LOCUS       sequence-35234-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  TITLE     2-series LIC N-terminal fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET His6 Gamma crystallin TEV LIC cloning vect...
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             162..184
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             204..221
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             774..794
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaattaatac
       61 gactcactat agggagacca caacggtttc cctctagtgc cggctccgga gagctcttta
      121 attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa ctttaagaag
      181 gagatataca tatgaaatct tctcaccatc accatcacca tggttcttct atgtctaaaa
      241 ctggaaccaa gattactttc tatgaagaca aaaattttca aggccgtcgc tatgactgtg
      301 attgcgactg tgcagatttc cacacatacc taagtcgctg caactccatt aaagtggaag
      361 gaggcacctg ggctgtttat gaaaggccca actttgctgg gtacatgtac atcttaccac
      421 agggagagta ccctgaatac cagcgttgga tgggcctcaa cgaccgcctc agctcctgca
      481 gagctgttca tctgcctagt ggaggccagt ataagattca gatctttgag aaaggggatt
      541 ttagtggtca gatgtatgaa accaccgaag attgcccttc catcatggag caatttcaca
      601 tgcgagagat ccactcctgt aaggtgctgg agggtgtctg gattttctat gagctaccca
      661 actaccgtgg caggcagtac ctcctggaca agaaggagta ccggaagccc atcgattggg
      721 gtgcagcctc cccagctgtc cagtctttcc gccgcattgt ggaggggatc gaggaaaacc
      781 tgtacttcca atccaatatt ggaagtggat aacggatccg cgatcgcggc gcgccacc
//
