LOCUS       sequence-35217-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  TITLE     LIC N-terminal fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET His6 Mocr TEV LIC cloning vector (1O)
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(25..42)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(108..128)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(498..515)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             534..556
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    571..595
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(595..614)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(596..614)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(698..717)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg cggccgcaag
       61 cttgtcgacg gcgctcgaat tcggatccgt tatccacttc caatattgga ttggaagtac
      121 aggttttcct cgatccccgt cccctcttca tcttcctcat actcctccac ttcttccagg
      181 tattcattca ggagatcttc cgcatcttcc cataaatcga tggtaagttg ttcgtagatc
      241 cgcgcttgca gaattctgat gtcgtccttc gtatctggca tgaggccact atcctcaaat
      301 tccagatcaa taccttcaga tgccataact gaccgaatat cggcataata gtgagggacg
      361 gcattatcgg cggccatatg aattgcgtcg tgaaggtcat ctgtatcccg tatgtcatca
      421 taacggatgt tctccttaag catttcgtac gcgtggtcga aaacattatt gtatgtcatg
      481 ttgctcatag aagaaccatg gtgatggtga tggtgagaag aacccatggt atatctcctt
      541 cttaaagtta aacaaaatta tttctagagg ggaattgtta tccgctcaca attcccctat
      601 agtgagtcgt attaatttcg cgggatcgag atctcgatcc tctacgccgg acgcatcgtg
      661 gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat
      721 ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg
      781 gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg
      841 gcggcggtgc tcaacggcct caacctacta ctgggctgct tcct
//
