LOCUS       sequence-352383-       10964 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of human DDX1-V5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10964)
  AUTHORS   Hammond JA, Zhou L, Lamichhane R, Chu HY, Millar DP, Gerace L, 
            Williamson JR
  TITLE     A Survey of DDX21 Activity During Rev/RRE Complex Formation.
  JOURNAL   J Mol Biol. 2018 Feb 16;430(4):537-553. doi: 
            10.1016/j.jmb.2017.06.023. Epub 2017 Jul 10.
  PUBMED    28705764
REFERENCE   2  (bases 1 to 10964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2017.06.023"; journalName: "J Mol Biol"; date: 
            "2018-02-16- 16"; volume: "430"; issue: "4"; pages: "537-553"
COMMENT     Sequence Label: pLV CMV mCherry-DDX1-V5
FEATURES             Location/Qualifiers
     source          1..10964
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(131..199,200..991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(349..368)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1162..1750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1651..1670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1904..1921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2038..2059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2074..2079,2080..2097,2098..2104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2112..2128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2117..2139
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2136..2152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2136..2152
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2171..2191
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2173..2191
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2219..2445
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2446..2626
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2673..2798
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3291..3524
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3709..3753
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4020..4136
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4159..4462
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4463..4666
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4617..4637
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4663..4687
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4762..5469
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4907..4925)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5175..5194
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             7756..7797
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7807..7824
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    7842..8430
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7895..7915)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8313..8324)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        8444..8943
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8479..8502)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8876..8895
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8964..9563
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8964..8983)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9460..9480
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             9701..9934
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10006..10127
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10043..10062)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10097..10116
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10167..10302
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10322..10341)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10323..10341)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10351..10368)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10351..10367)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10360..10382)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10509..10937
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10596..10615)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10806..10827
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
       61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
      601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
      661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
      721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
      781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
      841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
      901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
      961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     2401 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     2461 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     2521 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     2581 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     2641 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     2701 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     2761 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     2821 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     2881 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     2941 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     3001 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     3061 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     3121 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     3181 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     3241 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     3301 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     3361 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     3421 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     3481 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     3541 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     3601 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     3661 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     3721 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     3781 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     3841 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     3901 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     3961 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
     4021 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     4081 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg
     4141 ataagcttgg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     4201 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     4261 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     4321 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     4381 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     4441 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     4501 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     4561 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     4621 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
     4681 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgactcta
     4741 gaggatccac cggtgccaac catggtgagc aagggcgagg aggataacat ggccatcatc
     4801 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
     4861 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     4921 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
     4981 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
     5041 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
     5101 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
     5161 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
     5221 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
     5281 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
     5341 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
     5401 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
     5461 ctgtacaagg gcggaggtgg gtcgggtggc ggcggatcag ctagcatggc ggccttctcc
     5521 gagatgggtg taatgcctga gattgcacaa gctgtggaag agatggattg gctcctccca
     5581 actgatatcc aggctgaatc tatcccattg atcttaggag gaggtgatgt acttatggct
     5641 gcagaaacag gaagtggcaa aactggtgct tttagtattc cagttatcca gatagtttat
     5701 gaaactctga aagaccaaca ggaaggcaaa aaaggaaaaa caacaattaa aactggtgct
     5761 tcagtgctga acaaatggca gatgaaccca tatgacagag gatctgcttt tgcaattggg
     5821 tcagatggtc tttgttgtca aagcagagaa gtaaaggaat ggcatgggtg tagagctact
     5881 aaaggattaa tgaaagggaa acactactat gaagtatcct gtcatgacca agggttatgc
     5941 agggtcgggt ggtctaccat gcaggcctct ttggacctag gtactgacaa gtttggattt
     6001 ggctttggtg gaacaggaaa gaaatcccat aacaaacaat ttgataatta tggagaggaa
     6061 ttcactatgc atgataccat tggatgttac ctggatatag ataagggaca tgtcaagttc
     6121 tccaaaaatg gaaaagatct tggtctggca tttgaaatac caccacatat gaaaaaccaa
     6181 gccctctttc ctgcctgtgt tttgaagaat gctgaactga aatttaactt cggtgaagag
     6241 gaatttaagt ttccaccaaa agatggcttt gttgctcttt ccaaggcacc ggatggttac
     6301 attgtcaaat cacagcactc aggtaatgca caggtgacac aaacaaagtt tctccccaat
     6361 gctccgaaag ctctcattgt tgaaccttcc cgggagttag ctgaacaaac tttgaacaac
     6421 atcaagcagt ttaagaaata cattgataat cctaaattaa gggagcttct gataattgga
     6481 ggtgttgcag cccgggatca gctctctgtt ttggaaaatg gagtagatat agttgtaggt
     6541 actccgggaa gactagatga cttggtgtca actggaaagc tgaacttatc tcaagttaga
     6601 ttcctggtcc tggatgaagc tgatgggctt ctttctcaag gttattctga ttttataaat
     6661 aggatgcaca atcagattcc tcaggttacc tctgatggaa aaagacttca ggtgattgtt
     6721 tgctctgcca ctttgcattc tttcgatgta aagaaactgt ccgagaagat aatgcatttt
     6781 cctacatggg ttgacttaaa aggagaagac tctgttccag atactgtaca ccatgttgtt
     6841 gtcccagtaa atcccaaaac tgacagactc tgggaaaggc ttggaaagag ccacattaga
     6901 actgatgatg tacatgcaaa agataacaca agacctggtg ctaatagtcc agagatgtgg
     6961 tctgaagcta ttaaaatcct gaaaggggag tatgctgtcc gggcaatcaa ggaacataag
     7021 atggatcaag caattatctt ctgtagaacc aaaattgact gtgataactt ggagcagtac
     7081 tttatacaac aaggaggagg acctgataaa aaaggacacc agttctcatg tgtttgtctt
     7141 catggtgaca gaaagcctca tgagagaaag caaaacttgg aaagatttaa gaaaggagat
     7201 gtaagattct tgatttgcac agatgtagct gctagaggaa ttgatatcca cggtgttcct
     7261 tatgttataa atgtcactct gcccgatgaa aagcaaaact acgtacatcg aattggcaga
     7321 gtaggaagag ctgaaaggat gggtctggca atttccctgg tggcaacaga aaaagaaaag
     7381 gtttggtacc atgtatgtag cagccgtgga aaagggtgtt ataacacaag actcaaggaa
     7441 gatggaggct gtaccatatg gtacaacgag atgcagttac tatctgagat agaagaacac
     7501 ctgaactgta ccatttctca ggttgagccg gatataaagg taccagtgga tgaatttgat
     7561 gggaaagtta cctacggtca gaaaagggct gctggtggtg gaagctataa aggccatgtg
     7621 gatattttgg cacctactgt tcaagagttg gctgcccttg aaaaggaggc gcagacatct
     7681 ttcctgcatc ttggctacct tcctaaccag ctgttcagaa ccttcgcctc gagtctagag
     7741 ggcccgcggt tcgaaggtaa gcctatccct aaccctctcc tcggtctcga ttctacgcgt
     7801 accggtcatc atcaccatca ccattgagtt taaacgtcga caatcaacct ctggattaca
     7861 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     7921 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     7981 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     8041 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     8101 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     8161 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     8221 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     8281 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     8341 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     8401 gtcggatctc cctttgggcc gcctccccgc ctggaattct accgggtagg ggaggcgctt
     8461 ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca
     8521 agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc
     8581 tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc
     8641 ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg
     8701 cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag
     8761 cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg
     8821 ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc
     8881 tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt
     8941 tcgacctgca gcccaagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     9001 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     9061 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     9121 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     9181 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     9241 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     9301 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     9361 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     9421 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     9481 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     9541 tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga
     9601 gcgcacgacc ccatgcatct cgagggcccg gtacctttaa gaccaatgac ttacaaggca
     9661 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct agctcactcc
     9721 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     9781 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     9841 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     9901 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     9961 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
    10021 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
    10081 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
    10141 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
    10201 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
    10261 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
    10321 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
    10381 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
    10441 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
    10501 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
    10561 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
    10621 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
    10681 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
    10741 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
    10801 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
    10861 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
    10921 aaaatttaac gcgaatttta acaaaatatt aacgcttaca attt
//
