LOCUS       sequence-35220-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  TITLE     LIC N-terminal fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET His6 Gamma crystallin TEV LIC cloning vect...
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..44)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(110..130)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(683..700)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             719..741
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    756..780
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(780..799)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(781..799)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cgggctttgt tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tgcggccgca
       61 agcttgtcga cggcgctcga attcggatcc gttatccact tccaatattg gattggaagt
      121 acaggttttc ctcgatcccc tccacaatgc ggcggaaaga ctggacagct ggggaggctg
      181 caccccaatc gatgggcttc cggtactcct tcttgtccag gaggtactgc ctgccacggt
      241 agttgggtag ctcatagaaa atccagacac cctccagcac cttacaggag tggatctctc
      301 gcatgtgaaa ttgctccatg atggaagggc aatcttcggt ggtttcatac atctgaccac
      361 taaaatcccc tttctcaaag atctgaatct tatactggcc tccactaggc agatgaacag
      421 ctctgcagga gctgaggcgg tcgttgaggc ccatccaacg ctggtattca gggtactctc
      481 cctgtggtaa gatgtacatg tacccagcaa agttgggcct ttcataaaca gcccaggtgc
      541 ctccttccac tttaatggag ttgcagcgac ttaggtatgt gtggaaatct gcacagtcgc
      601 aatcacagtc atagcgacgg ccttgaaaat ttttgtcttc atagaaagta atcttggttc
      661 cagttttaga catagaagaa ccatggtgat ggtgatggtg agaagaaccc atggtatatc
      721 tccttcttaa agttaaacaa aattatttct agaggggaat tgttatccgc tcacaattcc
      781 cctatagtga gtcgtattaa tttcgcggga tcgagatctc gatcctctac gccgga
//
