LOCUS       sequence-35229-e         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  TITLE     2-series LIC N-terminal fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET His6 NusA TEV LIC cloning vector (2N-T)
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     58..77
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        58..76
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             165..187
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             207..224
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gtgatgccgg caacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaattaa
       61 tacgactcac tatagggaga ccacaacggt ttccctctag tgccggctcc ggagagctct
      121 ttaattaagc ggccgccctg caggactcga gttctagaaa taattttgtt taactttaag
      181 aaggagatat acatatgaaa tcttctcacc atcaccatca ccatggttct tctatgaaca
      241 aagaaatttt ggctgtagtt gaagccgtat ccaatgaaaa ggcgctacct cgcgagaaga
      301 ttttcgaagc attggaaagc gcgctggcga cagcaacaaa gaaaaaatat gaacaagaga
      361 tcgacgtccg cgtacagatc gatcgcaaaa gcggtgattt tgacactttc cgtcgctggt
      421 tagttgttga tgaagtcacc cagccgacca aggaaatcac ccttgaagcc gcacgttatg
      481 aagatgaaag cctgaacctg ggcgattacg ttgaagatca gattgagtct gttacctttg
      541 accgtatcac tacccagacg gcaaaacagg ttatcgtgca gaaagtgcgt gaagccgaac
      601 gtgcgatggt ggttgatcag ttccgtgaac acgaaggtga aatcatcacc ggcgtggtga
      661 aaaaagtaaa ccgcgacaac atctctctgg atctgggcaa caacgctgaa gccgtgatcc
      721 tgcgcgaaga tatgctgccg cgtgaaaact tccgccctgg cgaccgcgtt cgtggcgtgc
      781 tctattccgt tcgcccggaa gcgcgtggcg cgcaac
//
