LOCUS       sequence-352207-        2602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-AA3
FEATURES             Location/Qualifiers
     source          1..2602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      368..956
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     857..876
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1083..1088,1089..1105,1106..1111)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             1159..2349
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1511..1530)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2523..2542)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2524..2542)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2547..2563)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2547..2563)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tgcacaagat aaaaatatat catcatgcct cctctagacc aaagctggtc tcgtggtagc
       61 gattgctagt aatattggtg gcaaacaggc tcttgagacg gttcagcgcc tccttccagt
      121 tctctgtcaa gcccacggac tcaccccaga tcaagttgta gcgattgcta gtaatattgg
      181 tggcaaacag gctcttgaga cggttcagcg cctccttcca gttctctgtc aagcccacgg
      241 actcacccca gatcaagttg tagtgagacc atatctggtc gagccaccca tgaccaaaat
      301 cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
      361 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
      421 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
      481 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
      541 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
      601 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
      661 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
      721 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
      781 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
      841 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
      901 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
      961 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     1021 ttcctgcgtt atccacagaa tcaggggata acgcaggaaa gaacatgaat taattctcat
     1081 gtttgacagc ttatcatcga ttagctttaa tgcggtagtt tatcacagtt aaattgctaa
     1141 cgcagtcagg caccgtgtat gaaatctaac aatgcgctca tcgtcatcct cggcaccgtc
     1201 accctggatg ctgtaggcat aggcttggtt atgccggtac tgccgggcct cttgcgggat
     1261 atcgtccatt ccgacagcat cgccagtcac tatggcgtgc tgctagcgct atatgcgttg
     1321 atgcaatttc tatgcgcacc cgttctcgga gcactgtccg accgctttgg ccgccgccca
     1381 gtcctgctcg cttcgctact tggagccact atcgactacg cgatcatggc gaccacaccc
     1441 gtcctgtgga ttctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg
     1501 gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg
     1561 ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg
     1621 ggcgccatct ccttacatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta
     1681 ctactgggct gcttcctaat gcaggagtcg cataagggag agcgccgacc catgcccttg
     1741 agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca
     1801 cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc
     1861 attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta
     1921 ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc
     1981 ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta cgtcttgctg
     2041 gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc ttccggcggc
     2101 atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga ccatcaggga
     2161 cagcttcaag gatcgctcgc ggctcttacc agcctaactt cgatcattgg accgctgatc
     2221 gtcacggcga tttatgccgc ctcggcgagc acatggaacg ggttggcatg gattgtaggc
     2281 gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag ccgggccacc
     2341 tcgacctgaa tggaagccgg cggcacctcg ctaacggatt caccactcca agaattggag
     2401 ccaatcaatt cttgcggaga actgtgaatg cgcaaaccaa cccttgatcg gggaagaaca
     2461 gtatgtcgag ctattttttg acttactggg gatcaagcct gattgggaga aaataaaata
     2521 tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt
     2581 ctcaaaatct ctgatgttac at
//
