LOCUS       sequence-35233-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  TITLE     2-series LIC N-terminal fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET His6 ProteinG TEV LIC cloning vector (2P-T)
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             157..179
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             199..216
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             235..396
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     CDS             406..426
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(551..569)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      565..612
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt aatacgactc
       61 actataggga gaccacaacg gtttccctct agtgccggct ccggagagct ctttaattaa
      121 gcggccgccc tgcaggactc gagttctaga aataattttg tttaacttta agaaggagat
      181 atacatatga aatcttctca ccatcaccat caccatggtt cttctatgac tacttacaaa
      241 ttaatcctta atggtaaaac attgaaaggc gaaacaacta ctgaagctgt tgatgctgct
      301 actgcagaaa aagtcttcaa acaatacgct aacgacaacg gtgttgacgg tgaatggact
      361 tacgacgatg cgactaagac ctttacagtt actgaaggga tcgaggaaaa cctgtacttc
      421 caatccaata ttggaagtgg ataacggatc cgcgatcgcg gcgcgccacc tggtggccgg
      481 ccggtaccac gcgtgcgcgc tgatccggct gctaacaaag cccgaaagga agctgagttg
      541 gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
      601 aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc
      661 catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc
      721 aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag
      781 cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgc
//
