactg ccgtgcctgt gggatgtatc
     3121 tgaagcacct gaacagacaa gtagaggcca tggagaagct catcaaccta acggacatcc
     3181 ttaagcagga gaagaaggat gagacacaaa aggtacagat gaagtttttg gttgaacaga
     3241 tgagacagcc agacttcatg gatgctttgc agggttttct gtcccctctg aatcctgctc
     3301 accaactagg aaacctcagg cttgaagagt gtcgaattat gtcctctgca aaaaggccac
     3361 tgtggttgaa ttgggagaac ccagacatca tgtcagagct actgtttcag aacaatgaga
     3421 tcatctttaa aaatggcgac gacttacggc aagatatgtt aacccttcag atcatccgaa
     3481 tcatggagaa catctggcaa aaccaaggcc ttgaccttcg catgctacct tatggctgtc
     3541 tatccattgg ggactgtgtg ggtctcatcg aggtggtgag aaactctcac accatcatgc
     3601 aaatccagtg caaaggaggc ctgaaggggg cgctgcagtt caacagccac acactgcatc
     3661 aatggctcaa ggacaagaac aagggcgaga tatatgatgc agccattgac ctgttcactc
     3721 ggtcctgcgc tgggtactgc gtggcaacct ttatcttggg aattggagac cggcacaaca
     3781 gcaacatcat ggtgaaagat gacggacagc tgtttcatat agattttggg cactttttgg
     3841 atcacaagaa gaaaaaattt ggctataagc gggaacgtgt gccatttgtg ttgacacagg
     3901 atttcttgat tgtgattagt aagggagcac aagagtacac caagaccaga gagtttgaga
     3961 ggtttcagga gatgtgttac aaggcttacc tagcaattcg gcagcatgcc aatctcttca
     4021 tcaacctttt ttcaatgatg cttggctctg gaatgccaga actacaatct tttgatgaca
     4081 ttgcatatat ccgaaagact ctagccttgg acaaaactga gcaagaagct ttggaatatt
     4141 tcacaaagca aatgaatgat gcacatcatg gtggatggac gacaaaaatg gattggatct
     4201 tccacaccat caagcagcat gctttgaacg agctcgggga gcagaagctg atttccgagg
     4261 aggacctgaa ctgaggatcc agacatgata agatacattg atgagtttgg acaaaccaca
     4321 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     4381 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     4441 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     4501 atggctgatt atgatccggc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac
     4561 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     4621 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac
     4681 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag
     4741 agtgcaccat aggtcgaccg gtcgacctat gcggtgtgaa ataccgcaca gatgcgtaag
     4801 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     4861 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     4921 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     4981 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     5041 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     5101 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     5161 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     5221 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     5281 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     5341 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     5401 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     5461 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     5521 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     5581 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     5641 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     5701 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     5761 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     5821 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     5881 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     5941 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     6001 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     6061 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     6121 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     6181 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     6241 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     6301 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     6361 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     6421 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     6481 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     6541 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     6601 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     6661 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     6721 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     6781 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aattggtcga
     6841 ccaattctca tgtttgacag cttatcatcg ataagcttgg ctgtggaat
//
