LOCUS       sequence-35238-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  TITLE     2-series LIC N-terminal fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET His6 StrepII TEV LIC cloning vector (2HR-T)
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             160..182
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             202..219
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             235..258
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             265..285
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(410..428)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      424..471
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(837..842,843..859,860..865))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga
       61 ctcactatag ggagaccaca acggtttccc tctagtgccg gctccggaga gctctttaat
      121 taagcggccg ccctgcagga ctcgagttct agaaataatt ttgtttaact ttaagaagga
      181 gatatacata tgaaatcttc tcaccatcac catcaccatg gttcttctgc tagctggagc
      241 cacccgcagt tcgaaaaagg cgccgaaaac ctgtacttcc aatccaatat tggaagtgga
      301 taacggatcc gcgatcgcgg cgcgccacct ggtggccggc cggtaccacg cgtgcgcgct
      361 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
      421 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga
      481 ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt
      541 ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc
      601 gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg
      661 actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac
      721 caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt
      781 agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta
      841 aagcttatcg atgataagct gtcaaacatg agaatt
//
