LOCUS       sequence-35241-e         720 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 720)
  TITLE     C-terminal fluorescent fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET Biotin His6 mOrange LIC cloning vector
            (H6...
FEATURES             Location/Qualifiers
     source          1..720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..42
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             57..79
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             92..136
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             137..154
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             155..175
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     622..641
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga aataattttg
       61 tttaacttta agaaggagat ataccatggg aggcctgaac gatatttttg aagcgcagaa
      121 aattgaatgg catgaacacc atcaccatca ccatgaaaac ctgtacttcc aatccaatat
      181 tggtagtggg agcaacggca gcagcggatc cgtgagcaag ggcgaggaga ataacatggc
      241 catcatcaag gagttcatgc gcttcaaggt gcgcatggag ggctccgtga acggccacga
      301 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggctttcaga ccgctaagct
      361 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcac
      421 ctacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tcaagctgtc
      481 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
      541 cgtgacccag gactcctccc tccaggacgg cgagttcatc tacaaggtga agctgcgcgg
      601 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
      661 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaaga tgaggctgaa
//
