LOCUS       sequence-352384-        3483 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cry2PHR with N-terminal IBAR domain and C-terminal WH2 domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Wurz AI, Bunner WP, Szatmari EM, Hughes RM
  TITLE     CRY-BARs: Versatile light-gated molecular tools for the remodeling 
            of membrane architectures.
  JOURNAL   J Biol Chem. 2022 Aug 18;298(10):102388. doi: 
            10.1016/j.jbc.2022.102388.
  PUBMED    35987384
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2022-08-18"; volume: "298(10):102388. doi"; pages: " 
            10.1016/j.jbc.2022.102388"
COMMENT     Sequence Label: IBAR-Cry2PHR-WH2
FEATURES             Location/Qualifiers
     source          1..3483
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             763..2256
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     regulatory      2275..2284
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2281..2988
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2426..2444)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2694..2713
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atggaggctg tgattgagaa ggaatgcagc gcgctcggag gcctcttcca gaccatcatc
       61 agcgacatga aggggagcta tccagtttgg gaagatttca taaacaaagc aggaaagctg
      121 cagtcccagc ttcggacaac agtagtagca gcagctgcct tcttggacgc ctttcagaaa
      181 gtggctgaca tggccaccaa cacacgtggt gggaccaggg agattggatc tgctctcacc
      241 aggatgtgca tgaggcacag aagcattgaa gccaagctga ggcagttttc gagcgcttta
      301 attgattgtc tgataaaccc acttcaagaa cagatggaag aatggaagaa agtggccaac
      361 cagctggata aagaccacgc aaaagaatat aagaaagccc gccaagagat aaaaaagaag
      421 tcctcggata cgctgaaact gcagaagaaa gcaaaaaaag tggacactct tgggagaggt
      481 gatatccagc ctcagttgga cagtgctctc caagatgtca atgataagta tctcttattg
      541 gaagaaacag aaaagcaggc tgtccggaag gctttgattg aagaacgtgg ccgattctgt
      601 accttcatct ctatgctgcg gccagtgatt gaagaagaaa tctcaatgct aggggaaata
      661 acccaccttc agaccatctc ggaagatcta aaaagcctga ccatggaccc tcacaaactg
      721 ccctcctcaa gtgaacaggt gattctggac ctcgaggcca ccatgaagat ggacaaaaag
      781 actatagttt ggtttagaag agacctaagg attgaggata atcctgcatt agcagcagct
      841 gctcacgaag gatctgtttt tcctgtcttc atttggtgtc ctgaagaaga aggacagttt
      901 tatcctggaa gagcttcaag atggtggatg aaacaatcac ttgctcactt atctcaatcc
      961 ttgaaggctc ttggatctga cctcacttta atcaaaaccc acaacacgat ttcagcgatc
     1021 ttggattgta tccgcgttac cggtgctaca aaagtcgtct ttaaccacct ctatgatcct
     1081 gtttcgttag ttcgggacca taccgtaaag gagaagctgg tggaacgtgg gatctctgtg
     1141 caaagctaca atggagatct attgtatgaa ccgtgggaga tatactgcga aaagggcaaa
     1201 ccttttacga gtttcaattc ttactggaag aaatgcttag atatgtcgat tgaatccgtt
     1261 atgcttcctc ctccttggcg gttgatgcca ataactgcag cggctgaagc gatttgggcg
     1321 tgttcgattg aagaactagg gctggagaat gaggccgaga aaccgagcaa tgcgttgtta
     1381 actagagctt ggtctccagg atggagcaat gctgataagt tactaaatga gttcatcgag
     1441 aagcagttga tagattatgc aaagaacagc aagaaagttg ttgggaattc tacttcacta
     1501 ctttctccgt atctccattt cggggaaata agcgtcagac acgttttcca gtgtgcccgg
     1561 atgaaacaaa ttatatgggc aagagataag aacagtgaag gagaagaaag tgcagatctt
     1621 tttcttaggg gaatcggttt aagagagtat tctcggtata tatgtttcaa cttcccgttt
     1681 actcacgagc aatcgttgtt gagtcatctt cggtttttcc cttgggatgc tgatgttgat
     1741 aagttcaagg cctggagaca aggcaggacc ggttatccgt tggtggatgc cggaatgaga
     1801 gagctttggg ctaccggatg gatgcataac agaataagag tgattgtttc aagctttgct
     1861 gtgaagtttc ttctccttcc atggaaatgg ggaatgaagt atttctggga tacacttttg
     1921 gatgctgatt tggaatgtga catccttggc tggcagtata tctctgggag tatccccgat
     1981 ggccacgagc ttgatcgctt ggacaatccc gcgttacaag gcgccaaata tgacccagaa
     2041 ggtgagtaca taaggcaatg gcttcccgag cttgcgagat tgccaactga atggatccat
     2101 catccatggg acgctccttt aaccgtactc aaagcttctg gtgtggaact cggaacaaac
     2161 tatgcgaaac ccattgtaga catcgacaca gctcgtgagc tactagctaa agctatttca
     2221 agaacccgtg aagcacagat catgatcgga gcagcagccc gggatccacc ggtcgccacc
     2281 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
     2341 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
     2401 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
     2461 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
     2521 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
     2581 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
     2641 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
     2701 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
     2761 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct
     2821 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
     2881 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
     2941 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaaggg cggcagcggc
     3001 ggcagccctt ctgtccgccg gggaaccatt ggagctggtc ccatccccat caagacaccc
     3061 gtgatccctg tcaagacccc aaccgtccca gacctcccag gggtgttgcc agcccctcca
     3121 gatgggccag aagagcgcgg ggagcacagc cctgagtcgc catctgtggg tgagggcccc
     3181 caaggtgtca ccagcatgcc ctcctcaatg tggagcggcc aagcttccgt taaccctcca
     3241 cttccaggcc cgaagcccag tatccctgag gagcacagac aggcaattcc agaaagtgaa
     3301 gctgaagacc aggaacggga acccccaagt gccactgtct ccccaggcca gattccagag
     3361 agtgaccctg cagacctgag cccaagggat actccacaag gagaagacat gctgaacgcc
     3421 atccgaaggg gcgtgaaact gaagaagacc acgacaaacg atcgctcagc ccctcgcttt
     3481 tct
//
