LOCUS       sequence-35240-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  TITLE     C-terminal fluorescent fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET Biotin His6 mCherry LIC cloning vector
            (H6...
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    69..93
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             108..130
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             143..187
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             188..205
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             206..226
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(405..423)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     673..692
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt aatacgactc
       61 actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt gtttaacttt
      121 aagaaggaga tataccatgg gaggcctgaa cgatattttt gaagcgcaga aaattgaatg
      181 gcatgaacac catcaccatc accatgaaaa cctgtacttc caatccaata ttggtagtgg
      241 gagcaacggc agcagcggat ccgtgagcaa gggcgaggag gataacatgg ccatcatcaa
      301 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
      361 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
      421 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
      481 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
      541 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
      601 ggactcctcc ctccaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
      661 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
      721 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
      781 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
      841 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tccc
//
