LOCUS       sequence-35239-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  TITLE     2-series LIC N-terminal fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET LIC cloning vector for hairpin mRNA (2U-T)
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             160..182
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(329..347)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      343..390
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(756..761,762..778,779..784))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(811..829)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga
       61 ctcactatag ggagaccaca acggtttccc tctagtgccg gctccggaga gctctttaat
      121 taagcggccg ccctgcagga ctcgagttct agaaataatt ttgtttaact ttaagaagga
      181 gatatacata tgtacttcca atccaatatt ggaagtggat aacggatccg cgatcgcggc
      241 gcgccacctg gtggccggcc ggtaccacgc gtgcgcgctg atccggctgc taacaaagcc
      301 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg
      361 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca
      421 caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag
      481 caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa
      541 ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg
      601 gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc
      661 cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg
      721 actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg
      781 tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttata
//
