LOCUS       sequence-352175-        3825 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC22A18_STOP
FEATURES             Location/Qualifiers
     source          1..3825
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1382..1481)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1498..1517)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1499..1517)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1522..1538)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1522..1538)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1640..1659)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1651..2460
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1718..1737)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2607..3195
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3096..3115
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3349..3366
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3525..3552)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3644..3730)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3779..3801
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3793..3810
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3794..3810
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgca gggagcaagg
      121 gcacctagag accagggccg gagcccagga agaatgtccg ccctgggcag gagctccgtg
      181 atcctgctga cctacgtgct ggccgccacc gagctgacat gcctgttcat gcagttttct
      241 atcgtgccct acctgagcag gaagctgggc ctggacagca tcgcattcgg ttaccttcaa
      301 acgacttttg gcgtgctgca gctgctggga ggacccgtgt tcggccgctt tgcagatcag
      361 aggggagcaa gggccgccct gacactgtcc ttcctggccg ccctggccct gtacctgctg
      421 ctggcagcag catctagccc tgccctgcca ggcgtgtatc tgctgtttgc atccaggctg
      481 ccaggcgccc tgatgcacac cctgccagca gcacagatgg tcatcacaga tctgtctgcc
      541 ccagaggaga ggccagccgc cctgggcaga ctgggactgt gcttcggagt gggcgtgatc
      601 ctgggaagcc tgctgggagg caccctggtg tccgcctacg gaatccagtg ccctgcaatc
      661 ctggccgccc tggccaccct gctgggagcc gtgctgtcct ttacatgtat ccctgcctct
      721 accaagggcg ccaagacaga cgcacaggca ccactgcctg gaggaccacg ggcctccgtg
      781 ttcgacctga aggccatcgc ctctctgctg aggctgcccg atgtgcctcg catctttctg
      841 gtgaaggtgg ccagcaactg tcccaccggc ctgttcatgg tcatgttttc catcatctct
      901 atggatttct ttcagctgga ggcagcacag gcaggatatc tgatgtcctt ctttggcctg
      961 ctgcagatgg tgacacaggg cctggtcatc ggccagctgt cctctcactt ctctgaggag
     1021 gtgctgctga gagccagcgt gctggtgttt atcgtggtgg gcctggccat ggcctggatg
     1081 agctccgtgt tccacttttg cctgctggtg ccaggcctgg tgttcagcct gtgcaccctg
     1141 aacgtggtga cagactccat gctgatcaag gccgtgtcta ccagcgatac cggcacaatg
     1201 ctgggactgt gcgcatctgt gcagccactg ctgcggaccc tgggccctac agtgggcggc
     1261 ctgctgtata gaagcttcgg cgtgcccgtg tttggccacg tgcaggtggc catcaataca
     1321 ctggtgctgc tggtgctgtg gcggaagcca atgccccaga ggaaggacaa ggtgcgctaa
     1381 gacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc
     1441 aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc
     1501 tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa
     1561 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct
     1621 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg
     1681 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
     1741 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg
     1801 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     1861 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
     1921 gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa
     1981 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
     2041 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg
     2101 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
     2161 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca
     2221 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     2281 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     2341 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     2401 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
     2461 tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc
     2521 gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
     2581 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2641 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     2701 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     2761 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     2821 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     2881 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     2941 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     3001 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     3061 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     3121 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     3181 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     3241 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     3301 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     3361 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     3421 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     3481 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca
     3541 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc
     3601 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga
     3661 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc
     3721 gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc
     3781 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccc
//
