LOCUS       sequence-352164-        7111 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sars-CoV-2 protein mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7111)
  AUTHORS   Liu X, Huuskonen S, Laitinen T, Redchuk T, Bogacheva M, Salokas K, 
            Pohner I, Ohman T, Tonduru AK, Hassinen A, Gawriyski L, Keskitalo S,
            Vartiainen MK, Pietiainen V, Poso A, Varjosalo M
  TITLE     SARS-CoV-2-host proteome interactions for antiviral drug discovery.
  JOURNAL   Mol Syst Biol. 2021 Nov;17(11):e10396. doi: 10.15252/msb.202110396.
  PUBMED    34709727
REFERENCE   2  (bases 1 to 7111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2021-11"; volume: "17(11):e10396. doi"; pages: " 
            10.15252/msb.202110396"
COMMENT     Sequence Label: pDON207 NSP3 A890D
FEATURES             Location/Qualifiers
     source          1..7111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(102..188)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    241..340
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4082..4181)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4293..4312)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(4371..4390)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(4964..5497)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5686..5691,5692..5708,5709..5714))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      6371..6959
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6860..6879
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(join(7094..7111,1..10))
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct
       61 ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga
      121 gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt
      181 tttatttgat gcctggcagt tccctactct cgcgttaacg ctagcatgga tctcgggccc
      241 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
      301 tgctttttta taatgccaac tttgtacaaa aaagttggca tggcccctac caaggtgacc
      361 ttcggagacg acaccgtgat cgaggtgcag ggttacaaga gcgtgaacat caccttcgag
      421 ctggacgaac gcatcgacaa ggtgctgaac gaaaagtgct ccgcttacac cgtggagctg
      481 ggcaccgagg tgaacgaatt cgcctgcgtg gtggccgacg ctgtgatcaa gaccctgcag
      541 ccagtgtccg aactgctgac ccctctgggt atcgacctgg acgagtggtc tatggctacc
      601 tactacctgt tcgacgagag cggcgaattc aagctggcct ctcacatgta ctgcagcttc
      661 taccctcccg acgaagacga ggaagagggt gactgcgaag aggaagagtt cgagccttcc
      721 actcagtacg agtacggcac cgaagacgac taccagggca agcctctgga attcggagcc
      781 acctctgccg ctctgcagcc tgaagaggaa caggaggaag actggctgga cgacgactcc
      841 cagcagaccg tgggtcagca ggacggctct gaagacaacc agaccaccac catccagacc
      901 atcgtggagg tgcagcctca gctggagatg gaactgaccc cagtggtgca gaccatcgag
      961 gtgaacagct tctccggcta cctgaagctg accgacaacg tgtacatcaa gaacgctgac
     1021 atcgtggagg aagccaagaa ggtgaaacca accgtggtgg tgaacgccgc taacgtgtac
     1081 ctgaagcacg gcggaggtgt ggctggagct ctgaacaagg ctaccaacaa cgccatgcag
     1141 gtggaatccg acgactacat cgctaccaac ggtcctctga aggtgggcgg atcctgcgtg
     1201 ctgtctggcc acaacctggc caagcactgc ctgcacgtgg tgggaccaaa cgtgaacaag
     1261 ggagaggaca tccagctgct gaagagcgcc tacgaaaact tcaaccagca cgaggtgctg
     1321 ctggctccac tgctgtctgc cggaatcttc ggtgctgatc ccatccacag cctgcgcgtg
     1381 tgcgtggaca ccgtgaggac caacgtgtac ctggccgtgt tcgacaagaa cctgtacgac
     1441 aagctggtga gctccttcct ggagatgaag tccgaaaagc aggtggagca gaagatcgcc
     1501 gagatcccca aggaggaagt gaagcctttc atcaccgaat ctaagcccag cgtggagcag
     1561 agaaagcagg acgacaagaa gatcaaggcc tgcgtggagg aagtgaccac caccctggag
     1621 gaaaccaagt tcctgaccga gaacctgctg ctgtacatcg acatcaacgg caacctgcac
     1681 cctgactccg ctaccctggt gtctgacatc gacatcacct tcctgaagaa ggacgccccc
     1741 tacatcgtgg gcgacgtggt gcaggaagga gtgctgaccg ctgtggtcat ccctaccaag
     1801 aaggccggtg gcaccaccga gatgctggcc aaggctctgc gtaaggtgcc aaccgacaac
     1861 tacatcacca cctaccccgg tcagggcctg aacggttaca ccgtggagga agctaagacc
     1921 gtgctgaaga agtgcaagtc tgccttctac atcctgccct ccatcatctc taacgagaag
     1981 caggaaatcc tgggcaccgt gagctggaac ctgcgcgaaa tgctggccca cgctgaggaa
     2041 accaggaagc tgatgcccgt gtgcgtggag accaaggcca tcgtgagcac catccagaga
     2101 aagtacaagg gcatcaagat ccaggagggt gtggtggact acggcgctcg tttctacttc
     2161 tacacctcca agaccaccgt ggcctctctg atcaacaccc tgaacgacct gaacgagacc
     2221 ctggtgacca tgccactggg atacgtgacc cacggtctga acctggagga agccgctaga
     2281 tacatgcgtt ccctgaaggt gcctgctacc gtgagcgtga gcagccctga cgctgtgacc
     2341 gcctacaacg gatacctgac ctcctctagc aagacccctg aggaacactt catcgaaacc
     2401 atcagcctgg ccggttccta caaggactgg tcttacagcg gacagtccac ccagctgggc
     2461 atcgagttcc tgaagcgcgg agacaagagc gtgtactaca cctccaaccc aaccaccttc
     2521 cacctggacg gtgaagtgat caccttcgac aacctgaaga ccctgctgtc tctgcgcgag
     2581 gtgaggacca tcaaggtgtt caccaccgtg gacaacatca acctgcacac ccaggtggtg
     2641 gacatgagca tgacctacgg acagcagttc ggtcctacct acctggacgg agctgacgtg
     2701 accaagatca agccacacaa ctcccacgag ggcaagacct tctacgtgct gcctaacgat
     2761 gacaccctga gagtggaggc tttcgaatac taccacacca ccgacccatc cttcctgggc
     2821 aggtacatgt ctgccctgaa ccacaccaag aagtggaagt acccccaggt gaacggactg
     2881 accagcatca agtgggccga caacaactgc tacctggcca ccgctctgct gaccctgcag
     2941 cagatcgaac tgaagttcaa cccacccgct ctgcaggacg cttactacag agcccgtgct
     3001 ggcgaggccg ataacttctg cgctctgatc ctggcctact gcaacaagac cgtgggcgaa
     3061 ctgggagacg tgagagagac catgagctac ctgttccagc acgccaacgg cggcaaggga
     3121 ttctgcaagc tgcacaactg gaactgcgtg aactgcgaca ccttctgcgc tggctccacc
     3181 ttcatctctg acgaggtggc cagagacctg agcctgcagt tcaagcgtcc aatcaacccc
     3241 accgaccaga gctcctacat cgtggactct gtgaccgtga agaacggtag catccacctg
     3301 tacttcgaca aggctggcca gaagacctac gagcgccact ctctgagcca cttcgtgaac
     3361 ctggacaacc tgagggccaa caacaccaag ggcagcctgc ctatcaacgt gatcgtgttc
     3421 gacggaaagt ccaagtgcga ggaatctagc gccaagtccg ccagcgtgta ctacagccag
     3481 ctgatgtgcc agccaatcct gctgctggac caggctctgg tgtctgacgt gggagacagc
     3541 gccgaagtgg ccgtgaagat gttcgacgcc tacgtgaaca ccttctcctc taccttcaac
     3601 gtgcctatgg agaagctgaa gaccctggtg gctactgctg aggctgaact ggctaagaac
     3661 gtgtccctgg acaacgtgct gagcaccttc atctccgccg ctaggcaggg tttcgtggac
     3721 tctgacgtgg aaaccaaaga cgtggtggag tgcctgaagc tgtctcacca gagcgacatc
     3781 gaagtgaccg gcgactcctg caacaactac atgctgacct acaacaaggt ggagaacatg
     3841 accccaagag acctgggagc ttgcatcgac tgctccgccc gtcacatcaa cgctcaggtg
     3901 gccaagtctc acaacatcgc cctgatctgg aacgtgaagg acttcatgag cctgtccgag
     3961 cagctgcgca agcagatcag gagcgccgct aagaagaaca acctgccctt caagctgacc
     4021 tgcgctacca ccagacaggt ggtgaacgtg gtgaccacca agatcgccct gaagggcgga
     4081 tacccaactt tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc
     4141 aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc tgcagctctg
     4201 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac
     4261 aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg
     4321 ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg
     4381 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga
     4441 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga
     4501 gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat
     4561 ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca
     4621 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct
     4681 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg
     4741 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga
     4801 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt
     4861 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga
     4921 ggggaaatta ggccgggaag ccgatctcgg cttgaacgaa ttgttaggtg gcggtacttg
     4981 ggtcgatatc aaagtgcatc acttcttccc gtatgcccaa ctttgtatag agagccactg
     5041 cgggatcgtc accgtaatct gcttgcacgt agatcacata agcaccaagc gcgttggcct
     5101 catgcttgag gagattgatg agcgcggtgg caatgccctg cctccggtgc tcgccggaga
     5161 ctgcgagatc atagatatag atctcactac gcggctgctc aaacttgggc agaacgtaag
     5221 ccgcgagagc gccaacaacc gcttcttggt cgaaggcagc aagcgcgatg aatgtcttac
     5281 tacggagcaa gttcccgagg taatcggagt ccggctgatg ttgggagtag gtggctacgt
     5341 ctccgaactc acgaccgaaa agatcaagag cagcccgcat ggatttgact tggtcagggc
     5401 cgagcctaca tgtgcgaatg atgcccatac ttgagccacc taactttgtt ttagggcgac
     5461 tgccctgctg cgtaacatcg ttgctgctgc gtaacatcgt tgctgctcca taacatcaaa
     5521 catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacaaaa
     5581 aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc
     5641 aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagttt acgaaccgaa
     5701 caggcttatg tcaactgggt tcgtgccttc atccgtttcc acggtgtgcg tcacccggca
     5761 accttgggca gcagcgaagt cgaggcattt ctgtcctggc tggcgaacga gcgcaaggtt
     5821 tcggtctcca cgcatcgtca ggcattggcg gccttgctgt tcttctacgg caaggtgctg
     5881 tgcacggatc tgccctggct tcaggagatc ggaagacctc ggccgtcgcg gcgcttgccg
     5941 gtggtgctga ccccggatga agtggttcgc atcctcggtt ttctggaagg cgagcatcgt
     6001 ttgttcgccc aggactctag ctatagttct agtggttggc tactaatagg ttgtattgat
     6061 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     6121 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     6181 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt
     6241 aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga
     6301 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     6361 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     6421 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     6481 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     6541 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     6601 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     6661 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     6721 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     6781 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     6841 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     6901 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     6961 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     7021 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgctagc caggaagagt
     7081 ttgtagaaac gcaaaaaggc catccgtcag g
//
