LOCUS       sequence-352167-        9208 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sars-CoV-2 protein mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9208)
  AUTHORS   Liu X, Huuskonen S, Laitinen T, Redchuk T, Bogacheva M, Salokas K, 
            Pohner I, Ohman T, Tonduru AK, Hassinen A, Gawriyski L, Keskitalo S,
            Vartiainen MK, Pietiainen V, Poso A, Varjosalo M
  TITLE     SARS-CoV-2-host proteome interactions for antiviral drug discovery.
  JOURNAL   Mol Syst Biol. 2021 Nov;17(11):e10396. doi: 10.15252/msb.202110396.
  PUBMED    34709727
REFERENCE   2  (bases 1 to 9208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2021-11"; volume: "17(11):e10396. doi"; pages: " 
            10.15252/msb.202110396"
COMMENT     Sequence Label: pDON207 NSP3 K977Q
FEATURES             Location/Qualifiers
     source          1..9208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(104..109,110..126,127..132))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      789..1377
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1278..1297
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1512..1539)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1631..1717)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    1770..1869
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(7708..7807)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(7919..7938)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(7997..8016)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(8590..9123)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
       61 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca
      121 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
      181 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc
      241 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg
      301 cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt
      361 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt
      421 gttcgcccag gactctagct atagttctag tggttggcta ctaataggtt gtattgatgt
      481 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
      541 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
      601 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa
      661 ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc
      721 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
      781 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
      841 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
      901 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
      961 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     1021 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     1081 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     1141 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     1201 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     1261 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     1321 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     1381 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     1441 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgctagcca ggaagagttt
     1501 gtagaaacgc aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag
     1561 tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg
     1621 ctcccggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa
     1681 aggcccagtc ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc
     1741 gcgttaacgc tagcatggat ctcgggcccc aaataatgat tttattttga ctgatagtga
     1801 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
     1861 aagttggcat ggcccctacc aaggtgacct tcggagacga caccgtgatc gaggtgcagg
     1921 gttacaagag cgtgaacatc accttcgagc tggacgaacg catcgacaag gtgctgaacg
     1981 aaaagtgctc cgcttacacc gtggagctgg gcaccgaggt gaacgaattc gcctgcgtgg
     2041 tggccgacgc tgtgatcaag accctgcagc cagtgtccga actgctgacc cctctgggta
     2101 tcgacctgga cgagtggtct atggctacct actacctgtt cgacgagagc ggcgaattca
     2161 agctggcctc tcacatgtac tgcagcttct accctcccga cgaagacgag gaagagggtg
     2221 actgcgaaga ggaagagttc gagccttcca ctcagtacga gtacggcacc gaagacgact
     2281 accagggcaa gcctctggaa ttcggagcca cctctgccgc tctgcagcct gaagaggaac
     2341 aggaggaaga ctggctggac gacgactccc agcagaccgt gggtcagcag gacggctctg
     2401 aagacaacca gaccaccacc atccagacca tcgtggaggt gcagcctcag ctggagatgg
     2461 aactgacccc agtggtgcag accatcgagg tgaacagctt ctccggctac ctgaagctga
     2521 ccgacaacgt gtacatcaag aacgctgaca tcgtggagga agccaagaag gtgaaaccaa
     2581 ccgtggtggt gaacgccgct aacgtgtacc tgaagcacgg cggaggtgtg gctggagctc
     2641 tgaacaaggc taccaacaac gccatgcagg tggaatccga cgactacatc gctaccaacg
     2701 gtcctctgaa ggtgggcgga tcctgcgtgc tgtctggcca caacctggcc aagcactgcc
     2761 tgcacgtggt gggaccaaac gtgaacaagg gagaggacat ccagctgctg aagagcgcct
     2821 acgaaaactt caaccagcac gaggtgctgc tggctccact gctgtctgcc ggaatcttcg
     2881 gtgctgatcc catccacagc ctgcgcgtgt gcgtggacac cgtgaggacc aacgtgtacc
     2941 tggccgtgtt cgacaagaac ctgtacgaca agctggtgag ctccttcctg gagatgaagt
     3001 ccgaaaagca ggtggagcag aagatcgccg agatccccaa ggaggaagtg aagcctttca
     3061 tcaccgaatc taagcccagc gtggagcaga gaaagcagga cgacaagaag atcaaggcct
     3121 gcgtggagga agtgaccacc accctggagg aaaccaagtt cctgaccgag aacctgctgc
     3181 tgtacatcga catcaacggc aacctgcacc ctgactccgc taccctggtg tctgacatcg
     3241 acatcacctt cctgaagaag gacgccccct acatcgtggg cgacgtggtg caggaaggag
     3301 tgctgaccgc tgtggtcatc cctaccaaga aggccggtgg caccaccgag atgctggcca
     3361 aggctctgcg taaggtgcca accgacaact acatcaccac ctaccccggt cagggcctga
     3421 acggttacac cgtggaggaa gctaagaccg tgctgaagaa gtgcaagtct gccttctaca
     3481 tcctgccctc catcatctct aacgagaagc aggaaatcct gggcaccgtg agctggaacc
     3541 tgcgcgaaat gctggcccac gctgaggaaa ccaggaagct gatgcccgtg tgcgtggaga
     3601 ccaaggccat cgtgagcacc atccagagaa agtacaaggg catcaagatc caggagggtg
     3661 tggtggacta cggcgctcgt ttctacttct acacctccaa gaccaccgtg gcctctctga
     3721 tcaacaccct gaacgacctg aacgagaccc tggtgaccat gccactggga tacgtgaccc
     3781 acggtctgaa cctggaggaa gccgctagat acatgcgttc cctgaaggtg cctgctaccg
     3841 tgagcgtgag cagccctgac gctgtgaccg cctacaacgg atacctgacc tcctctagca
     3901 agacccctga ggaacacttc atcgaaacca tcagcctggc cggttcctac aaggactggt
     3961 cttacagcgg acagtccacc cagctgggca tcgagttcct gaagcgcgga gacaagagcg
     4021 tgtactacac ctccaaccca accaccttcc acctggacgg tgaagtgatc accttcgaca
     4081 acctgaagac cctgctgtct ctgcgcgagg tgaggaccat caaggtgttc accaccgtgg
     4141 acaacatcaa cctgcacacc caggtggtgg acatgagcat gacctacgga cagcagttcg
     4201 gtcctaccta cctggacgga gctgacgtga ccaagatcaa gccacacaac tcccacgagg
     4261 gcaagacctt ctacgtgctg cctaacgatg acaccctgag agtggaggct ttcgaatact
     4321 accacaccac cgacccatcc ttcctgggca ggtacatgtc tgccctgaac cacaccaaga
     4381 agtggaagta cccccaggtg aacggactga ccagcatcaa gtgggccgac aacaactgct
     4441 acctggccac cgctctgctg accctgcagc agatcgaact gaagttcaac ccacccgctc
     4501 tgcaggacgc ttactacaga gcccgtgctg gcgaggccgc taacttctgc gctctgatcc
     4561 tggcctactg caacaagacc gtgggcgaac tgggagacgt gagagagacc atgagctacc
     4621 tgttccagca cgccaacctg gactcctgca agcgtgtgct gaacgtggtg tgcaagacct
     4681 gcggccagca gcagaccacc ctgaagggag tggaagctgt gatgtacatg ggtaccctgt
     4741 cctacgagca gttcaagaag ggcgtgcaga tcccttgcac ctgcggaaag caggccaccc
     4801 agtacctggt gcagcaggaa agcccattcg tgatgatgtc cgctcctcca gcccagtacg
     4861 aactgaagca cggcaccttc acctgcgctt ccgagtacac cggcaactac cagtgcggac
     4921 actacaagca catcacctct aaggagaccc tgtactgcat cgacggagcc ctgctgacca
     4981 agtcctctga atacaagggt cccatcaccg acgtgttcta caaggagaac agctacacca
     5041 ccaccatcaa gcccgtgacc tacaagctgg acggcgtggt gtgcaccgaa atcgacccta
     5101 agctggacaa ctactacaag aaggacaact cttacttcac cgagcagcct atcgacctgg
     5161 tgccaaacca gccctaccct aacgccagct tcgacaactt caagttcgtg tgcgacaaca
     5221 tcaagttcgc tgacgacctg aaccagctga ccggatacaa gaagcctgcc tctagagagc
     5281 tgaaggtgac cttcttccca gacctgaacg gcgacgtggt ggctatcgac tacaagcact
     5341 acaccccaag cttcaagaag ggagccaagc tgctgcacaa gcccatcgtg tggcacgtga
     5401 acaacgctac caacaaggcc acctacaagc ctaacacctg gtgcatccgt tgcctgtggt
     5461 ctaccaagcc agtggaaacc tccaactctt tcgacgtgct gaagagcgag gacgctcagg
     5521 gcatggacaa cctggcctgc gaagacctga agcccgtgag cgaggaagtg gtggagaacc
     5581 caaccatcca gaaggacgtg ctggaatgca acgtgaagac caccgaggtg gtgggagaca
     5641 tcatcctgaa gccagctaac aacagcctga agatcaccga ggaagtgggt cacaccgacc
     5701 tgatggccgc ttacgtggac aacagctccc tgaccatcaa gaagcccaac gagctgagca
     5761 gggtgctggg tctgaagacc ctggctactc acggtctggc tgctgtgaac tctgtgccat
     5821 gggacaccat cgctaactac gccaagcctt tcctgaacaa ggtggtgtcc accaccacca
     5881 acatcgtgac cagatgtctg aaccgcgtgt gcaccaacta catgccctac ttcttcaccc
     5941 tgctgctgca gctgtgcacc ttcaccagaa gcaccaactc ccgtatcaag gctagcatgc
     6001 ctaccaccat cgccaagaac accgtgaaga gcgtgggaaa gttctgcctg gaggcttcct
     6061 tcaactacct gaagtctcca aacttcagca agctgatcaa catcatcatc tggttcctgc
     6121 tgctctccgt gtgcctgggt tccctgatct actctaccgc cgctctggga gtgctgatgt
     6181 ctaacctggg tatgcctagc tactgcactg gttacagaga gggatacctg aacagcacca
     6241 acgtgaccat cgccacctac tgcaccggca gcatcccatg ctccgtgtgc ctgtctggac
     6301 tggacagcct ggacacctac ccctccctgg aaaccatcca gatcaccatc tctagcttca
     6361 agtgggacct gaccgccttc ggtctggtgg ctgagtggtt cctggcctac atcctgttca
     6421 ccaggttctt ctacgtgctg ggcctggccg ctatcatgca gctgttcttc tcctacttcg
     6481 ctgtgcactt catcagcaac tcctggctga tgtggctgat catcaacctg gtgcagatgg
     6541 ctcccatctc tgccatggtg cgcatgtaca tcttcttcgc cagcttctac tacgtgtgga
     6601 agtcctacgt gcacgtggtg gacggttgca actcctctac ctgcatgatg tgctacaagc
     6661 gcaacagggc taccagggtg gagtgcacca ccatcgtgaa cggcgtgcgc aggtccttct
     6721 acgtgtacgc caacggcggc aagggattct gcaagctgca caactggaac tgcgtgaact
     6781 gcgacacctt ctgcgctggc tccaccttca tctctgacga ggtggccaga gacctgagcc
     6841 tgcagttcaa gcgtccaatc aaccccaccg accagagctc ctacatcgtg gactctgtga
     6901 ccgtgaagaa cggtagcatc cacctgtact tcgacaaggc tggccagaag acctacgagc
     6961 gccactctct gagccacttc gtgaacctgg acaacctgag ggccaacaac accaagggca
     7021 gcctgcctat caacgtgatc gtgttcgacg gaaagtccaa gtgcgaggaa tctagcgcca
     7081 agtccgccag cgtgtactac agccagctga tgtgccagcc aatcctgctg ctggaccagg
     7141 ctctggtgtc tgacgtggga gacagcgccg aagtggccgt gaagatgttc gacgcctacg
     7201 tgaacacctt ctcctctacc ttcaacgtgc ctatggagaa gctgaagacc ctggtggcta
     7261 ctgctgaggc tgaactggct aagaacgtgt ccctggacaa cgtgctgagc accttcatct
     7321 ccgccgctag gcagggtttc gtggactctg acgtggaaac caaagacgtg gtggagtgcc
     7381 tgaagctgtc tcaccagagc gacatcgaag tgaccggcga ctcctgcaac aactacatgc
     7441 tgacctacaa caaggtggag aacatgaccc caagagacct gggagcttgc atcgactgct
     7501 ccgcccgtca catcaacgct caggtggcca agtctcacaa catcgccctg atctggaacg
     7561 tgaaggactt catgagcctg tccgagcagc tgcgcaagca gatcaggagc gccgctaaga
     7621 agaacaacct gcccttcaag ctgacctgcg ctaccaccag acaggtggtg aacgtggtga
     7681 ccaccaagat cgccctgaag ggcggatacc caactttctt gtacaaagtt ggcattataa
     7741 gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca
     7801 ttatttgcca tccagctgca gctctggccc gtgtctcaaa atctctgatg ttacattgca
     7861 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca
     7921 aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg
     7981 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca
     8041 atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt
     8101 agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg
     8161 cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact
     8221 gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat
     8281 attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt
     8341 ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt
     8401 ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg
     8461 aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc
     8521 tcacttgata accttatttt tgacgagggg aaattaggcc gggaagccga tctcggcttg
     8581 aacgaattgt taggtggcgg tacttgggtc gatatcaaag tgcatcactt cttcccgtat
     8641 gcccaacttt gtatagagag ccactgcggg atcgtcaccg taatctgctt gcacgtagat
     8701 cacataagca ccaagcgcgt tggcctcatg cttgaggaga ttgatgagcg cggtggcaat
     8761 gccctgcctc cggtgctcgc cggagactgc gagatcatag atatagatct cactacgcgg
     8821 ctgctcaaac ttgggcagaa cgtaagccgc gagagcgcca acaaccgctt cttggtcgaa
     8881 ggcagcaagc gcgatgaatg tcttactacg gagcaagttc ccgaggtaat cggagtccgg
     8941 ctgatgttgg gagtaggtgg ctacgtctcc gaactcacga ccgaaaagat caagagcagc
     9001 ccgcatggat ttgacttggt cagggccgag cctacatgtg cgaatgatgc ccatacttga
     9061 gccacctaac tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctgcgtaa
     9121 catcgttgct gctccataac atcaaacatc gacccacggc gtaacgcgct tgctgcttgg
     9181 atgcccgagg catagactgt acaaaaaa
//
