LOCUS       sequence-352194-       10876 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of  V5-tagged mouse Tmem214.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10876)
  AUTHORS   Cheng LC, Zhang X, Baboo S, Nguyen JA, Martinez-Bartolome S, Loose 
            E, Diedrich J, Yates JR 3rd, Gerace L
  TITLE     Comparative membrane proteomics reveals diverse cell regulators 
            concentrated at the nuclear envelope.
  JOURNAL   Life Sci Alliance. 2023 Jul 11;6(9):e202301998. doi: 
            10.26508/lsa.202301998. Print 2023 Sep.
  PUBMED    37433644
REFERENCE   2  (bases 1 to 10876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2023-07-11"; volume: "6(9):e202301998. doi"; 
            pages: " 10.26508/lsa.202301998. Print 2023 Sep"
COMMENT     Sequence Label: pLV V5-Tmem214
FEATURES             Location/Qualifiers
     source          1..10876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2221..2432
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2433..3371
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3328..3348
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3387..3396
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3396..3437
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     regulatory      3457..3466
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    5670..6219
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5814..5831)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6041..6060
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6222..6821
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6222..6241)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6718..6738
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6845..7433
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6898..6918)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7316..7327)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7640..8273
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8401..8417)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8401..8417)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8414..8436)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8425..8441
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8449..8455,8456..8473,8474..8479))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8494..8515
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8632..8649)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8803..9391)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8883..8902)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9562..10353,10354..10422))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10185..10204
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10423..10527)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10575..10709
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10612..10631)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10666..10685
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatccgccg ccatgggtaa gcctatccct aaccctctcc
     3421 tcggtctcga ttctacgggg ggcggagggg gtgggcccat ggcggccaga tcagcgggta
     3481 gcggtggctg ggaggtggtg aagaggggcc ggcgacctgg agcaagcagc ggtgggcgag
     3541 gcggcggcgg tggcagtgac cgccgggcgc tcggggaggc aaatggagtg ttgaaatacg
     3601 acctgagctc accaatccag actacaagca ctctttatga gcgtggcttt gagaaaatca
     3661 tgaaacggca gaataaagag caagttccac cccctgctgc agagtctaag aaaccgataa
     3721 acaagaagca accaaagaag gtgacagctg ttcctagcca aaaccagaaa cagggcccat
     3781 tccgacgcct ggaggacgca ctgaaagcgc tggatgtggc agctctgcag aaggaactgg
     3841 acaagagcca gagcgtgttc actgggaacc cctccgtgtg gctgaaggac ctggccagct
     3901 atctcaacta caagctccag actccacgga tggagcccac tctgagccag tatccacatg
     3961 attatcccta cagccttgtg agccgagagc tacgtgggat catccggggg ctcctgacca
     4021 aagctgcagg gtctgtggag ctcttttttg accactgtct attcaccatg ctgcaagagc
     4081 tggataaaac accaggggag tcgctacatg ggtaccgcat ctgtattcaa gccgttctgc
     4141 aagacaagcc taagattgtc acctcaaacc tggacaagtt cctagagctt ctgcggtccc
     4201 accagagccg accagcaaag tgcctgacca tcatgtgggc cctggggcaa gcgggtttca
     4261 ccaacctcac tgagggactg aaagtgtggc tggggatcat gctgcctgtg ctgggcatca
     4321 aggctctgtc tccctttgcc atcgcatacc tggaccggct gctcctgatg catcccaacc
     4381 tcaccaaagg ctttggcatg attggcccca aggacttctt cccccttctg gactttgcct
     4441 atatgcccaa caattccctg agccccagcc tgcaggagca gctgtgccag ctcttccccc
     4501 gattgaaggt gctggcattt ggggccaagc cggaatcttc cctgcacacc tacttcccgt
     4561 cattcctgtc cagagccacc cctagctgtc ctgctgccat gaagaaagag ctcctggcca
     4621 gcctgaccca gtgcctgact gtggaccccc ttagcactag tgtctggaga cagctgtacc
     4681 ccaagcatct gtcacagtcc agcctgctgc tggagcacct actcaagtcc tgggagcaca
     4741 tccccaagaa ggcacggaaa tctttacaag aaaccatcca gtccttgaag gttaccaacc
     4801 aggagcttct gaagaagggc agtggtggca gtgagcatgt tctcacctgt gacacagcct
     4861 gcaagggctt gttgcaacgg gcacggggtc ctcggccacc ctgggcccgg ctattcctgc
     4921 tgcttctggt ctttgcagta ggattcctat gtcatgactt gcggtcaaac agctctttgc
     4981 aggcctccct cactggccgg ctgcttcgat cctccggtct cttgcctgtt ggccagcaag
     5041 tgtgtgcgag actctcctcc tacagcctgc agagctacaa ctggctgcag gagaccttgc
     5101 cagcctgtgg ctcccacctg cttgctgtgg tacagcccag tctgcagctg gcctggacac
     5161 acatctatgc catattcagc ttcctttctg cccactgtgc ctcctacctt gcctgcttca
     5221 gtgatagcct ggctggcttc tttcagaggg tccagctccc tgaggccctg cagcagctct
     5281 tccacgcctt gaaggaactg ctgctgctct tctgccatag cgtgctgctg cccacatggc
     5341 acttgctgct tgcagccctg gcccaagtcc aggagcattg ccatgaggcc tgcagagggg
     5401 acgtgacctg ggactgcatt aagacacagt tgagcagagc tgcccaatgg acctggctct
     5461 gcctgcagga tgtcacagtg gctttcttgg actgggcact caccatgata tcccagcaat
     5521 aatgggccgc gacgatgttt aaacttcgaa acgcgtgaat tcctcgaggg cggccgctct
     5581 agagtcgacg ggccgcggta acaattgtta actaacttaa gctagcaacg gtttccctct
     5641 agcgggatca attccgcccc ccccccctaa cgttactggc cgaagccgct tggaataagg
     5701 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
     5761 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
     5821 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
     5881 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
     5941 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
     6001 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
     6061 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
     6121 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa
     6181 ccacggggac gtggttttcc tttgaaaaac acgataatac catgaccgag tacaagccca
     6241 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
     6301 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
     6361 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     6421 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     6481 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     6541 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     6601 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     6661 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     6721 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     6781 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acccgggcgg ggcgcgtctg
     6841 gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     6901 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     6961 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     7021 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     7081 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     7141 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     7201 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     7261 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     7321 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     7381 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat
     7441 taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct
     7501 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     7561 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     7621 ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca agatatcctt
     7681 gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg
     7741 ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca
     7801 gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg
     7861 catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca
     7921 tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct
     7981 tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg
     8041 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct
     8101 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     8161 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     8221 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     8281 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     8341 gaggccttga cattgctagc gtttaccgtc gacctctagc tagagcttgg cgtaatcatg
     8401 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     8461 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     8521 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     8581 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     8641 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     8701 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     8761 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     8821 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     8881 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     8941 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     9001 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     9061 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     9121 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     9181 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     9241 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     9301 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     9361 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     9421 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     9481 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     9541 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     9601 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     9661 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     9721 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     9781 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     9841 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     9901 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     9961 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    10021 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    10081 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    10141 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    10201 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    10261 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    10321 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    10381 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    10441 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    10501 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
    10561 cggatcggga gatcaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
    10621 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
    10681 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc
    10741 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct
    10801 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
    10861 ctacaaactt agtagt
//
