LOCUS       sequence-352168-        4290 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4290)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC15A5_STOP
FEATURES             Location/Qualifiers
     source          1..4290
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1847..1946)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1963..1982)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1964..1982)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1987..2003)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1987..2003)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2105..2124)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2116..2925
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2183..2202)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3072..3660
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3561..3580
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3814..3831
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3990..4017)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4109..4195)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4244..4266
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4258..4275
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4259..4275
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgag cgtgaccggc
      121 ttcaccatca cagacgagaa ggtgcacctg taccactcca tcgagaagga gaagacagtg
      181 aggcacatcg gcgatctgtg cagctcccac agcgtgaaga agatccaagt gggcatctgc
      241 ctgctgctgg tggagctgtg cgagcggttc accttcttcg aggtggtgtg caacatgatc
      301 cctttctgta caatcaagct gggctaccac aactgccagg ccgccatcct gaacctgtgc
      361 tttatcggca cctccatcct gacaccagtg ttcgtgaggt ggctgaccga cgtgtacctg
      421 ggccgcaata agctggtgta tatctgtctg ttcctgcact ttctgggaac agccctgctg
      481 agcgtggtgg catttcccct ggaggatttc tacctgggca cctatcacgc cgtgaacaat
      541 atccctaaga cagagcagca caggctgttt tacgtggccc tgctgaccat ctgcctggga
      601 atcggaggcg tgcgcgcaat cgtgtgcccc ctgggagcct tcggcctgca ggagtacgga
      661 agccagaaga ccatgtcctt ctttaactgg ttttattggc tgatgaacct gaatgccaca
      721 atcgtgttcc tgggcatctc ttacatccag cacagccagg catgggcact ggtgctgctg
      781 atcccattca tgtccatgct gatggccgtg atcaccctgc acatgatcta ctataacctg
      841 atctatcagt ccgagaagcg gtgctctctg ctgaccggag tgggcgtgct ggtgtctgcc
      901 ctgaagacat gccaccccca gtactgtcac ctgggcagag acgtgacctc ccagctggat
      961 cacgccaagg agaagaatgg cggctgctat tctgagctgc acgtggagga caccacattc
     1021 tttctgaccc tgctgccact gttcatcttt cagctgctgt accggatgtg catcatgcag
     1081 atcccctctg gctactatct gcagacaatg aacagcaatc tgaacctgga tggcttcctg
     1141 ctgcccatcg ccgtgatgaa cgccatctct agcctgccac tgctgatcct ggcccccttt
     1201 ctggagtatt tcagcacctg cctgtttcct tccaagcggg tgggctcttt cctgagcaca
     1261 tgtatcatcg ccggcaatct gtttgccgcc ctgtctgtga tgatcgccgg cttctttgag
     1321 atccacagaa agcacttccc agcagtggag cagcctctga gcggcaaggt gctgaccgtg
     1381 tcctctatgc cttgctttta cctgatcctg cagtatgtgc tgctgggcgt ggccgagaca
     1441 ctggtgaacc ctgccctgtc cgtgatctct taccggtttg tgccatctaa tgtgagaggc
     1501 accagcatga acttcctgac actgttcaat ggctttggct gtttcaccgg cgccctgctg
     1561 gtgaagctgg tgtatctgat ctccgacggc aactggttcc caaataccct gaacaagggc
     1621 aatctggaga gcttcttttt ctttctggcc tccctgacac tgctgaatgt gctgggcttt
     1681 tgcagcgtgt cccagcggta ctgtaatctg aaccacttca acgcccagaa tatcagaggc
     1741 agcaacctgg aggagaccct gctgctgcac gagaagtccc tgaagtttta tggctctatc
     1801 caggagttca gctcctctat cgatctgtgg gagacagccc tgtaagaccc agctttcttg
     1861 tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact
     1921 atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat
     1981 tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac
     2041 attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt
     2101 aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc
     2161 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt
     2221 gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc
     2281 aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa
     2341 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc
     2401 accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt
     2461 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt
     2521 aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat
     2581 aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa
     2641 gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt
     2701 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt
     2761 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt
     2821 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat
     2881 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat
     2941 tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca
     3001 aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc
     3061 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     3121 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     3181 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     3241 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3301 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     3361 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3421 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3481 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3541 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3601 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3661 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3721 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3781 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     3841 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     3901 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc
     3961 gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct
     4021 tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct
     4081 tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca
     4141 aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc
     4201 tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgttgt
     4261 aaaacgacgg ccagtcttaa gctcgggccc
//
