LOCUS       sequence-352170-        9328 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encoding a part of the rapamycin-induced Ras activation system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9328)
  AUTHORS   Lin S, Hirayama D, Maryu G, Matsuda K, Hino N, Deguchi E, Aoki K, 
            Iwamoto R, Terai K, Matsuda M
  TITLE     Redundant roles of EGFR ligands in the ERK activation waves during 
            collective cell migration.
  JOURNAL   Life Sci Alliance. 2021 Oct 19;5(1). pii: 5/1/e202101206. doi: 
            10.26508/lsa.202101206. Print 2022 Jan.
  PUBMED    34667080
REFERENCE   2  (bases 1 to 9328)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2021-10-19"; volume: "5(1). pii: 5/1/e202101206. 
            doi"; pages: " 10.26508/lsa.202101206. Print 2022 Jan"
COMMENT     Sequence Label: pT2Aneo-mRFP-FKBP-mSos1-linkercat
FEATURES             Location/Qualifiers
     source          1..9328
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        20..124
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(125..193,194..985)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(343..362)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1156..1744
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1645..1664
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1898..1915
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2032..2053
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2068..2073,2074..2091,2092..2098)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2106..2122
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2111..2133
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2130..2146
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2130..2146
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2165..2185
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2167..2185
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    join(2242..2253,2254..2441)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     enhancer        2463..2842
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2844..3121
                     /label=chicken beta-actin promoter
     intron          3122..4138
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4062..4084
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4146..4165
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             4205..4879
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             4205..4879
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(4335..4353)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4603..4622
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4797..4820
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             4907..5227
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     misc_feature    6797..7346
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6941..6958)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7168..7187
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7349..8143
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7403..7422)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8013..8032
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             8150..8170
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8221..8355
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8245..8264)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8299..8318
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(8477..8639,8640..8651)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(8681..8700)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8682..8700)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8710..8727)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8710..8726)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8719..8741)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8868..9295
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8954..8973)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9164..9185
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
       61 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      121 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      181 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
      241 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
      301 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
      361 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
      421 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
      481 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
      541 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
      601 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
      661 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
      721 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
      781 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
      841 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
      901 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
      961 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     1021 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1081 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1141 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1201 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1261 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     1321 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1381 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1441 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1501 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1561 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1621 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1681 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1741 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     1801 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     1861 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     1921 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     1981 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     2041 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     2101 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     2161 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctcggatctg ctgggcttgc
     2221 tgaaggtagg gggtcaagaa ccagaggtgt aaagtacttg agtaatttta cttgattact
     2281 gtacttaagt attatttttg gggattttta ctttacttga gtacaattaa aaatcaatac
     2341 ttttactttt acttaattac atttttttag aaaaaaaagt actttttact ccttacaatt
     2401 ttatttacag tcaaaaagta cttatttttt ggagatcact tgggccctct agatgcatgc
     2461 tcgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
     2521 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     2581 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     2641 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     2701 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     2761 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     2821 attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat
     2881 ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc
     2941 gatgggggcg gggggggggg gggggcgcgc gccrggsggg gsggggsggg gsgrggggsg
     3001 gggsggggsg rggcggrgag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
     3061 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
     3121 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
     3181 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
     3241 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
     3301 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
     3361 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
     3421 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     3481 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     3541 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     3601 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     3661 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     3721 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     3781 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     3841 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     3901 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     3961 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     4021 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     4081 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     4141 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcgccctttc
     4201 tagaatggcc tcctccgagg acgtcatcaa ggagttcatg cgcttcaagg tgcgcatgga
     4261 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     4321 gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga
     4381 catcctgtcc cctcagttcc agtacggctc caaggcctac gtgaagcacc ccgccgacat
     4441 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     4501 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
     4561 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
     4621 gaccatgggc tgggaggcct ccaccgagcg gatgtacccc gaggacggcg ccctgaaggg
     4681 cgagatcaag atgaggctga agctgaagga cggcggccac tacgacgccg aggtcaagac
     4741 cacctacatg gccaagaagc ccgtgcagct gcccggcgcc tacaagaccg acatcaagct
     4801 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgagc gcgccgaggg
     4861 ccgccactcc accggcgccg tcgacctcga caagagtgct ggtggtggag tgcaggtgga
     4921 aaccatctcc ccaggagacg ggcgcacctt ccccaagcgc ggccagacct gcgtggtgca
     4981 ctacaccggg atgcttgaag atggaaagaa atttgattcc tcccgggaca gaaacaagcc
     5041 ctttaagttt atgctaggca agcaggaggt gatccgaggc tgggaagaag gggttgccca
     5101 gatgagtgtg ggtcagagag ccaaactgac tatatctcca gattatgcct atggtgccac
     5161 tgggcaccca ggcatcatcc caccacatgc cactctcgtc ttcgatgtgg agcttctaaa
     5221 actggaaagt gctggtggta gtgctggtgg tagtgctggt ggtagtgctg gtggtagtgc
     5281 tggtggtctc gagaccctgg agaggatgct ggacgtaacg gtgctgcagg aggagaagga
     5341 ggagcagatg aggctgccca gtgctgaagt gtacaggttt gcagaacctg actccgagga
     5401 gaatattcta ttcgaagaga atgtgcagcc caaagctggg atccccatta tcaaggcagg
     5461 gacagtgctt aagctcattg agaggcttac ctaccacatg tacgcagatc caaattttgt
     5521 tcggacgttt cttacaacat acaggtcctt ttgcagacct caagaactac tgagtcttct
     5581 gatagaaaga tttgaaattc cagagcctga gccaacagaa gctgatcgca tagctataga
     5641 gaatggagat cagcccctga gtgcagagct gaagaggttt agaaaggaat atattcagcc
     5701 tgtgcagttg agggtgttaa atgtgtgtcg gcactgggtg gagcaccatt tctatgactt
     5761 tgaaagagat gcagaccttt tacagagaat ggaggaattt attggaacag taagaggtaa
     5821 agcaatgaaa aaatgggtcg aatccatcac taagataatc caaaggaaaa aaattgcaag
     5881 agacaatggc ccaggtcata acattacatt tcagagctca cctcccacag ttgagtggca
     5941 cataagcaga cctgggcaca tagagacttt tgacttgctc accttacacc caatagaaat
     6001 tgctcggcaa ctcactttac ttgaatcaga tctataccgg gctgtgcagc catcagaatt
     6061 agttggaagt gtgtggacaa aagaagataa agaaattaat tctcccaacc ttctgaagat
     6121 gattcggcac accactaacc tcactttgtg gtttgagaaa tgtattgtag aaacagaaaa
     6181 cttagaagaa agagtagctg tagtaagtcg gataattgag attctacaag tctttcaaga
     6241 gctgaacaac ttcaatggtg tcctggaagt tgtcagtgct atgaactcgt cacctgttta
     6301 cagactagac cacacatttg agcaaatacc aagcagacaa aagaaaattt tagaagaagc
     6361 tcatgaattg agtgaagatc actataagaa atatttggca aaactcaggt ctattaatcc
     6421 accgtgtgtg cctttctttg gaatttatct cacaaatatc ctgaagacag aagagggcaa
     6481 ccctgaggtc ctgaggagac acgggaaaga gcttattaac ttcagcaaga ggaggagagt
     6541 ggccgagatc acaggcgaga tccagcagta ccagaaccag ccctactgct tacgggtgga
     6601 gccggacatc aagaggttct ttgaaaactt gaatccaatg ggaaacagca tggagaaaga
     6661 atttacagac tatctgttca acaaatccct agaaatagaa ccccggcacg gcggccgcgg
     6721 ctctagagga tccgttaact aacttaagct agcaacggtt tccctctagc gggatcaatt
     6781 ccgccccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
     6841 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
     6901 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
     6961 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
     7021 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
     7081 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
     7141 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
     7201 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc
     7261 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg
     7321 gttttccttt gaaaaacacg ataataccat gattgaacaa gatggattgc atgcaggttc
     7381 gccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     7441 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     7501 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     7561 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     7621 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     7681 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     7741 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     7801 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     7861 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     7921 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     7981 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     8041 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     8101 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgaactagac ctaagaagaa
     8161 gcgtaaggtg taagtcgacg gccgccaccg cggtggagct cgaattaatt catcgatgat
     8221 gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     8281 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     8341 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     8401 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat
     8461 cctctagatc agatctttct tgcttttact tttacttcct taatactcaa gtacaatttt
     8521 aatggagtac ttttttactt ttactcaagt aagattctag ccagatactt ttacttttaa
     8581 ttgagtaaaa ttttccctaa gtacttgtac tttcacttga gtaaaatttt tgagtacttt
     8641 ttacacctct gtcaagaacc atatgccggt acccaattcg ccctatagtg agtcgtatta
     8701 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     8761 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     8821 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     8881 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccacca cacccgccgc
     8941 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     9001 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     9061 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     9121 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     9181 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     9241 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     9301 aaaatattaa cgcttacaat ttaggtgg
//
