LOCUS       sequence-352191-        8973 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8973)
  AUTHORS   Yu X, Zhan X, D'Costa J, Tanavde VM, Ye Z, Peng T, Malehorn MT, Yang
            X, Civin CI, Cheng L
  TITLE     Lentiviral vectors with two independent internal promoters transfer 
            high-level expression of multiple transgenes to human hematopoietic 
            stem-progenitor cells.
  JOURNAL   Mol Ther. 2003 Jun . 7(6):827-38.
  PUBMED    12788657
REFERENCE   2  (bases 1 to 8973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2003 Jun . 7(6):827-38."
COMMENT     Sequence Label: EF.delmFasL.CMV.GFP
FEATURES             Location/Qualifiers
     source          1..8973
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    12..33
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(48..53,54..71,72..78)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    86..102
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     91..113
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     110..126
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     110..126
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     145..165
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        147..165
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        193..419
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             420..600
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    647..772
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1265..1498
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1683..1727
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2115..3293
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2346..3284
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3241..3261
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        3310..3328
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        4262..4565
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4566..4769
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4720..4740
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      4851..4860
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4857..4859,4860..4862,4863..5576)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4857..4859,4860..4862,4863..5573)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4902..4923)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5163..5182)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5510..5531
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5684..5917
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5989..6110
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6026..6045)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6080..6099
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6150..6285
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(6306..6324)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6334..6351)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6334..6350)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6343..6365)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6492..6947
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6579..6598)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6789..6810
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6973..7077
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7078..7146,7147..7938)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7296..7315)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8109..8697
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8598..8617
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8851..8868
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
       61 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
      121 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag
      181 ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga
      241 gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa
      301 ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa
      361 ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg
      421 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
      481 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
      541 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
      601 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
      661 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
      721 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
      781 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
      841 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
      901 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
      961 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     1021 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     1081 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
     1141 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     1201 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     1261 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     1321 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     1381 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     1441 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     1501 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     1561 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     1621 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     1681 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     1741 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     1801 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     1861 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     1921 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac
     1981 ggtatcgatc acgagactag cctcgagaag cttgatgacg gtatcgataa gctttgcaaa
     2041 gatggataaa gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga
     2101 aaggagtggg aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc
     2161 ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg
     2221 taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac
     2281 cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa
     2341 cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt
     2401 gcgtgccttg aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg
     2461 ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt
     2521 gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg
     2581 cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga
     2641 cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt
     2701 cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga
     2761 ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc
     2821 ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg
     2881 cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga
     2941 gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga
     3001 aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt
     3061 ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg
     3121 ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt
     3181 ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc
     3241 tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgaggaatta
     3301 gcttggtact aatacgactc actataggga gacccaagct ggctaggtaa gcttgataat
     3361 tcccaccatg cagcagccca tgaattaccc atgtccccag atcttctggg tagacagcag
     3421 tgccacttca tcttgggctc ctccagggtc agtttttccc tgtccatctt gtgggcctag
     3481 agggccggac caaaggagac cgccacctcc accaccacct gtgtcaccac taccaccgcc
     3541 atcacaacca ctcccactgc cgccactgac ccctctaaag aagaaggacc acaacacaaa
     3601 tctgtggcta ccggtggtat ttttcatggt tctggtggct ctggttggaa tgggattagg
     3661 aatgtatcag ctcttccacc tgcagaagga actggcagaa ctccgtgagg ccaaccccag
     3721 tacaccctct gaaaaaaaag agccgaggag tgtggcccat ttaacaggga acccccactc
     3781 aaggtccatc cctctggaat gggaagacac atatggaacc gctctgatct ctggagtgaa
     3841 gtataagaaa ggtggccttg tgatcaacga aactgggttg tacttcgtgt attccaaagt
     3901 atacttccgg ggtcagtctt gcaacaacca gcccctaaac cacaaggtct atatgaggaa
     3961 ctctaagtat cctgaggatc tggtgctaat ggaggagaag aggttgaact actgcactac
     4021 tggacagata tgggcccaca gcagctacct gggggcagta ttcaatctta ccagtgctga
     4081 ccatttatat gtcaacatat ctcaactctc tctgatcaat tttgaggaat ctaagacctt
     4141 tttcggcttg tataagcttt aaggtacctc tagagtcgac ccgggcggcc atcgaattcc
     4201 tgcagcccta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     4261 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     4321 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     4381 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     4441 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     4501 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     4561 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     4621 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     4681 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     4741 tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagcgct
     4801 accggactca gatctcgagc tcaagcttcg aattggggga tccaccggtc gccaccatgg
     4861 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     4921 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     4981 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     5041 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     5101 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     5161 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     5221 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     5281 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     5341 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     5401 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     5461 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     5521 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg
     5581 gccgcgtcga cgggcccgcg gaattcgagc tcggtacctt taagaccaat gacttacaag
     5641 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     5701 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     5761 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     5821 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     5881 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     5941 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     6001 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     6061 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     6121 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     6181 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     6241 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa
     6301 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
     6361 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     6421 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     6481 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     6541 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     6601 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     6661 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     6721 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     6781 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     6841 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     6901 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     6961 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     7021 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     7081 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     7141 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     7201 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     7261 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     7321 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     7381 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     7441 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     7501 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     7561 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     7621 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     7681 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     7741 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     7801 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     7861 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     7921 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     7981 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     8041 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     8101 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     8161 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     8221 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     8281 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     8341 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     8401 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     8461 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     8521 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     8581 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     8641 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     8701 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     8761 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     8821 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     8881 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     8941 gacaggtttc ccgactggaa agcgggcagt gag
//
