LOCUS       sequence-352205-       10766 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry Vector compatible, fungal replicating, shuttle backbone vector
            from the Fungal Modular Cloning ToolKit..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10766)
  AUTHORS   Mozsik L, Pohl C, Meyer V, Bovenberg RAL, Nygard Y, Driessen AJM
  TITLE     Modular Synthetic Biology Toolkit for Filamentous Fungi.
  JOURNAL   ACS Synth Biol. 2021 Nov 2. doi: 10.1021/acssynbio.1c00260.
  PUBMED    34726388
REFERENCE   2  (bases 1 to 10766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2021 Nov 2. doi: 10.1021/acssynbio.1c00260."
COMMENT     Sequence Label: pFTK094-LM-AMA002.0
FEATURES             Location/Qualifiers
     source          1..10766
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(94..113)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(95..113)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(123..139)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      281..736
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(368..387)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     578..599
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        762..866
                     /gene="bla"
                     /label=AmpR promoter
     promoter        1186..1288
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1289..1948
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1355..1374)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2337..2925
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2826..2845
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3079..3096
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3213..3234
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3249..3254,3255..3272,3273..3279)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3287..3303
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3311..3327
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3346..3366
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3348..3366
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             4158..4532
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      4617..4864
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(4633..4651)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     rep_origin      4874..10122
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
     protein_bind    10231..10252
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10267..10272,10273..10290,10291..10297)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10305..10321
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10329..10345
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             10341..10664
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    10358..10414
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(10415..10431)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10640..10659)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gccgctcccc ccctgaaaga gttgatattg aaggtagtag gtacggtgca gggtaacgcg
       61 tgatggccgc caccgcggtg gagctccaat tcgccctata gtgagtcgta ttacgcgcgc
      121 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
      181 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
      241 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
      301 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
      361 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
      421 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
      481 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
      541 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
      601 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
      661 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
      721 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      781 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      841 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      901 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      961 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     1021 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttcgac
     1081 cgaataaata cctgtgacgg aagatcactt cgcagaataa ataaatcctg gtgtccctgt
     1141 tgataccggg aagccctggg ccaacttttg gcgaaaatga gacgttgatc ggcacgtaag
     1201 aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagttgtc
     1261 gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt
     1321 tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg
     1381 tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa
     1441 taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc
     1501 ggaattacgt atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg
     1561 ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga
     1621 cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct
     1681 ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt
     1741 gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt
     1801 caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt
     1861 tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta
     1921 ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc agttattggt gcccttaaac
     1981 gcctggttgc tacgcctgaa taagtgataa taagcggatg aatggcagaa attcgaaagc
     2041 aaattcgacc cggtcgtcgg ttcagggcag ggtcgttaaa tagccgctta tgtctattgc
     2101 tggtttaccg gtttattgac taccggaagc agtgtgaccg tgtgcttctc aaatgcctga
     2161 ggccagtttg ctcaggctct ccccgtggag gtaataattg acgatatgat cctttttttc
     2221 tgatcaaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     2281 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     2341 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     2401 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     2461 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     2521 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     2581 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     2641 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     2701 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     2761 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     2821 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2881 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2941 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     3001 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     3061 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     3121 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     3181 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     3241 ccccaggctt tacactttat gctcccggct cgtatgttgt gtggaattgt gagcggataa
     3301 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
     3361 taaagggaac aaaagctggg taccgggccc cccctcgagg tcgactacat gtatctgcat
     3421 gttgcatcgg gaaatcccac cacagggaca gccaagcggc cccgcgactt ggcagtgggc
     3481 aaactacgcc cgattctggt gccaagaacc gagaagaatg agacagaccc acgttgcact
     3541 ctaaccggat gctatcgact tacggtggct gaagattcaa cacgctgcaa cgagagccaa
     3601 ggtggtccgg acattttcta cgtgccggtt taccttggaa catcgccgtc gttgagtgca
     3661 cgttgcctac tctctcgtgg cttggctggg cccacgagcc cgattgactc gacggtgtta
     3721 cttgggtatc tatggccccg ttttctggca cggtaatgat aagtacttac tagtcttcga
     3781 gcgggggagt gttgctctgc ccgagcatca acgattggcc tgatcgcacc gtctgcaaat
     3841 gccacggtgc ggaccgactg aaatctcaga ccaccaaaga ccctccgact tcgagatacg
     3901 gttactaatt ttacactggc tccagcggcc ccatccagta agcatctggg ctgcaagcgt
     3961 ataatgtctc caggttgtct cagcataaac accccgcccc cgctcaggca cacaggaaga
     4021 gagctcaggt cgtttccatt gcgtccatac tcttcactca ttgtcatctg caggagaact
     4081 tcccctgtcc ctttgccaag ccctctcttc gtcgttgtcc acgccttcaa gttttcacca
     4141 ttatttttct agacaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg
     4201 acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg
     4261 aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg
     4321 accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt
     4381 acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga
     4441 ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact
     4501 gcgtgcactt cgtggccgag gagcaggact gaccgacgcc gaccaacacc gccggtccga
     4561 cgcggcccga cgggtccgag gcctcggaga tccgtccccc ttttcctttg tcgatatcat
     4621 gtaattagtt atgtcacgct tacattcacg ccctcccccc acatccgctc taaccgaaaa
     4681 ggaaggagtt agacaacctg aagtctaggt ccctatttat ttttttatag ttatgttagt
     4741 attaagaacg ttatttatat ttcaaatttt tctttttttt ctgtacagac gcgtgtacgc
     4801 atgtaacatt atactgaaaa ccttgcttga gaaggttttg ggacgctcga aggctttaat
     4861 ttgcaagctt gatagcttat tttttgtata ctgttttgtg atagcacgaa gtttttccac
     4921 ggtatcttgt taaaaatata tatttgtggc gggcttacct acatcaaatt aataagagac
     4981 taattataaa ctaaacacac aagcaagcta ctttagggta aaagtttata aatgcttttg
     5041 acgtataaac gttgcttgta tttattatta caattaaagg tggatagaaa acctagagac
     5101 tagttagaaa ctaatctcag gtttgcgtta aactaaatca gagcccgaga ggttaacaga
     5161 acctagaagg ggactagata tccgggtagg gaaacaaaaa aaaaaaacaa gacagccaca
     5221 tattagggag actagttaga agctagttcc aggactagga aaataaaaga caatgatacc
     5281 acagtctagt tgacaactag atagattcta gattgaggcc aaagtctctg agatccaggt
     5341 tagttgcaac taatactagt tagtatctag tctcctataa ctctgaagct agaataactt
     5401 actactatta tcctcaccac tgttcagctg cgcaaacgga gtgattgcaa ggtgttcaga
     5461 gactagttat tgactagtca gtgactagca ataactaaca aggtattaac ctaccatgtc
     5521 tgccatcacc ctgcacttcc tcgggctcag cagccttttc ctcctcattt tcatgctcat
     5581 tttccttgtt taagactgtg actagtcaaa gactagtcca gaaccacaaa ggagaaatgt
     5641 cttaccactt tcttcattgc ttgtctcttt tgcattatcc atgtctgcaa ctagttagag
     5701 tctagttagt gactagtccg acgaggactt gcttgtctcc ggattgttgg aggaactctc
     5761 cagggcctca agatccacaa cagagccttc tagaagactg gtcaataact agttggtctt
     5821 tgtctgagtc tgacttacga ggttgcatac tcgctccctt tgcctcgtca atcgatgaga
     5881 aaaagcgcca aaactcgcaa tatggctttg aaccacacgg tgctgagact agttagaatc
     5941 tagtcccaaa ctagcttgga tagcttacct ttgccctttg cgttgcgaca ggtcttgcag
     6001 ggtatggttc ctttctcacc agctgattta gctgccttgc taccctcacg gcggatctgc
     6061 ataaagagtg gctagaggtt ataaattagc actgatccta ggtacggggc tgaatgtaac
     6121 ttgcctttcc tttctcatcg cgcggcaaga caggcttgct caaattccta ccagtcacag
     6181 gggtatgcac ggcgtacgga ccacttgaac tagtcacaga ttagttagca actagtctgc
     6241 attgaatggc tgtacttacg ggccctcgcc attgtcctga tcatttccag cttcaccctc
     6301 gttgctgcaa agtagttagt gactagtcaa ggactagttg aaatgggaga agaaactcac
     6361 gaattctcga cacccttagt attgtggtcc ttggacttgg tgctgctata tattagctaa
     6421 tacactagtt agactcacag aaacttacgc agctcgcttg cgcttcttgg taggagtcgg
     6481 ggttgggaga acagtgcctt caaacaagcc ttcataccat gctacttgac tagtcaggga
     6541 ctagtcacca agtaatctag ataggacttg cctttggcct ccatcagttc cttcatagtg
     6601 ggaggtccat tgtgcaatgt aaactccatg ccgtgggagt tcttgtcctt caagtgcttg
     6661 accaatatgt ttctgttggc agagggaacc tgtcaactag ttaataacta gtcagaaact
     6721 agtatagcag tagactcact gtacgcttga ggcatccctt cactcggcag tagacttcat
     6781 atggatggat atcaggcacg ccattgtcgt cctgtggact agtcagtaac taggcttaaa
     6841 gctagtcggg tcggcttact atcttgaaat ccggcagcgt aagctccccg tccttaactg
     6901 cctcgagata gtgacagtac tctggggact ttcggagatc gttatcgcga atgctcggca
     6961 tactaatcgt tgactagtct tggactagtc ccgagcaaaa aggattggag gaggaggagg
     7021 aaggtgagag tgagacaaag agcgaaataa gagcttcaaa ggctatctct aagcagtatg
     7081 aaggttaagt atctagttct tgactagatt taaaagagat ttcgactagt tatgtacctg
     7141 gagtttggat ataggaatgt gttgtggtaa cgaaatgtaa gggggaggaa agaaaaagtc
     7201 ggtcaagagg taactctaag tcggccattc ctttttggga ggcgctaacc ataaacggca
     7261 tggtcgactt agagttagct cagggaattt agggagttat ctgcgaccac cgaggaacgg
     7321 cggaatgcca aagaatcccg atggagctct agctggcggt tgacaacccc accttttggc
     7381 gtttctgcgg cgttgcaggc gggactggat acttcgtaga accagaaagg caaggcagaa
     7441 cgcgctcagc aagagtgttg gaagtgatag catgatgtgc cttgttaact aggtcaaaat
     7501 ctgcagtatg cttgatgtta tccaaagtgt gagagaggaa ggtccaaaca tacacgattg
     7561 ggagagggcc taggtataag agtttttgag tagaacgcat gtgagcccag ccatctcgag
     7621 gagattaaac acgggccggc atttgatggc tatgttagta ccccaatgga aagcctgaga
     7681 gtccagtggt cgcagataac tccctaaatt ccctgagcta actctaagtc gaccatgccg
     7741 tttatggtta gcgcctccca aaaaggaatg gccgacttag agttacctct tgaccgactt
     7801 tttctttcct cccccttaca tttcgttacc acaacacatt cctatatcca aactccaggt
     7861 acataactag tcgaaatctc ttttaaatct agtcaagaac tagatactta accttcatac
     7921 tgcttagaga tagcctttga agctcttatt tcgctctttg tctcactctc accttcctcc
     7981 tcctcctcca atcctttttg ctcgggacta gtccaagact agtcaacgat tagtatgccg
     8041 agcattcgcg ataacgatct ccgaaagtcc ccagagtact gtcactatct cgaggcagtt
     8101 aaggacgggg agcttacgct gccggatttc aagatagtaa gccgacccga ctagctttaa
     8161 gcctagttac tgactagtcc acaggacgac aatggcgtgc ctgatatcca tccatatgaa
     8221 gtctactgcc gagtgaaggg atgcctcaag cgtacagtga gtctactgct atactagttt
     8281 ctgactagtt attaactagt tgacaggttc cctctgccaa cagaaacata ttggtcaagc
     8341 acttgaagga caagaactcc cacggcatgg agtttacatt gcacaatgga cctcccacta
     8401 tgaaggaact gatggaggcc aaaggcaagt cctatctaga ttacttggtg actagtccct
     8461 gactagtcaa gtagcatggt atgaaggctt gtttgaaggc actgttctcc caaccccgac
     8521 tcctaccaag aagcgcaagc gagctgcgta agtttctgtg agtctaacta gtgtattagc
     8581 taatatatag cagcaccaag tccaaggacc acaatactaa gggtgtcgag aattcgtgag
     8641 tttcttctcc catttcaact agtccttgac tagtcactaa ctactttgca gcaacgaggg
     8701 tgaagctgga aatgatcagg acaatggcga gggcccgtaa gtacagccat tcaatgcaga
     8761 ctagttgcta actaatctgt gactagttca agtggtccgt acgccgtgca tacccctgtg
     8821 actggtagga atttgagcaa gcctgtcttg ccgcgcgatg agaaaggaaa ggcaagttac
     8881 attcagcccc gtacctagga tcagtgctaa tttataacct ctagccactc tttatgcaga
     8941 tccgccgtga gggtagcaag gcagctaaat cagctggtga gaaaggaacc ataccctgca
     9001 agacctgtcg caacgcaaag ggcaaaggta agctatccaa gctagtttgg gactagattc
     9061 taactagtct cagcaccgtg tggttcaaag ccatattgcg agttttggcg ctttttctca
     9121 tcgattgacg aggcaaaggg agcgagtatg caacctcgta agtcagactc agacaaagac
     9181 caactagtta ttgaccagtc ttctagaagg ctctgttgtg gatcttgagg ccctggagag
     9241 ttcctccaac aatccggaga caagcaagtc ctcgtcggac tagtcactaa ctagactcta
     9301 actagttgca gacatggata atgcaaaaga gacaagcaat gaagaaagtg gtaagacatt
     9361 tctcctttgt ggttctggac tagtctttga ctagtcacag tcttaaacaa ggaaaatgag
     9421 catgaaaatg aggaggaaaa ggctgctgag cccgaggaag tgcagggtga tggcagacat
     9481 ggtaggttaa taccttgtta gttattgcta gtcactgact agtcaataac tagtctctga
     9541 acaccttgca atcactccgt ttgcgcagct gaacagtggt gaggataata gtagtaagtt
     9601 attctagctt cagagttata ggagactaga tactaactag tattagttgc aactaacctg
     9661 gatctcagag actttggcct caatctagaa tctatctagt tgtcaactag actgtggtat
     9721 cattgtcttt tattttccta gtcctggaac tagcttctaa ctagtctccc taatatgtgg
     9781 ctgtcttgtt tttttttttt gtttccctac ccggatatct agtccccttc taggttctgt
     9841 taacctctcg ggctctgatt tagtttaacg caaacctgag attagtttct aactagtctc
     9901 taggttttct atccaccttt aattgtaata ataaatacaa gcaacgttta tacgtcaaaa
     9961 gcatttataa acttttaccc taaagtagct tgcttgtgtg tttagtttat aattagtctc
    10021 ttattaattt gatgtaggta agcccgccac aaatatatat ttttaacaag ataccgtgga
    10081 aaaacttcgt gctatcacaa aacagtatac aaaaaataag ctatcgaatt cctgcagccc
    10141 cagatcatcc tgtcttcagt cttaaggagc gagacccagc tggcacgaca ggtttcccga
    10201 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
    10261 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
    10321 atttcacaca ggaaacagct atgaccatga ttacgccaag cttgcatgcc tgcaggtcga
    10381 ctctagagga tccccgggta ccgagctcga attcactggc cgtcgtttta caacgtcgtg
    10441 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
    10501 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
    10561 atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
    10621 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaaac cagccccgac
    10681 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
    10741 gacaagctgt ggtctcccgc tttaag
//
