LOCUS       sequence-352206-        3335 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-AA2
FEATURES             Location/Qualifiers
     source          1..3335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(55..73)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(78..94)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      401..989
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     890..909
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1116..1121,1122..1138,1139..1144)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             1192..2382
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1544..1563)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(2557..2575)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2580..2596)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat atcccctata
       61 gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc
      121 tctgatgtta cattgcacaa gataaaaata tatcatcatg cctcctctag accaaagctg
      181 gtctcgcagg ttgtagcgat tgctagtaat attggtggca aacaggctct tgagacggtt
      241 cagcgcctcc ttccagttct ctgtcaagcc cacggactca ccccagatca agtggttgag
      301 accatatctg gtcgagccac ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc
      361 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
      421 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
      481 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
      541 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
      601 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
      661 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
      721 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
      781 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
      841 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
      901 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
      961 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     1021 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatccaca gaatcagggg
     1081 ataacgcagg aaagaacatg aattaattct catgtttgac agcttatcat cgattagctt
     1141 taatgcggta gtttatcaca gttaaattgc taacgcagtc aggcaccgtg tatgaaatct
     1201 aacaatgcgc tcatcgtcat cctcggcacc gtcaccctgg atgctgtagg cataggcttg
     1261 gttatgccgg tactgccggg cctcttgcgg gatatcgtcc attccgacag catcgccagt
     1321 cactatggcg tgctgctagc gctatatgcg ttgatgcaat ttctatgcgc acccgttctc
     1381 ggagcactgt ccgaccgctt tggccgccgc ccagtcctgc tcgcttcgct acttggagcc
     1441 actatcgact acgcgatcat ggcgaccaca cccgtcctgt ggattctcta cgccggacgc
     1501 atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc
     1561 accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt
     1621 atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttaca tgcaccattc
     1681 cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag
     1741 tcgcataagg gagagcgccg acccatgccc ttgagagcct tcaacccagt cagctccttc
     1801 cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa
     1861 ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg
     1921 agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa
     1981 gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga agcaggccat tatcgccggc
     2041 atggcggccg acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc
     2101 ttccccatta tgattcttct cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg
     2161 ctgtccaggc aggtagatga cgaccatcag ggacagcttc aaggatcgct cgcggctctt
     2221 accagcctaa cttcgatcat tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg
     2281 agcacatgga acgggttggc atggattgta ggcgccgccc tataccttgt ctgcctcccc
     2341 gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct gaatggaagc cggcggcacc
     2401 tcgctaacgg attcaccact ccaagaattg gagccaatca attcttgcgg agaactgtga
     2461 atgcgcaaac caacccttga tcggggaaga acagtatgtc gagctatttt ttgacttact
     2521 ggggatcaag cctgattggg agaaaataaa atatccccta tagtgagtcg tattacatgg
     2581 tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac
     2641 aagataaaaa tatatcatca tgcctcctct agaccaaagc tggtctcgca ggttgtagcg
     2701 attgctagta atattggtgg caaacaggct cttgagacgg ttcagcgcct ccttccagtt
     2761 ctctgtcaag cccacggact caccccagat caagttgtag cgattgctag taatattggt
     2821 ggcaaacagg ctcttgagac ggttcagcgc ctccttccag ttctctgtca agcccacgga
     2881 ctcaccccag atcaagtggt tgagaccata tctggtcgag ccacccatga ccaaaatccc
     2941 ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     3001 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     3061 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3121 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     3181 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3241 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3301 ataaggcgca gcggtcgggc tgaacggggg gttcg
//
