LOCUS       sequence-35224-e         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  TITLE     2-series LIC N-terminal fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET LIC cloning vector (2A-T)
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             160..182
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(310..328)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      324..371
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(737..742,743..759,760..765))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga
       61 ctcactatag ggagaccaca acggtttccc tctagtgccg gctccggaga gctctttaat
      121 taagcggccg ccctgcagga ctcgagttct agaaataatt ttgtttaact ttaagaagga
      181 gatatagata tcccaactcc ataaggatcc gcgatcgcgg cgcgccacct ggtggccggc
      241 cggtaccacg cgtgcgcgct gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
      301 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
      361 ggggtttttt gctgaaagga ggaactatat ccggacatcc acaggacggg tgtggtcgcc
      421 atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca
      481 aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc
      541 gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgacatc ccgcaagagg
      601 cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg
      661 atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac
      721 tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg
      781 a
//
