LOCUS       sequence-35226-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  TITLE     2-series LIC N-terminal fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET His6 MBP N10 TEV LIC cloning vector (2C-T)
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     58..77
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        58..76
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             165..187
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             207..224
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaattaa
       61 tacgactcac tatagggaga ccacaacggt ttccctctag tgccggctcc ggagagctct
      121 ttaattaagc ggccgccctg caggactcga gttctagaaa taattttgtt taactttaag
      181 aaggagatat acatatgaaa tcttctcacc atcaccatca ccatggttct tctatgaaaa
      241 tcgaagaagg taaactggta atctggatta acggcgataa aggctataac ggtctcgctg
      301 aagtcggtaa gaaattcgag aaagataccg gaattaaagt caccgttgag catccggata
      361 aactggaaga gaaattccca caggttgcgg caactggcga tggccctgac attatcttct
      421 gggcacacga ccgctttggt ggctacgctc aatctggcct gttggctgaa atcaccccgg
      481 acaaagcgtt ccaggacaag ctgtatccgt ttacctggga tgccgtacgt tacaacggca
      541 agctgattgc ttacccgatc gctgttgaag cgttatcgct gatttataac aaagatctgc
      601 tgccgaaccc gccaaaaacc tgggaagaga tcccggcgct ggataaagaa ctgaaagcga
      661 aaggtaagag cgcgctgatg ttcaacctgc aagaaccgta cttcacctgg ccgctgattg
      721 ctgctgacgg gggttatgcg ttcaagtatg aaaacggcaa gtacgacatt aaagacgtgg
      781 gcgtggataa cgctggcgcg aaagcgggtc tgaccttcct ggttgacctg attaaaaaca
      841 aacac
//
