LOCUS       sequence-35228-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  TITLE     2-series LIC N-terminal fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET His6 MBP TEV LIC cloning vector (2M-T)
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             162..184
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             204..221
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaattaatac
       61 gactcactat agggagacca caacggtttc cctctagtgc cggctccgga gagctcttta
      121 attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa ctttaagaag
      181 gagatataca tatgaaatct tctcaccatc accatcacca tggttcttct atgaaaatcg
      241 aagaaggtaa actggtaatc tggattaacg gcgataaagg ctataacggt ctcgctgaag
      301 tcggtaagaa attcgagaaa gataccggaa ttaaagtcac cgttgagcat ccggataaac
      361 tggaagagaa attcccacag gttgcggcaa ctggcgatgg ccctgacatt atcttctggg
      421 cacacgaccg ctttggtggc tacgctcaat ctggcctgtt ggctgaaatc accccggaca
      481 aagcgttcca ggacaagctg tatccgttta cctgggatgc cgtacgttac aacggcaagc
      541 tgattgctta cccgatcgct gttgaagcgt tatcgctgat ttataacaaa gatctgctgc
      601 cgaacccgcc aaaaacctgg gaagagatcc cggcgctgga taaagaactg aaagcgaaag
      661 gtaagagcgc gctgatgttc aacctgcaag aaccgtactt cacctggccg ctgattgctg
      721 ctgacggggg ttatgcgttc aagtatgaaa acggcaagta cgacattaaa gacgtgggcg
      781 tggataacgc tggcgcgaaa gcgggtctga ccttcctggt tgacctgatt aaaaacaaa
//
