LOCUS       sequence-352166-        4524 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4524)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC44A1_STOP
FEATURES             Location/Qualifiers
     source          1..4524
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2081..2180)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2197..2216)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2198..2216)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2221..2237)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2221..2237)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2339..2358)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2350..3159
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2417..2436)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3306..3894
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3795..3814
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4048..4065
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4224..4251)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4343..4429)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4478..4500
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4492..4509
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4493..4509
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggg atgctgtagc
      121 tccgcctcta gcgccgccca gtcctctaag agggagtgga agcctctgga ggaccgctcc
      181 tgcaccgata tcccatggct gctgctgttc atcctgtttt gcatcggcat gggcttcatc
      241 tgtggctttt ctatcgcaac aggagcagca gcaaggctgg tgagcggcta cgactcctat
      301 ggcaacatct gcggccagaa gaataccaag ctggaggcca tcccaaacag cggcatggat
      361 cacacacaga gaaagtacgt gttctttctg gacccctgta acctggatct gatcaatcgg
      421 aagatcaaga gcgtggccct gtgcgtggca gcatgtccca gacaggagct gaagaccctg
      481 tccgatgtgc agaagttcgc cgagatcaac ggctctgccc tgtgcagcta caatctgaag
      541 cctagcgagt ataccacatc cccaaagagc agcgtgctgt gcccaaagct gccagtgcct
      601 gcatccgccc caatcccctt ctttcacagg tgcgcccctg tgaacatctc ttgttacgcc
      661 aagttcgccg aggccctgat caccttcgtg agcgacaatt ctgtgctgca ccgcctgatc
      721 tctggcgtga tgacaagcaa ggagatcatc ctgggcctgt gcctgctgtc cctggtgctg
      781 tctatgatcc tgatggtcat catccggtac atcagcagag tgctggtgtg gatcctgacc
      841 atcctggtca tcctgggatc cctgggagga acaggcgtgc tgtggtggct gtatgccaag
      901 cagcggagaa gccccaagga gaccgtgaca cctgagcagc tgcagatcgc cgaggataac
      961 ctgcgggccc tgctgatcta cgccatctcc gccaccgtgt tcacagtgat cctgtttctg
     1021 atcatgctgg tcatgaggaa gcgcgtggcc ctgaccatcg ccctgttcca cgtggccggc
     1081 aaggtgttta tccacctgcc actgctggtg ttccagccct tttggacctt ctttgccctg
     1141 gtgctgttct gggtgtattg gatcatgaca ctgctgtttc tgggcaccac aggctctccc
     1201 gtgcagaatg agcagggctt cgtggagttt aagatcagcg gccctctgca gtacatgtgg
     1261 tggtatcacg tggtgggcct gatctggatc tccgagttca tcctggcatg tcagcagatg
     1321 accgtggcag gagcagtggt gacctactat ttcacacggg acaagagaaa cctgcctttt
     1381 acaccaatcc tggcctctgt gaataggctg atcaggtacc acctgggaac cgtggcaaag
     1441 ggcagcttta tcatcacact ggtgaagatc ccacggatga tcctgatgta tatccactcc
     1501 cagctgaagg gcaaggagaa cgcctgcgcc agatgcgtgc tgaagtcttg catctgctgt
     1561 ctgtggtgcc tggagaagtg tctgaattac ctgaaccaga atgcctatac cgccacagcc
     1621 atcaactcca ccaatttctg tacatctgcc aaggacgcct ttgtgatcct ggtggagaac
     1681 gccctgaggg tggccaccat caatacagtg ggcgatttca tgctgtttct gggcaaggtg
     1741 ctgatcgtgt gcagcaccgg actggcagga atcatgctgc tgaattacca gcaggattat
     1801 acagtgtggg tgctgcccct gatcatcgtg tgcctgttcg cctttctggt ggcccactgt
     1861 ttcctgtcca tctacgagat ggtggtggac gtgctgttcc tgtgctttgc catcgacacc
     1921 aagtacaacg atggctctcc tggccgcgag ttctatatgg ataaggtgct gatggagttt
     1981 gtggagaata gcaggaaggc catgaaggag gcaggcaagg gaggagtggc agacagcagg
     2041 gagctgaagc caatggcatc cggagcctct agcgcctaag acccagcttt cttgtacaaa
     2101 gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
     2161 caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg
     2221 gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca
     2281 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca
     2341 aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg
     2401 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca
     2461 atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt
     2521 agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg
     2581 cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact
     2641 gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat
     2701 attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt
     2761 ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt
     2821 ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg
     2881 aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc
     2941 tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga
     3001 gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt
     3061 tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat
     3121 aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg
     3181 taacactggc agagcattac gctgacttga cgggacggcg caagctcatg accaaaatcc
     3241 cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     3301 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     3361 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3421 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     3481 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3541 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3601 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3661 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3721 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3781 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3841 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3901 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     3961 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     4021 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     4081 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     4141 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
     4201 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
     4261 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
     4321 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     4381 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
     4441 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
     4501 acggccagtc ttaagctcgg gccc
//
