LOCUS       sequence-35230-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  TITLE     2-series LIC N-terminal fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET His6 Mocr TEV LIC cloning vector (2O-T)
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             162..184
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             204..221
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             591..611
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(736..754)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      750..797
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaattaatac
       61 gactcactat agggagacca caacggtttc cctctagtgc cggctccgga gagctcttta
      121 attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa ctttaagaag
      181 gagatataca tatgaaatct tctcaccatc accatcacca tggttcttct atgagcaaca
      241 tgacatacaa taatgttttc gaccacgcgt acgaaatgct taaggagaac atccgttatg
      301 atgacatacg ggatacagat gaccttcacg acgcaattca tatggccgcc gataatgccg
      361 tccctcacta ttatgccgat attcggtcag ttatggcatc tgaaggtatt gatctggaat
      421 ttgaggatag tggcctcatg ccagatacga aggacgacat cagaattctg caagcgcgga
      481 tctacgaaca acttaccatc gatttatggg aagatgcgga agatctcctg aatgaatacc
      541 tggaagaagt ggaggagtat gaggaagatg aagaggggac ggggatcgag gaaaacctgt
      601 acttccaatc caatattgga agtggataac ggatccgcga tcgcggcgcg ccacctggtg
      661 gccggccggt accacgcgtg cgcgctgatc cggctgctaa caaagcccga aaggaagctg
      721 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
      781 tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg
      841 gtcgccatg
//
