LOCUS       sequence-35237-e         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  TITLE     2-series LIC N-terminal fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET StrepII TEV LIC cloning vector (2R-T)
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             160..182
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             199..222
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             229..249
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(374..392)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      388..435
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga
       61 ctcactatag ggagaccaca acggtttccc tctagtgccg gctccggaga gctctttaat
      121 taagcggccg ccctgcagga ctcgagttct agaaataatt ttgtttaact ttaagaagga
      181 gatatacata tggctagctg gagccacccg cagttcgaaa aaggcgccga aaacctgtac
      241 ttccaatcca atattggaag tggataacgg atccgcgatc gcggcgcgcc acctggtggc
      301 cggccggtac cacgcgtgcg cgctgatccg gctgctaaca aagcccgaaa ggaagctgag
      361 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
      421 ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt
      481 cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg
      541 gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata
      601 tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa
      661 gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc
      721 gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt
      781 taactg
//
