LOCUS       sequence-352246-       11135 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of mouse Dnajc16-V5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11135)
  AUTHORS   Cheng LC, Zhang X, Baboo S, Nguyen JA, Martinez-Bartolome S, Loose 
            E, Diedrich J, Yates JR 3rd, Gerace L
  TITLE     Comparative membrane proteomics reveals diverse cell regulators 
            concentrated at the nuclear envelope.
  JOURNAL   Life Sci Alliance. 2023 Jul 11;6(9):e202301998. doi: 
            10.26508/lsa.202301998. Print 2023 Sep.
  PUBMED    37433644
REFERENCE   2  (bases 1 to 11135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2023-07-11"; volume: "6(9):e202301998. doi"; 
            pages: " 10.26508/lsa.202301998. Print 2023 Sep"
COMMENT     Sequence Label: pLV Dnajc16-V5
FEATURES             Location/Qualifiers
     source          1..11135
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2221..2432
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2433..3371
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3328..3348
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3402..3411
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5751..5792
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5929..6478
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6073..6090)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6300..6319
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6481..7080
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6481..6500)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6977..6997
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7104..7692
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7157..7177)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7575..7586)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7899..8532
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8660..8676)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8660..8676)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8673..8695)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8684..8700
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8708..8714,8715..8732,8733..8738))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8753..8774
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8891..8908)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9062..9650)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9142..9161)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9821..10612,10613..10681))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10444..10463
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10682..10786)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10834..10968
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10871..10890)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10925..10944
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatccgcca tcggcccgcc cgccgccatg gaattgaaaa
     3421 ggttgggcgt gtcctggcga ttcttgatgg ttctggttct gatcctgcaa agcctgtctg
     3481 cattggattt tgacccatac agagtcctag gggtcagccg cacggccagt caggctgaca
     3541 ttaaaaaggc atataagaag ctcgctcggg aatggcatcc tgacaaaaac aaagaccctg
     3601 gagccgagga caggttcatt cagatcagca aggcatacga gatcctgtcc aatgaggaaa
     3661 agaggacaaa ctacgaccac tatggtgatg ccggtgagaa ccaaggctac cagaagcagc
     3721 agcgcgaaca ccgcttccgc catttccacg agaacttcta ttttgatgag tcctttttcc
     3781 acttcccctt caatgcggag cggcgggact cgggtgacga gaagtacttg ctgcacttct
     3841 cacactacgt gaatgaagtg cttcccgaga gcttcaaaag accctacctc atcaagatca
     3901 cgtcagactg gtgcttcagc tgcatccata tcgagcctgt ctggaaggag gtggtccagg
     3961 aactggaagg actgggtgtg gggattggtg tcgtccacgc tgggtacgaa aggcgcctgg
     4021 cccatcacct gggagcacac agcaccccct ccatccttgg ggtcatcagt gggaagatca
     4081 ccttcttcca caacgcagtg gtccatgaaa acctacggca gttcgtggag agtcttctcc
     4141 caggaaactt ggtagagaag gtcacaaata aaaattatgt gcggttcctc tctggctggc
     4201 agcaggagaa taagccacac gccctcctgt tcggccagac accagcagtg ccactgatgt
     4261 acaagttgac ggccttcgca tacaaggatt atgtgtcgtt tggatacgtg tatgtcggct
     4321 tgagaggtgt ggaagagatg accaggcagt acaacgtcaa cctctatacc cccaccatgc
     4381 tcatcttcaa agagcacata aacaagcccg ctgatgtgat ccaggctcgg ggactgaaga
     4441 agcaggtcat cgaggacttc atcgcccaaa acaagtacct gctggcctcc cggctcacca
     4501 gccagaggtt gttccatgaa ctctgccctg tgaagcggtc tcaccgacag agaaaatact
     4561 gcgtggtttt actgacagct gagactaaca aagtgagcaa accctttgaa gccttcctgt
     4621 catttgccct ggcaaacact caagacacgg tacggttcgt gcatgtctac agcaaccgac
     4681 agcaggagtt tgccagcaca ttgctaccgg acatggaagc tttccagggg aagtcagggg
     4741 tgtccatctt ggaaaggagg aacacagcgg ggagggtggt gttcaaaacc ctggaggatc
     4801 cctggaccgg cagtgagagc gataagttcg tccttttggg ttatcttgac cagctgcgga
     4861 aggatccagc atttctgtct tcagaagctg tgctccctga cctgactgac gagctggctc
     4921 ctgtattttt cctccggtgg ctctactctg tttctgacta cctctcggac ttctgggaga
     4981 gcctgcttca cagtaactgg cgggagatga tgcccctgct gtccctcatc ttctcggccc
     5041 tcttcatcct cttcggcacg gtcatggtcc aggctttcag tgactcaaat gaggagcgag
     5101 agtcgcaccc tgcagacaaa gaggaagtcc ccgagaaggc tgggaagact gagccgagct
     5161 tcaccaaaga gagcagcagc aagattccta agaaaggctt tgtggaggtg actgaactca
     5221 cagatgtgac gtacaccagc aatttggtac gcctgaggcc tggtcacatg aatgtggtcc
     5281 tgatcctgtc caattccact aagacaagcc tcctgcagaa gtttgctttg gaagtctaca
     5341 cattcaccgg gagcagcagc ctccacttct ccttcctgac actggacaaa caccgggagt
     5401 ggctggagta tttgctagag tttgctcagg atgcggcccc gatcccaaac cagtatgaca
     5461 agcactttat ggaacgagac tacactggct acgtgctggc cctgaacggt cacaagaagt
     5521 atttctgcct tttcaagcca ctaaagacag tggatgagga gactgtggcc tcctgtgacc
     5581 ctgactcctc ccgagggaag ccttcctgtg gccttggccc caagcccctc aaaggcaagc
     5641 tgagcaagtt gtccttgtgg atggagcgcc tgctggaagg ctccttgcag aggttctaca
     5701 tcccgtcgtg gcctgagctg gacggtgggc cgggcggggg tggcggggga ggtaagccta
     5761 tccctaaccc tctcctcggt ctcgattcta cgtgagttta aacttcgaaa cgcgtgaatt
     5821 cctcgagggc ggccgctcta gagtcgacgg gccgcggtaa caattgttaa ctaacttaag
     5881 ctagcaacgg tttccctcta gcgggatcaa ttccgccccc cccccctaac gttactggcc
     5941 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc
     6001 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta
     6061 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag
     6121 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
     6181 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg
     6241 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat
     6301 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta
     6361 tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa
     6421 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgataatacc
     6481 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     6541 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     6601 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     6661 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     6721 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     6781 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     6841 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     6901 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     6961 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     7021 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     7081 cccgggcggg gcgcgtctgg aacaatcaac ctctggatta caaaatttgt gaaagattga
     7141 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     7201 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     7261 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     7321 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     7381 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     7441 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
     7501 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     7561 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     7621 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     7681 ccgcctcccc gcctggaatt aattctgcag tcgagaccta gaaaaacatg gagcaatcac
     7741 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga
     7801 ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc
     7861 tgtagatctt agccactttt taaaagaaaa gaggggactg gaagggctaa ttcactccca
     7921 acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta
     7981 gcagaactac acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa
     8041 gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt
     8101 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag
     8161 gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa
     8221 gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg
     8281 tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt
     8341 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     8401 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     8461 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     8521 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     8581 aatgaatatc agagagtgag aggccttgac attgctagcg tttaccgtcg acctctagct
     8641 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     8701 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     8761 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     8821 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     8881 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     8941 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     9001 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     9061 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     9121 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     9181 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     9241 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     9301 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     9361 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     9421 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     9481 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     9541 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     9601 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     9661 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     9721 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     9781 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     9841 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     9901 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     9961 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
    10021 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
    10081 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
    10141 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    10201 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    10261 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    10321 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    10381 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    10441 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    10501 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    10561 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    10621 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    10681 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    10741 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    10801 aagtgccacc tgacgtcgac ggatcgggag atcaacttgt ttattgcagc ttataatggt
    10861 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
    10921 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact
    10981 caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac
    11041 ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat
    11101 tgtatttgtt aaatatgtac tacaaactta gtagt
//
