LOCUS       sequence-352243-        5941 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Sherley JL
  TITLE     Guanine nucleotide biosynthesis is regulated by the cellular p53 
            concentration.
  JOURNAL   J Biol Chem. 1991 Dec 25. 266(36):24815-28.
  PUBMED    1761576
REFERENCE   2  (bases 1 to 5941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1991 Dec 25. 266(36):24815-28."
COMMENT     Sequence Label: pJS1a (tsE2)
FEATURES             Location/Qualifiers
     source          1..5941
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(59..79)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        64..393
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      244..379
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          432..528
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             2433..3227
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2487..2506)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3097..3116
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3409..3543
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3433..3452)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3487..3506
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3686..3708)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3794..3934
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3849..3868
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3949..3966)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4120..4708)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4200..4219)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4879..5670,5671..5739))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5502..5521
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5740..5844)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5912..5930
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 caagaattgg tcgaccaatt ctcatgtttg acagcttatc atcgataagc ttggctgtgg
       61 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
      121 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
      181 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
      241 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
      301 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
      361 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc tcgaggaact gaaaaaccag
      421 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      481 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      541 gtgttacttc tgctctaaaa gctgctgcag gggggggggg gagaaaacac aaagcagcgg
      601 ttcaaattaa agctccaccc ctcctggtaa ccagtaatat tgatgtgcag gcagaggaca
      661 gatatttgta cttgcatagt cgggtgcaaa cctttcgctt tgagcagcca tgcacagatg
      721 aatcgggtga gcaacctttt aatattactg atgcagattg gaaatctttt tttgtaaggt
      781 tatgggggcg tttagacctg attgacgagg aggaggatag tgaagaggat ggagacagca
      841 tgcgaacgtt tacatgtagc gcaagaaaca caaatgcagt tgattgagaa aagtagtgat
      901 aagttgcaag atcatatact gtactggact gctgttagaa ctgagaacac actgctttat
      961 gctgcaagga aaaaaggggt gactgtccta ggacactgca gagtaccaca ctctgtagtt
     1021 tgtcaagaga gagccaagca ggccattgaa atgcagttgt ctttgcagga gttaagcaaa
     1081 actgagtttg gggatgaacc atggtctttg cttgacacaa gctgggaccg atatatgtca
     1141 gaacctaaac ggtgctttaa gaaaggcgcc agggtggtag aggtggagtt tgatggaaat
     1201 gcaagcaata caaactggta cactgtctac agcaatttgt acatgcgcac agaggacggc
     1261 tggcagcttg cgaaggctgg ggctgacgga actgggctct actactgcac catggccggt
     1321 gctggacgca tttactattc tcgctttggt gacgaggcag ccagatttag tcctcgatcg
     1381 aggacaacag ggcattactc tgtaagagat caggacagag tgtatgctgg tgtctcatcc
     1441 acctcttctg attttagaga tcgcccagac ggagtctggg tcgcatccga aggacctgaa
     1501 ggagaccctg caggaaaaga agccgagcca gcccagcctg tctcttcttt gctcggctcc
     1561 cccgcctgcg gtcccatcag agcaggcctc ggttgggtac gggacggtcc tcgctcgcac
     1621 ccctacaatt ttcctgcagg ctcggggggc tctattctcc gctcttcctc caccccggtg
     1681 cagggcacgg taccggtgga cttggcatca aggcaggaag aagaggagca gtcgcccgac
     1741 tccacagagg aagaaccagt gactctccca aggcgcacca ccaatgatgg attccacctg
     1801 ttaaaggcag gagggtcatg ctttgctcta atttcaggaa ctgctaacca ggtaaagtgc
     1861 tatcgctttc gggtgaaaaa gaaccataga catcgctacg agaactgcac caccacctgg
     1921 ttcacagttg ctgacaacgg tgctgaaaga caaggacaag cacaaatact gatcaccttt
     1981 ggatcgccaa gtcaaaggca agactttctg aaacatgtac cactacctcc tggaatgaac
     2041 atttccggct ttacagccag cttggacttc tgatccgagc ttcacgctgc cgcaagcact
     2101 cagggcgcaa gggctgctaa aggaagcgga acacgtagaa agccagtccg cagaaacggt
     2161 gctgaccccg gatgaatgtc agctactggg ctatctggac aagggaaaac gcaagcgcaa
     2221 agagaaagca ggtagcttgc agtgggctta catggcgata gctagactgg gcggttttat
     2281 ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt gggaagccct
     2341 gcaaagtaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg ggatcaagat
     2401 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     2461 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     2521 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     2581 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     2641 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     2701 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     2761 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     2821 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     2881 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     2941 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     3001 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     3061 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     3121 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     3181 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     3241 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     3301 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     3361 ccagcgcggg gatctcatgc tggagttctt cgcccacccc ggaattcgga tccagacatg
     3421 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     3481 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     3541 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     3601 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatcc ggctgcctcg
     3661 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     3721 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     3781 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct
     3841 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     3901 gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga
     3961 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     4021 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     4081 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     4141 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     4201 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     4261 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     4321 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     4381 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     4441 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     4501 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     4561 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     4621 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     4681 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     4741 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     4801 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     4861 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     4921 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     4981 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     5041 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     5101 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     5161 agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc
     5221 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     5281 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     5341 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     5401 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     5461 tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag
     5521 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     5581 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     5641 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     5701 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     5761 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     5821 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     5881 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     5941 t
//
